Strain Fitness in Variovorax sp. SCN45 around GFF6061

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6060 and GFF6061 overlap by 4 nucleotidesGFF6061 and GFF6062 are separated by 64 nucleotides GFF6060 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 121,544 to 122,608 GFF6060 GFF6061 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 122,605 to 123,468 GFF6061 GFF6062 - Sodium-dependent phosphate transporter, at 123,533 to 125,194 GFF6062 Position (kb) 122 123 124Strain fitness (log2 ratio) -1 0 1 2at 121.811 kb on + strand, within GFF6060at 121.811 kb on + strand, within GFF6060at 121.812 kb on - strand, within GFF6060at 121.812 kb on - strand, within GFF6060at 122.689 kb on + strandat 122.689 kb on + strandat 122.690 kb on - strandat 122.690 kb on - strandat 122.713 kb on + strand, within GFF6061at 122.812 kb on + strand, within GFF6061at 122.813 kb on - strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 123.417 kb on + strandat 123.518 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.583 kb on + strandat 123.598 kb on + strandat 123.615 kb on + strandat 123.616 kb on - strandat 123.822 kb on - strand, within GFF6062at 124.319 kb on + strand, within GFF6062at 124.319 kb on + strand, within GFF6062at 124.319 kb on + strand, within GFF6062at 124.319 kb on + strand, within GFF6062at 124.320 kb on - strand, within GFF6062at 124.320 kb on - strand, within GFF6062

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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121,811 + GFF6060 0.25 +1.3
121,811 + GFF6060 0.25 -0.7
121,812 - GFF6060 0.25 -1.2
121,812 - GFF6060 0.25 -0.2
122,689 + +0.7
122,689 + +0.0
122,690 - -0.3
122,690 - -0.1
122,713 + GFF6061 0.12 -1.5
122,812 + GFF6061 0.24 +0.0
122,813 - GFF6061 0.24 -0.3
122,857 + GFF6061 0.29 -1.2
122,857 + GFF6061 0.29 -1.0
122,857 + GFF6061 0.29 -0.6
122,920 + GFF6061 0.36 +0.7
122,920 + GFF6061 0.36 +0.0
122,920 + GFF6061 0.36 -0.6
122,921 - GFF6061 0.37 -0.4
122,921 - GFF6061 0.37 -1.4
122,921 - GFF6061 0.37 -0.7
123,417 + -0.7
123,518 + -0.5
123,543 + -1.3
123,543 + -0.8
123,543 + +0.1
123,543 + -1.7
123,544 - -0.7
123,544 - -0.0
123,544 - -0.7
123,544 - -0.3
123,544 - -0.1
123,544 - -0.3
123,544 - +0.7
123,583 + +1.9
123,598 + -1.2
123,615 + +0.4
123,616 - +0.0
123,822 - GFF6062 0.17 +1.6
124,319 + GFF6062 0.47 -1.5
124,319 + GFF6062 0.47 -0.3
124,319 + GFF6062 0.47 -1.3
124,319 + GFF6062 0.47 -0.7
124,320 - GFF6062 0.47 -0.0
124,320 - GFF6062 0.47 -0.8

Or see this region's nucleotide sequence