Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6260 and GFF6261 overlap by 4 nucleotides GFF6261 and GFF6262 are separated by 77 nucleotides GFF6262 and GFF6263 overlap by 8 nucleotides GFF6263 and GFF6264 overlap by 4 nucleotides
GFF6260 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (EC 2.7.8.37), at 53,448 to 54,077
GFF6260
GFF6261 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG (EC 2.7.8.37), at 54,074 to 54,577
GFF6261
GFF6262 - Transcriptional regulator PhnF, at 54,655 to 55,410
GFF6262
GFF6263 - Uncharacterized protein Atu0170, clustered with phosphonate utilization, at 55,403 to 56,137
GFF6263
GFF6264 - Ribose 1,5-bisphosphate phosphokinase PhnN (EC 2.7.4.23), at 56,134 to 56,727
GFF6264
Position (kb)
54
55
56 Strain fitness (log2 ratio)
-2
-1
0
1 at 54.303 kb on + strand, within GFF6261 at 54.303 kb on + strand, within GFF6261 at 54.304 kb on - strand, within GFF6261 at 54.304 kb on - strand, within GFF6261 at 54.586 kb on + strand at 54.587 kb on - strand at 54.587 kb on - strand at 54.587 kb on - strand at 54.603 kb on - strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.632 kb on + strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.633 kb on - strand at 54.636 kb on + strand at 54.636 kb on + strand at 54.637 kb on - strand at 54.637 kb on - strand at 54.763 kb on + strand, within GFF6262 at 54.763 kb on + strand, within GFF6262 at 54.764 kb on - strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.898 kb on + strand, within GFF6262 at 54.899 kb on - strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.216 kb on + strand, within GFF6262 at 55.352 kb on - strand at 55.917 kb on - strand, within GFF6263 at 56.152 kb on + strand at 56.152 kb on + strand at 56.153 kb on - strand at 56.153 kb on - strand at 56.153 kb on - strand at 56.368 kb on + strand, within GFF6264 at 56.369 kb on - strand, within GFF6264
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 54,303 + GFF6261 0.45 -1.3 54,303 + GFF6261 0.45 -0.3 54,304 - GFF6261 0.46 +0.2 54,304 - GFF6261 0.46 -0.8 54,586 + +0.2 54,587 - +0.2 54,587 - -0.0 54,587 - -2.3 54,603 - +0.0 54,632 + +0.0 54,632 + -0.6 54,632 + +0.3 54,632 + -0.1 54,632 + -0.2 54,633 - -0.4 54,633 - -1.2 54,633 - -0.8 54,633 - -0.1 54,633 - -0.1 54,633 - -0.2 54,633 - -1.5 54,636 + -0.2 54,636 + -0.8 54,637 - -0.6 54,637 - -1.0 54,763 + GFF6262 0.14 -0.6 54,763 + GFF6262 0.14 +0.7 54,764 - GFF6262 0.14 -0.7 54,898 + GFF6262 0.32 -0.1 54,898 + GFF6262 0.32 -0.8 54,898 + GFF6262 0.32 +0.9 54,898 + GFF6262 0.32 -1.2 54,898 + GFF6262 0.32 +1.1 54,899 - GFF6262 0.32 +0.4 55,216 + GFF6262 0.74 -1.6 55,216 + GFF6262 0.74 +0.5 55,216 + GFF6262 0.74 -0.8 55,216 + GFF6262 0.74 +0.2 55,352 - -0.5 55,917 - GFF6263 0.70 +0.6 56,152 + +0.8 56,152 + -1.2 56,153 - +0.0 56,153 - -0.2 56,153 - -0.8 56,368 + GFF6264 0.39 +0.1 56,369 - GFF6264 0.40 -0.8
Or see this region's nucleotide sequence