Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6043 and GFF6044 are separated by 41 nucleotides GFF6044 and GFF6045 are separated by 50 nucleotides GFF6045 and GFF6046 are separated by 40 nucleotides GFF6046 and GFF6047 are separated by 36 nucleotides
GFF6043 - tRNA/rRNA cytosine-C5-methylase, at 103,212 to 104,483
GFF6043
GFF6044 - FIG00933533: hypothetical protein, at 104,525 to 106,378
GFF6044
GFF6045 - FIG00932121: hypothetical protein, at 106,429 to 106,665
GFF6045
GFF6046 - hypothetical protein, at 106,706 to 107,032
GFF6046
GFF6047 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 107,069 to 107,665
GFF6047
Position (kb) 104
105
106
107 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 103.569 kb on - strand, within GFF6043 at 103.741 kb on + strand, within GFF6043 at 103.741 kb on + strand, within GFF6043 at 103.742 kb on - strand, within GFF6043 at 103.742 kb on - strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.757 kb on + strand, within GFF6043 at 103.758 kb on - strand, within GFF6043 at 103.758 kb on - strand, within GFF6043 at 103.758 kb on - strand, within GFF6043 at 103.758 kb on - strand, within GFF6043 at 103.758 kb on - strand, within GFF6043 at 104.242 kb on + strand, within GFF6043 at 104.242 kb on + strand, within GFF6043 at 104.242 kb on + strand, within GFF6043 at 104.242 kb on + strand, within GFF6043 at 104.242 kb on + strand, within GFF6043 at 104.243 kb on - strand, within GFF6043 at 104.243 kb on - strand, within GFF6043 at 104.243 kb on - strand, within GFF6043 at 104.243 kb on - strand, within GFF6043 at 104.332 kb on + strand, within GFF6043 at 104.332 kb on + strand, within GFF6043 at 104.332 kb on + strand, within GFF6043 at 104.332 kb on + strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.333 kb on - strand, within GFF6043 at 104.526 kb on + strand at 104.526 kb on + strand at 104.526 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.703 kb on + strand at 104.704 kb on - strand at 104.704 kb on - strand at 104.704 kb on - strand at 104.704 kb on - strand at 104.704 kb on - strand at 104.805 kb on + strand, within GFF6044 at 104.805 kb on + strand, within GFF6044 at 104.806 kb on - strand, within GFF6044 at 104.806 kb on - strand, within GFF6044 at 104.806 kb on - strand, within GFF6044 at 104.806 kb on - strand, within GFF6044 at 104.806 kb on - strand, within GFF6044 at 105.123 kb on + strand, within GFF6044 at 105.124 kb on - strand, within GFF6044 at 105.229 kb on + strand, within GFF6044 at 105.229 kb on + strand, within GFF6044 at 105.229 kb on + strand, within GFF6044 at 105.229 kb on + strand, within GFF6044 at 105.229 kb on + strand, within GFF6044 at 105.230 kb on - strand, within GFF6044 at 105.230 kb on - strand, within GFF6044 at 105.230 kb on - strand, within GFF6044 at 105.309 kb on + strand, within GFF6044 at 105.309 kb on + strand, within GFF6044 at 105.588 kb on + strand, within GFF6044 at 105.588 kb on + strand, within GFF6044 at 105.588 kb on + strand, within GFF6044 at 105.589 kb on - strand, within GFF6044 at 105.589 kb on - strand, within GFF6044 at 105.589 kb on - strand, within GFF6044 at 105.675 kb on + strand, within GFF6044 at 105.675 kb on + strand, within GFF6044 at 105.675 kb on + strand, within GFF6044 at 105.675 kb on + strand, within GFF6044 at 105.676 kb on - strand, within GFF6044 at 105.676 kb on - strand, within GFF6044 at 105.676 kb on - strand, within GFF6044 at 105.676 kb on - strand, within GFF6044 at 105.726 kb on + strand, within GFF6044 at 105.726 kb on + strand, within GFF6044 at 105.726 kb on + strand, within GFF6044 at 105.726 kb on + strand, within GFF6044 at 105.727 kb on - strand, within GFF6044 at 105.727 kb on - strand, within GFF6044 at 105.727 kb on - strand, within GFF6044 at 105.727 kb on - strand, within GFF6044 at 105.819 kb on + strand, within GFF6044 at 105.819 kb on + strand, within GFF6044 at 105.819 kb on + strand, within GFF6044 at 105.819 kb on + strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.820 kb on - strand, within GFF6044 at 105.864 kb on + strand, within GFF6044 at 105.864 kb on + strand, within GFF6044 at 105.864 kb on + strand, within GFF6044 at 105.865 kb on - strand, within GFF6044 at 106.004 kb on + strand, within GFF6044 at 106.004 kb on + strand, within GFF6044 at 106.004 kb on + strand, within GFF6044 at 106.005 kb on - strand, within GFF6044 at 106.005 kb on - strand, within GFF6044 at 106.005 kb on - strand, within GFF6044 at 106.005 kb on - strand, within GFF6044 at 106.008 kb on + strand, within GFF6044 at 106.009 kb on - strand, within GFF6044 at 106.272 kb on + strand at 106.272 kb on + strand at 106.272 kb on + strand at 106.273 kb on - strand at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.489 kb on + strand, within GFF6045 at 106.490 kb on - strand, within GFF6045 at 106.490 kb on - strand, within GFF6045 at 106.504 kb on + strand, within GFF6045 at 106.504 kb on + strand, within GFF6045 at 106.504 kb on + strand, within GFF6045 at 106.505 kb on - strand, within GFF6045 at 106.505 kb on - strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.570 kb on + strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.571 kb on - strand, within GFF6045 at 106.663 kb on + strand at 106.664 kb on - strand at 106.664 kb on - strand at 106.664 kb on - strand at 106.664 kb on - strand at 106.664 kb on - strand at 106.908 kb on + strand, within GFF6046 at 106.908 kb on + strand, within GFF6046 at 106.908 kb on + strand, within GFF6046 at 106.909 kb on - strand, within GFF6046 at 106.909 kb on - strand, within GFF6046 at 106.909 kb on - strand, within GFF6046 at 106.909 kb on - strand, within GFF6046 at 107.057 kb on + strand at 107.058 kb on - strand at 107.058 kb on - strand at 107.058 kb on - strand at 107.058 kb on - strand at 107.059 kb on + strand at 107.059 kb on + strand at 107.059 kb on + strand at 107.060 kb on - strand at 107.060 kb on - strand at 107.060 kb on - strand at 107.070 kb on + strand at 107.071 kb on - strand at 107.071 kb on - strand at 107.071 kb on - strand at 107.089 kb on + strand at 107.089 kb on + strand at 107.089 kb on + strand at 107.090 kb on - strand at 107.090 kb on - strand at 107.090 kb on - strand at 107.090 kb on - strand at 107.121 kb on + strand at 107.121 kb on + strand at 107.122 kb on - strand at 107.122 kb on - strand at 107.122 kb on - strand at 107.234 kb on + strand, within GFF6047 at 107.234 kb on + strand, within GFF6047 at 107.234 kb on + strand, within GFF6047 at 107.235 kb on - strand, within GFF6047 at 107.352 kb on + strand, within GFF6047 at 107.352 kb on + strand, within GFF6047
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 103,569 - GFF6043 0.28 -0.3 103,741 + GFF6043 0.42 -0.2 103,741 + GFF6043 0.42 -0.3 103,742 - GFF6043 0.42 -0.8 103,742 - GFF6043 0.42 -0.5 103,757 + GFF6043 0.43 -0.4 103,757 + GFF6043 0.43 +0.1 103,757 + GFF6043 0.43 +0.0 103,757 + GFF6043 0.43 -0.6 103,757 + GFF6043 0.43 -0.3 103,757 + GFF6043 0.43 -0.4 103,758 - GFF6043 0.43 -0.4 103,758 - GFF6043 0.43 -0.1 103,758 - GFF6043 0.43 -0.2 103,758 - GFF6043 0.43 -0.6 103,758 - GFF6043 0.43 -1.6 104,242 + GFF6043 0.81 -0.9 104,242 + GFF6043 0.81 -0.5 104,242 + GFF6043 0.81 -0.9 104,242 + GFF6043 0.81 +0.0 104,242 + GFF6043 0.81 +0.1 104,243 - GFF6043 0.81 -0.4 104,243 - GFF6043 0.81 -0.8 104,243 - GFF6043 0.81 +0.6 104,243 - GFF6043 0.81 -0.5 104,332 + GFF6043 0.88 +0.4 104,332 + GFF6043 0.88 +0.6 104,332 + GFF6043 0.88 -1.8 104,332 + GFF6043 0.88 +0.6 104,333 - GFF6043 0.88 +1.0 104,333 - GFF6043 0.88 +1.3 104,333 - GFF6043 0.88 -0.2 104,333 - GFF6043 0.88 -1.0 104,333 - GFF6043 0.88 +0.4 104,333 - GFF6043 0.88 +0.6 104,526 + -0.3 104,526 + +2.3 104,526 + -2.0 104,703 + -0.4 104,703 + +1.6 104,703 + -1.0 104,703 + +2.9 104,703 + +0.8 104,703 + -0.4 104,703 + +0.9 104,703 + +1.5 104,703 + -1.2 104,703 + -1.2 104,703 + -0.4 104,704 - -0.0 104,704 - +1.6 104,704 - +1.9 104,704 - +0.8 104,704 - +0.6 104,805 + GFF6044 0.15 +1.1 104,805 + GFF6044 0.15 -0.6 104,806 - GFF6044 0.15 +0.1 104,806 - GFF6044 0.15 -0.9 104,806 - GFF6044 0.15 +0.1 104,806 - GFF6044 0.15 +0.1 104,806 - GFF6044 0.15 -0.7 105,123 + GFF6044 0.32 -0.1 105,124 - GFF6044 0.32 -0.2 105,229 + GFF6044 0.38 -0.5 105,229 + GFF6044 0.38 -0.8 105,229 + GFF6044 0.38 -1.6 105,229 + GFF6044 0.38 +1.2 105,229 + GFF6044 0.38 -1.2 105,230 - GFF6044 0.38 -0.7 105,230 - GFF6044 0.38 -0.2 105,230 - GFF6044 0.38 -0.3 105,309 + GFF6044 0.42 -0.2 105,309 + GFF6044 0.42 +0.2 105,588 + GFF6044 0.57 +0.1 105,588 + GFF6044 0.57 -0.9 105,588 + GFF6044 0.57 -1.7 105,589 - GFF6044 0.57 -0.1 105,589 - GFF6044 0.57 -0.6 105,589 - GFF6044 0.57 -0.4 105,675 + GFF6044 0.62 -1.0 105,675 + GFF6044 0.62 -0.2 105,675 + GFF6044 0.62 -0.0 105,675 + GFF6044 0.62 -1.4 105,676 - GFF6044 0.62 -0.5 105,676 - GFF6044 0.62 +0.4 105,676 - GFF6044 0.62 -2.2 105,676 - GFF6044 0.62 -0.3 105,726 + GFF6044 0.65 -0.4 105,726 + GFF6044 0.65 +0.5 105,726 + GFF6044 0.65 -0.3 105,726 + GFF6044 0.65 -0.4 105,727 - GFF6044 0.65 -0.7 105,727 - GFF6044 0.65 +1.1 105,727 - GFF6044 0.65 +0.5 105,727 - GFF6044 0.65 -0.6 105,819 + GFF6044 0.70 +0.6 105,819 + GFF6044 0.70 -0.5 105,819 + GFF6044 0.70 -0.2 105,819 + GFF6044 0.70 +0.2 105,820 - GFF6044 0.70 +0.7 105,820 - GFF6044 0.70 -0.1 105,820 - GFF6044 0.70 +0.0 105,820 - GFF6044 0.70 +2.6 105,820 - GFF6044 0.70 +0.5 105,820 - GFF6044 0.70 -0.5 105,820 - GFF6044 0.70 +0.3 105,864 + GFF6044 0.72 +0.2 105,864 + GFF6044 0.72 -1.2 105,864 + GFF6044 0.72 -0.3 105,865 - GFF6044 0.72 -0.3 106,004 + GFF6044 0.80 +1.0 106,004 + GFF6044 0.80 +1.6 106,004 + GFF6044 0.80 +0.6 106,005 - GFF6044 0.80 -0.7 106,005 - GFF6044 0.80 +0.3 106,005 - GFF6044 0.80 -1.0 106,005 - GFF6044 0.80 -1.5 106,008 + GFF6044 0.80 -1.1 106,009 - GFF6044 0.80 +0.2 106,272 + -0.3 106,272 + -0.1 106,272 + +2.2 106,273 - +0.7 106,489 + GFF6045 0.25 -0.5 106,489 + GFF6045 0.25 -0.8 106,489 + GFF6045 0.25 -1.1 106,489 + GFF6045 0.25 -1.0 106,489 + GFF6045 0.25 -0.2 106,489 + GFF6045 0.25 +0.6 106,489 + GFF6045 0.25 +0.1 106,490 - GFF6045 0.26 -0.4 106,490 - GFF6045 0.26 -0.3 106,504 + GFF6045 0.32 -0.8 106,504 + GFF6045 0.32 +0.9 106,504 + GFF6045 0.32 -0.5 106,505 - GFF6045 0.32 -0.5 106,505 - GFF6045 0.32 +0.5 106,570 + GFF6045 0.59 -0.6 106,570 + GFF6045 0.59 +0.0 106,570 + GFF6045 0.59 -0.1 106,570 + GFF6045 0.59 -0.3 106,570 + GFF6045 0.59 -1.2 106,570 + GFF6045 0.59 +0.0 106,570 + GFF6045 0.59 -0.4 106,570 + GFF6045 0.59 -0.5 106,570 + GFF6045 0.59 -0.2 106,570 + GFF6045 0.59 -0.2 106,570 + GFF6045 0.59 -0.5 106,571 - GFF6045 0.60 +0.7 106,571 - GFF6045 0.60 -1.3 106,571 - GFF6045 0.60 -0.3 106,571 - GFF6045 0.60 -0.4 106,571 - GFF6045 0.60 -2.0 106,571 - GFF6045 0.60 +0.9 106,571 - GFF6045 0.60 +0.3 106,571 - GFF6045 0.60 -0.0 106,663 + -1.4 106,664 - -0.4 106,664 - -0.1 106,664 - +0.6 106,664 - -0.1 106,664 - -0.5 106,908 + GFF6046 0.62 -1.0 106,908 + GFF6046 0.62 +0.0 106,908 + GFF6046 0.62 -1.1 106,909 - GFF6046 0.62 -0.3 106,909 - GFF6046 0.62 -1.0 106,909 - GFF6046 0.62 -0.4 106,909 - GFF6046 0.62 +0.0 107,057 + +0.3 107,058 - +0.6 107,058 - +0.2 107,058 - -0.9 107,058 - -0.9 107,059 + -0.5 107,059 + -0.1 107,059 + -0.3 107,060 - -0.9 107,060 - -1.5 107,060 - -1.3 107,070 + -0.5 107,071 - +0.3 107,071 - -0.8 107,071 - -1.5 107,089 + -0.9 107,089 + -0.5 107,089 + -0.4 107,090 - +0.1 107,090 - +0.9 107,090 - -0.8 107,090 - +0.5 107,121 + -0.4 107,121 + +0.2 107,122 - +3.6 107,122 - -0.5 107,122 - -0.4 107,234 + GFF6047 0.28 +0.1 107,234 + GFF6047 0.28 -0.7 107,234 + GFF6047 0.28 +0.1 107,235 - GFF6047 0.28 +0.9 107,352 + GFF6047 0.47 +1.6 107,352 + GFF6047 0.47 +0.0
Or see this region's nucleotide sequence