Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6028 and GFF6029 are separated by 28 nucleotides GFF6029 and GFF6030 are separated by 0 nucleotides GFF6030 and GFF6031 are separated by 1 nucleotides
GFF6028 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 88,590 to 89,654
GFF6028
GFF6029 - BUG/TctC family periplasmic protein, at 89,683 to 90,663
GFF6029
GFF6030 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 90,664 to 91,440
GFF6030
GFF6031 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 91,442 to 92,221
GFF6031
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 88.716 kb on + strand, within GFF6028 at 89.022 kb on + strand, within GFF6028 at 89.022 kb on + strand, within GFF6028 at 89.023 kb on - strand, within GFF6028 at 89.023 kb on - strand, within GFF6028 at 89.023 kb on - strand, within GFF6028 at 89.023 kb on - strand, within GFF6028 at 89.061 kb on + strand, within GFF6028 at 89.061 kb on + strand, within GFF6028 at 89.062 kb on - strand, within GFF6028 at 89.084 kb on + strand, within GFF6028 at 89.084 kb on + strand, within GFF6028 at 89.084 kb on + strand, within GFF6028 at 89.338 kb on - strand, within GFF6028 at 89.338 kb on - strand, within GFF6028 at 89.338 kb on - strand, within GFF6028 at 89.338 kb on - strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.367 kb on + strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.368 kb on - strand, within GFF6028 at 89.383 kb on + strand, within GFF6028 at 89.383 kb on + strand, within GFF6028 at 89.384 kb on - strand, within GFF6028 at 89.418 kb on + strand, within GFF6028 at 89.418 kb on + strand, within GFF6028 at 89.419 kb on - strand, within GFF6028 at 89.529 kb on + strand, within GFF6028 at 89.529 kb on + strand, within GFF6028 at 89.529 kb on + strand, within GFF6028 at 89.529 kb on + strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.530 kb on - strand, within GFF6028 at 89.574 kb on + strand at 89.575 kb on - strand at 89.575 kb on - strand at 89.575 kb on - strand at 89.575 kb on - strand at 89.665 kb on - strand at 89.665 kb on - strand at 89.665 kb on - strand at 89.668 kb on + strand at 89.668 kb on + strand at 89.669 kb on - strand at 89.669 kb on - strand at 89.669 kb on - strand at 89.669 kb on - strand at 89.794 kb on + strand, within GFF6029 at 89.992 kb on + strand, within GFF6029 at 89.992 kb on + strand, within GFF6029 at 89.992 kb on + strand, within GFF6029 at 89.992 kb on + strand, within GFF6029 at 89.993 kb on - strand, within GFF6029 at 89.993 kb on - strand, within GFF6029 at 90.128 kb on - strand, within GFF6029 at 90.157 kb on + strand, within GFF6029 at 90.158 kb on - strand, within GFF6029 at 90.158 kb on - strand, within GFF6029 at 90.158 kb on - strand, within GFF6029 at 90.244 kb on + strand, within GFF6029 at 90.244 kb on + strand, within GFF6029 at 90.245 kb on - strand, within GFF6029 at 90.245 kb on - strand, within GFF6029 at 90.269 kb on + strand, within GFF6029 at 90.269 kb on + strand, within GFF6029 at 90.269 kb on + strand, within GFF6029 at 90.269 kb on + strand, within GFF6029 at 90.270 kb on - strand, within GFF6029 at 90.270 kb on - strand, within GFF6029 at 90.622 kb on + strand at 90.622 kb on + strand at 90.622 kb on + strand at 90.622 kb on + strand at 90.677 kb on + strand at 90.755 kb on + strand, within GFF6030 at 90.755 kb on + strand, within GFF6030 at 90.756 kb on - strand, within GFF6030 at 90.756 kb on - strand, within GFF6030 at 90.756 kb on - strand, within GFF6030 at 90.756 kb on - strand, within GFF6030 at 91.064 kb on + strand, within GFF6030 at 91.064 kb on + strand, within GFF6030 at 91.064 kb on + strand, within GFF6030 at 91.064 kb on + strand, within GFF6030 at 91.065 kb on - strand, within GFF6030 at 91.065 kb on - strand, within GFF6030 at 91.065 kb on - strand, within GFF6030 at 91.464 kb on + strand at 91.465 kb on - strand at 91.465 kb on - strand at 91.465 kb on - strand at 91.530 kb on + strand, within GFF6031 at 91.530 kb on + strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 88,716 + GFF6028 0.12 -1.3 89,022 + GFF6028 0.41 +0.1 89,022 + GFF6028 0.41 +0.4 89,023 - GFF6028 0.41 -0.7 89,023 - GFF6028 0.41 +1.9 89,023 - GFF6028 0.41 +0.6 89,023 - GFF6028 0.41 +0.5 89,061 + GFF6028 0.44 -1.3 89,061 + GFF6028 0.44 +0.1 89,062 - GFF6028 0.44 +0.0 89,084 + GFF6028 0.46 -0.1 89,084 + GFF6028 0.46 +0.9 89,084 + GFF6028 0.46 -0.7 89,338 - GFF6028 0.70 +0.2 89,338 - GFF6028 0.70 -0.1 89,338 - GFF6028 0.70 -0.4 89,338 - GFF6028 0.70 +0.1 89,367 + GFF6028 0.73 -0.0 89,367 + GFF6028 0.73 -1.1 89,367 + GFF6028 0.73 +1.2 89,367 + GFF6028 0.73 -0.7 89,367 + GFF6028 0.73 +1.2 89,367 + GFF6028 0.73 +0.0 89,367 + GFF6028 0.73 -0.4 89,367 + GFF6028 0.73 -0.2 89,367 + GFF6028 0.73 -0.6 89,367 + GFF6028 0.73 -1.7 89,367 + GFF6028 0.73 +0.1 89,367 + GFF6028 0.73 -1.5 89,367 + GFF6028 0.73 +1.6 89,367 + GFF6028 0.73 -0.4 89,368 - GFF6028 0.73 -1.5 89,368 - GFF6028 0.73 +0.6 89,368 - GFF6028 0.73 -0.6 89,368 - GFF6028 0.73 -0.4 89,368 - GFF6028 0.73 +0.7 89,368 - GFF6028 0.73 -0.1 89,368 - GFF6028 0.73 +0.0 89,368 - GFF6028 0.73 -0.6 89,368 - GFF6028 0.73 +0.7 89,383 + GFF6028 0.74 +0.4 89,383 + GFF6028 0.74 +0.3 89,384 - GFF6028 0.75 +0.2 89,418 + GFF6028 0.78 -0.1 89,418 + GFF6028 0.78 +0.0 89,419 - GFF6028 0.78 +0.3 89,529 + GFF6028 0.88 -1.3 89,529 + GFF6028 0.88 -0.4 89,529 + GFF6028 0.88 -0.1 89,529 + GFF6028 0.88 -0.4 89,530 - GFF6028 0.88 -0.2 89,530 - GFF6028 0.88 -0.4 89,530 - GFF6028 0.88 -1.3 89,530 - GFF6028 0.88 +0.8 89,530 - GFF6028 0.88 -0.5 89,530 - GFF6028 0.88 +0.3 89,530 - GFF6028 0.88 +0.0 89,530 - GFF6028 0.88 -0.4 89,530 - GFF6028 0.88 +0.1 89,574 + -0.6 89,575 - +0.0 89,575 - +0.1 89,575 - +0.6 89,575 - +0.4 89,665 - -0.7 89,665 - +0.3 89,665 - -0.1 89,668 + +0.8 89,668 + -0.7 89,669 - +1.2 89,669 - +0.0 89,669 - -1.2 89,669 - -0.2 89,794 + GFF6029 0.11 -0.4 89,992 + GFF6029 0.31 +0.2 89,992 + GFF6029 0.31 -0.1 89,992 + GFF6029 0.31 +1.6 89,992 + GFF6029 0.31 -0.7 89,993 - GFF6029 0.32 -0.5 89,993 - GFF6029 0.32 -0.8 90,128 - GFF6029 0.45 -1.3 90,157 + GFF6029 0.48 -0.0 90,158 - GFF6029 0.48 -1.1 90,158 - GFF6029 0.48 -0.6 90,158 - GFF6029 0.48 -0.4 90,244 + GFF6029 0.57 -0.9 90,244 + GFF6029 0.57 -0.3 90,245 - GFF6029 0.57 -0.7 90,245 - GFF6029 0.57 -0.7 90,269 + GFF6029 0.60 -0.5 90,269 + GFF6029 0.60 -0.6 90,269 + GFF6029 0.60 -1.5 90,269 + GFF6029 0.60 -0.7 90,270 - GFF6029 0.60 -1.0 90,270 - GFF6029 0.60 +0.0 90,622 + -0.5 90,622 + -0.2 90,622 + +0.2 90,622 + -0.5 90,677 + +0.6 90,755 + GFF6030 0.12 -1.8 90,755 + GFF6030 0.12 -0.9 90,756 - GFF6030 0.12 +2.2 90,756 - GFF6030 0.12 +0.0 90,756 - GFF6030 0.12 -1.0 90,756 - GFF6030 0.12 +0.2 91,064 + GFF6030 0.51 +1.1 91,064 + GFF6030 0.51 +1.6 91,064 + GFF6030 0.51 -0.6 91,064 + GFF6030 0.51 +0.1 91,065 - GFF6030 0.52 -0.8 91,065 - GFF6030 0.52 -0.4 91,065 - GFF6030 0.52 -0.8 91,464 + -1.0 91,465 - +2.0 91,465 - -0.2 91,465 - +1.4 91,530 + GFF6031 0.11 -0.4 91,530 + GFF6031 0.11 +1.9 91,531 - GFF6031 0.11 +0.8 91,531 - GFF6031 0.11 -0.1 91,531 - GFF6031 0.11 +0.0 91,531 - GFF6031 0.11 -0.1
Or see this region's nucleotide sequence