Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6219 and GFF6220 are separated by 153 nucleotides
GFF6219 - hypothetical protein, at 127,859 to 132,379
GFF6219
GFF6220 - hypothetical protein, at 132,533 to 135,472
GFF6220
Position (kb)
127
128
129
130
131
132
133 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.920 kb on + strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.921 kb on - strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.955 kb on + strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 126.956 kb on - strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.102 kb on + strand at 127.103 kb on - strand at 127.103 kb on - strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.166 kb on + strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.167 kb on - strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.809 kb on - strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.904 kb on + strand at 127.905 kb on - strand at 127.905 kb on - strand at 127.998 kb on + strand at 127.998 kb on + strand at 127.998 kb on + strand at 127.999 kb on - strand at 127.999 kb on - strand at 128.150 kb on + strand at 128.150 kb on + strand at 128.150 kb on + strand at 128.150 kb on + strand at 128.151 kb on - strand at 128.151 kb on - strand at 128.151 kb on - strand at 128.151 kb on - strand at 128.396 kb on + strand, within GFF6219 at 128.396 kb on + strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.397 kb on - strand, within GFF6219 at 128.618 kb on - strand, within GFF6219 at 128.642 kb on + strand, within GFF6219 at 128.643 kb on - strand, within GFF6219 at 128.643 kb on - strand, within GFF6219 at 128.643 kb on - strand, within GFF6219 at 128.665 kb on + strand, within GFF6219 at 128.888 kb on + strand, within GFF6219 at 128.888 kb on + strand, within GFF6219 at 128.889 kb on - strand, within GFF6219 at 128.889 kb on - strand, within GFF6219 at 128.889 kb on - strand, within GFF6219 at 128.988 kb on + strand, within GFF6219 at 128.988 kb on + strand, within GFF6219 at 128.989 kb on - strand, within GFF6219 at 129.134 kb on + strand, within GFF6219 at 129.134 kb on + strand, within GFF6219 at 129.134 kb on + strand, within GFF6219 at 129.135 kb on - strand, within GFF6219 at 129.135 kb on - strand, within GFF6219 at 129.380 kb on + strand, within GFF6219 at 129.380 kb on + strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.381 kb on - strand, within GFF6219 at 129.626 kb on + strand, within GFF6219 at 129.627 kb on - strand, within GFF6219 at 129.889 kb on + strand, within GFF6219 at 129.889 kb on + strand, within GFF6219 at 129.889 kb on + strand, within GFF6219 at 129.890 kb on - strand, within GFF6219 at 129.890 kb on - strand, within GFF6219 at 129.890 kb on - strand, within GFF6219 at 130.279 kb on + strand, within GFF6219 at 130.365 kb on - strand, within GFF6219 at 130.610 kb on + strand, within GFF6219 at 130.610 kb on + strand, within GFF6219 at 130.610 kb on + strand, within GFF6219 at 130.610 kb on + strand, within GFF6219 at 130.611 kb on - strand, within GFF6219 at 130.611 kb on - strand, within GFF6219 at 130.611 kb on - strand, within GFF6219 at 130.611 kb on - strand, within GFF6219 at 130.856 kb on + strand, within GFF6219 at 130.857 kb on - strand, within GFF6219 at 131.147 kb on + strand, within GFF6219 at 131.147 kb on + strand, within GFF6219 at 131.147 kb on + strand, within GFF6219 at 131.147 kb on + strand, within GFF6219 at 131.147 kb on + strand, within GFF6219 at 131.148 kb on - strand, within GFF6219 at 131.348 kb on + strand, within GFF6219 at 131.348 kb on + strand, within GFF6219 at 131.348 kb on + strand, within GFF6219 at 131.349 kb on - strand, within GFF6219 at 131.349 kb on - strand, within GFF6219 at 131.349 kb on - strand, within GFF6219 at 131.572 kb on + strand, within GFF6219 at 131.594 kb on + strand, within GFF6219 at 131.594 kb on + strand, within GFF6219 at 131.594 kb on + strand, within GFF6219 at 131.595 kb on - strand, within GFF6219 at 131.595 kb on - strand, within GFF6219 at 131.595 kb on - strand, within GFF6219 at 131.595 kb on - strand, within GFF6219 at 131.595 kb on - strand, within GFF6219 at 131.840 kb on + strand, within GFF6219 at 131.840 kb on + strand, within GFF6219 at 131.840 kb on + strand, within GFF6219 at 131.840 kb on + strand, within GFF6219 at 131.840 kb on + strand, within GFF6219 at 131.841 kb on - strand, within GFF6219 at 131.841 kb on - strand, within GFF6219 at 131.841 kb on - strand, within GFF6219 at 132.086 kb on + strand at 132.086 kb on + strand at 132.333 kb on - strand at 132.333 kb on - strand at 132.333 kb on - strand at 132.432 kb on + strand at 132.824 kb on + strand at 132.824 kb on + strand at 132.824 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 126,920 + +0.4 126,920 + -1.2 126,920 + -0.6 126,920 + +0.4 126,920 + +0.3 126,920 + -1.2 126,920 + -0.3 126,920 + +0.0 126,920 + +2.0 126,921 - -1.4 126,921 - -0.5 126,921 - -1.1 126,921 - +0.5 126,921 - -0.2 126,921 - -0.1 126,921 - -0.3 126,921 - -1.2 126,921 - -0.4 126,921 - +1.1 126,921 - +0.6 126,921 - -0.5 126,955 + -0.1 126,955 + +0.9 126,955 + -0.4 126,955 + +0.9 126,955 + -0.2 126,955 + -0.4 126,955 + -1.5 126,956 - -1.0 126,956 - +1.9 126,956 - +0.3 126,956 - +0.1 126,956 - +0.9 127,102 + -0.1 127,102 + +0.7 127,102 + -1.0 127,102 + +0.3 127,103 - -0.3 127,103 - -0.1 127,166 + -0.4 127,166 + -0.7 127,166 + -0.3 127,166 + +1.0 127,166 + -1.6 127,167 - -0.9 127,167 - -0.9 127,167 - -0.1 127,167 - -0.2 127,412 + -0.5 127,412 + +0.2 127,412 + +0.3 127,412 + -0.3 127,412 + +0.5 127,412 + -1.1 127,412 + +0.1 127,412 + -0.5 127,413 - -1.3 127,413 - +0.1 127,413 - -0.9 127,809 - -0.2 127,904 + -1.0 127,904 + +0.1 127,904 + -0.2 127,904 + -1.6 127,904 + -0.1 127,904 + -0.9 127,904 + -0.3 127,904 + +0.4 127,905 - -0.2 127,905 - +0.9 127,998 + -0.1 127,998 + -0.1 127,998 + -0.3 127,999 - -0.7 127,999 - -0.5 128,150 + +0.1 128,150 + -0.8 128,150 + -0.2 128,150 + +1.7 128,151 - -0.7 128,151 - -0.3 128,151 - +0.7 128,151 - -0.3 128,396 + GFF6219 0.12 -0.4 128,396 + GFF6219 0.12 +0.2 128,397 - GFF6219 0.12 +0.5 128,397 - GFF6219 0.12 -0.3 128,397 - GFF6219 0.12 -0.9 128,397 - GFF6219 0.12 -0.7 128,397 - GFF6219 0.12 -2.3 128,397 - GFF6219 0.12 -0.7 128,397 - GFF6219 0.12 +0.5 128,397 - GFF6219 0.12 -0.1 128,397 - GFF6219 0.12 +1.4 128,618 - GFF6219 0.17 -1.3 128,642 + GFF6219 0.17 +0.4 128,643 - GFF6219 0.17 -1.4 128,643 - GFF6219 0.17 +3.5 128,643 - GFF6219 0.17 -0.3 128,665 + GFF6219 0.18 -0.3 128,888 + GFF6219 0.23 -1.1 128,888 + GFF6219 0.23 -0.2 128,889 - GFF6219 0.23 -0.9 128,889 - GFF6219 0.23 +0.9 128,889 - GFF6219 0.23 +0.3 128,988 + GFF6219 0.25 -0.3 128,988 + GFF6219 0.25 -1.1 128,989 - GFF6219 0.25 -0.5 129,134 + GFF6219 0.28 -0.7 129,134 + GFF6219 0.28 -0.4 129,134 + GFF6219 0.28 +1.3 129,135 - GFF6219 0.28 +0.5 129,135 - GFF6219 0.28 -1.1 129,380 + GFF6219 0.34 +0.2 129,380 + GFF6219 0.34 -0.6 129,381 - GFF6219 0.34 -0.2 129,381 - GFF6219 0.34 -0.2 129,381 - GFF6219 0.34 -0.6 129,381 - GFF6219 0.34 -1.1 129,381 - GFF6219 0.34 +0.5 129,381 - GFF6219 0.34 +0.3 129,626 + GFF6219 0.39 -0.4 129,627 - GFF6219 0.39 -0.1 129,889 + GFF6219 0.45 -1.5 129,889 + GFF6219 0.45 -1.2 129,889 + GFF6219 0.45 +0.1 129,890 - GFF6219 0.45 -2.5 129,890 - GFF6219 0.45 -0.1 129,890 - GFF6219 0.45 -1.2 130,279 + GFF6219 0.54 -1.1 130,365 - GFF6219 0.55 -0.3 130,610 + GFF6219 0.61 +0.5 130,610 + GFF6219 0.61 -0.4 130,610 + GFF6219 0.61 -0.2 130,610 + GFF6219 0.61 +0.6 130,611 - GFF6219 0.61 +0.1 130,611 - GFF6219 0.61 +0.8 130,611 - GFF6219 0.61 +0.6 130,611 - GFF6219 0.61 +1.1 130,856 + GFF6219 0.66 -0.0 130,857 - GFF6219 0.66 +0.5 131,147 + GFF6219 0.73 -0.5 131,147 + GFF6219 0.73 -0.7 131,147 + GFF6219 0.73 +0.2 131,147 + GFF6219 0.73 -0.6 131,147 + GFF6219 0.73 -0.6 131,148 - GFF6219 0.73 -0.1 131,348 + GFF6219 0.77 +0.3 131,348 + GFF6219 0.77 -1.1 131,348 + GFF6219 0.77 -0.0 131,349 - GFF6219 0.77 +2.3 131,349 - GFF6219 0.77 +0.6 131,349 - GFF6219 0.77 -0.3 131,572 + GFF6219 0.82 +0.0 131,594 + GFF6219 0.83 -2.3 131,594 + GFF6219 0.83 -0.1 131,594 + GFF6219 0.83 +0.1 131,595 - GFF6219 0.83 +0.5 131,595 - GFF6219 0.83 +1.2 131,595 - GFF6219 0.83 -0.8 131,595 - GFF6219 0.83 -1.6 131,595 - GFF6219 0.83 -1.3 131,840 + GFF6219 0.88 -0.5 131,840 + GFF6219 0.88 -0.4 131,840 + GFF6219 0.88 -1.0 131,840 + GFF6219 0.88 +0.1 131,840 + GFF6219 0.88 -1.0 131,841 - GFF6219 0.88 -0.2 131,841 - GFF6219 0.88 -0.1 131,841 - GFF6219 0.88 -1.1 132,086 + -0.8 132,086 + -1.6 132,333 - -0.0 132,333 - -0.4 132,333 - +0.7 132,432 + +1.0 132,824 + -0.1 132,824 + -0.4 132,824 + -1.4
Or see this region's nucleotide sequence