Strain Fitness in Variovorax sp. SCN45 around GFF622

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF621 and GFF622 overlap by 4 nucleotidesGFF622 and GFF623 overlap by 4 nucleotidesGFF623 and GFF624 are separated by 76 nucleotides GFF621 - Aspartate aminotransferase (EC 2.6.1.1), at 668,367 to 669,545 GFF621 GFF622 - BUG/TctC family periplasmic protein, at 669,542 to 670,528 GFF622 GFF623 - Demethylmenaquinone methyltransferase-like protein, at 670,525 to 671,253 GFF623 GFF624 - hypothetical protein, at 671,330 to 671,680 GFF624 Position (kb) 669 670 671Strain fitness (log2 ratio) -2 -1 0 1 2 3at 668.580 kb on + strand, within GFF621at 668.856 kb on + strand, within GFF621at 668.856 kb on + strand, within GFF621at 668.856 kb on + strand, within GFF621at 668.856 kb on + strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.857 kb on - strand, within GFF621at 668.864 kb on + strand, within GFF621at 668.864 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.934 kb on + strand, within GFF621at 668.935 kb on - strand, within GFF621at 668.949 kb on + strand, within GFF621at 668.949 kb on + strand, within GFF621at 668.949 kb on + strand, within GFF621at 668.950 kb on - strand, within GFF621at 668.950 kb on - strand, within GFF621at 669.036 kb on + strand, within GFF621at 669.037 kb on - strand, within GFF621at 669.288 kb on + strand, within GFF621at 669.288 kb on + strand, within GFF621at 669.289 kb on - strand, within GFF621at 669.289 kb on - strand, within GFF621at 669.417 kb on + strand, within GFF621at 669.932 kb on + strand, within GFF622at 669.933 kb on - strand, within GFF622at 669.933 kb on - strand, within GFF622at 669.933 kb on - strand, within GFF622at 669.933 kb on - strand, within GFF622at 669.989 kb on + strand, within GFF622at 669.990 kb on - strand, within GFF622at 670.106 kb on + strand, within GFF622at 670.107 kb on - strand, within GFF622at 670.107 kb on - strand, within GFF622at 670.107 kb on - strand, within GFF622at 670.107 kb on - strand, within GFF622at 670.107 kb on - strand, within GFF622at 671.090 kb on + strand, within GFF623at 671.091 kb on - strand, within GFF623at 671.331 kb on + strandat 671.331 kb on + strandat 671.331 kb on + strandat 671.331 kb on + strandat 671.331 kb on + strandat 671.331 kb on + strandat 671.332 kb on - strandat 671.332 kb on - strandat 671.332 kb on - strandat 671.374 kb on - strand, within GFF624at 671.520 kb on + strand, within GFF624

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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668,580 + GFF621 0.18 +0.4
668,856 + GFF621 0.41 -0.6
668,856 + GFF621 0.41 -0.5
668,856 + GFF621 0.41 +0.3
668,856 + GFF621 0.41 -0.0
668,857 - GFF621 0.42 -0.4
668,857 - GFF621 0.42 +0.4
668,857 - GFF621 0.42 +0.2
668,857 - GFF621 0.42 -0.5
668,857 - GFF621 0.42 +1.4
668,857 - GFF621 0.42 -0.4
668,857 - GFF621 0.42 -0.3
668,864 + GFF621 0.42 -0.3
668,864 + GFF621 0.42 -0.5
668,934 + GFF621 0.48 -0.2
668,934 + GFF621 0.48 -2.1
668,934 + GFF621 0.48 -0.1
668,934 + GFF621 0.48 -1.0
668,934 + GFF621 0.48 -0.6
668,934 + GFF621 0.48 +0.2
668,935 - GFF621 0.48 -0.8
668,949 + GFF621 0.49 -2.2
668,949 + GFF621 0.49 -0.5
668,949 + GFF621 0.49 +0.6
668,950 - GFF621 0.49 -1.2
668,950 - GFF621 0.49 -0.9
669,036 + GFF621 0.57 -0.4
669,037 - GFF621 0.57 +0.0
669,288 + GFF621 0.78 +0.6
669,288 + GFF621 0.78 -0.6
669,289 - GFF621 0.78 +0.4
669,289 - GFF621 0.78 +0.3
669,417 + GFF621 0.89 +0.1
669,932 + GFF622 0.40 -0.9
669,933 - GFF622 0.40 +0.1
669,933 - GFF622 0.40 -0.7
669,933 - GFF622 0.40 -0.5
669,933 - GFF622 0.40 -0.4
669,989 + GFF622 0.45 -0.6
669,990 - GFF622 0.45 -1.3
670,106 + GFF622 0.57 +0.4
670,107 - GFF622 0.57 -0.7
670,107 - GFF622 0.57 +0.3
670,107 - GFF622 0.57 -0.1
670,107 - GFF622 0.57 +0.3
670,107 - GFF622 0.57 +0.2
671,090 + GFF623 0.78 +0.5
671,091 - GFF623 0.78 +0.4
671,331 + -1.2
671,331 + +0.2
671,331 + -0.5
671,331 + -0.9
671,331 + +0.2
671,331 + -0.8
671,332 - +2.9
671,332 - -0.2
671,332 - +0.6
671,374 - GFF624 0.13 -0.7
671,520 + GFF624 0.54 -0.5

Or see this region's nucleotide sequence