Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6006 and GFF6007 are separated by 41 nucleotides GFF6007 and GFF6008 are separated by 193 nucleotides GFF6008 and GFF6009 are separated by 13 nucleotides
GFF6006 - BUG/TctC family periplasmic protein, at 64,356 to 65,342
GFF6006
GFF6007 - Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8), at 65,384 to 67,624
GFF6007
GFF6008 - Citrate synthase (si) (EC 2.3.3.1), at 67,818 to 68,603
GFF6008
GFF6009 - Acyl-CoA dehydrogenase STM0857, at 68,617 to 69,780
GFF6009
Position (kb) 65
66
67
68 Strain fitness (log2 ratio) -2
-1
0
1 at 64.453 kb on + strand at 64.454 kb on - strand at 64.586 kb on - strand, within GFF6006 at 64.859 kb on - strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.952 kb on - strand, within GFF6006 at 65.429 kb on + strand at 65.430 kb on - strand at 65.577 kb on + strand at 65.577 kb on + strand at 65.577 kb on + strand at 65.578 kb on - strand at 65.578 kb on - strand at 65.661 kb on + strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.932 kb on + strand, within GFF6007 at 66.414 kb on + strand, within GFF6007 at 66.415 kb on - strand, within GFF6007 at 66.456 kb on + strand, within GFF6007 at 66.672 kb on + strand, within GFF6007 at 66.672 kb on + strand, within GFF6007 at 66.673 kb on - strand, within GFF6007 at 66.673 kb on - strand, within GFF6007 at 66.673 kb on - strand, within GFF6007 at 66.673 kb on - strand, within GFF6007 at 66.877 kb on + strand, within GFF6007 at 66.877 kb on + strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.878 kb on - strand, within GFF6007 at 66.948 kb on + strand, within GFF6007 at 67.666 kb on - strand at 68.061 kb on + strand, within GFF6008 at 68.062 kb on - strand, within GFF6008 at 68.062 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008 at 68.350 kb on - strand, within GFF6008
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 64,453 + +1.0 64,454 - +1.3 64,586 - GFF6006 0.23 -2.3 64,859 - GFF6006 0.51 -0.6 64,951 + GFF6006 0.60 -0.7 64,951 + GFF6006 0.60 -1.0 64,951 + GFF6006 0.60 +1.6 64,952 - GFF6006 0.60 -1.0 65,429 + -0.3 65,430 - +0.4 65,577 + -0.8 65,577 + -0.6 65,577 + +0.6 65,578 - -0.5 65,578 - -0.2 65,661 + GFF6007 0.12 -0.1 65,776 - GFF6007 0.17 +0.4 65,776 - GFF6007 0.17 -1.1 65,776 - GFF6007 0.17 -0.6 65,932 + GFF6007 0.24 -1.2 66,414 + GFF6007 0.46 -0.0 66,415 - GFF6007 0.46 +0.4 66,456 + GFF6007 0.48 -0.4 66,672 + GFF6007 0.57 -0.9 66,672 + GFF6007 0.57 -0.2 66,673 - GFF6007 0.58 -1.0 66,673 - GFF6007 0.58 +0.5 66,673 - GFF6007 0.58 -0.4 66,673 - GFF6007 0.58 -0.4 66,877 + GFF6007 0.67 +0.3 66,877 + GFF6007 0.67 -1.1 66,878 - GFF6007 0.67 -0.5 66,878 - GFF6007 0.67 -1.2 66,878 - GFF6007 0.67 +0.9 66,948 + GFF6007 0.70 +0.4 67,666 - +0.0 68,061 + GFF6008 0.31 -1.4 68,062 - GFF6008 0.31 +0.4 68,062 - GFF6008 0.31 -0.4 68,350 - GFF6008 0.68 +1.1 68,350 - GFF6008 0.68 -0.7 68,350 - GFF6008 0.68 -0.8
Or see this region's nucleotide sequence