Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6005 and GFF6006 are separated by 3 nucleotides GFF6006 and GFF6007 are separated by 41 nucleotides
GFF6005 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 62,847 to 64,352
GFF6005
GFF6006 - BUG/TctC family periplasmic protein, at 64,356 to 65,342
GFF6006
GFF6007 - Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8), at 65,384 to 67,624
GFF6007
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-2
-1
0
1 at 63.440 kb on - strand, within GFF6005 at 63.440 kb on - strand, within GFF6005 at 63.455 kb on - strand, within GFF6005 at 63.532 kb on + strand, within GFF6005 at 63.532 kb on + strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.533 kb on - strand, within GFF6005 at 63.538 kb on + strand, within GFF6005 at 63.539 kb on - strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.601 kb on + strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.602 kb on - strand, within GFF6005 at 63.766 kb on + strand, within GFF6005 at 63.766 kb on + strand, within GFF6005 at 64.255 kb on + strand at 64.255 kb on + strand at 64.255 kb on + strand at 64.255 kb on + strand at 64.256 kb on - strand at 64.357 kb on + strand at 64.453 kb on + strand at 64.454 kb on - strand at 64.586 kb on - strand, within GFF6006 at 64.859 kb on - strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.951 kb on + strand, within GFF6006 at 64.952 kb on - strand, within GFF6006 at 65.429 kb on + strand at 65.430 kb on - strand at 65.577 kb on + strand at 65.577 kb on + strand at 65.577 kb on + strand at 65.578 kb on - strand at 65.578 kb on - strand at 65.661 kb on + strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.776 kb on - strand, within GFF6007 at 65.932 kb on + strand, within GFF6007
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 63,440 - GFF6005 0.39 -0.7 63,440 - GFF6005 0.39 -0.6 63,455 - GFF6005 0.40 +0.3 63,532 + GFF6005 0.45 +0.9 63,532 + GFF6005 0.45 +0.8 63,533 - GFF6005 0.46 +1.1 63,533 - GFF6005 0.46 -2.1 63,533 - GFF6005 0.46 -0.0 63,538 + GFF6005 0.46 -0.6 63,539 - GFF6005 0.46 -0.4 63,601 + GFF6005 0.50 -0.6 63,601 + GFF6005 0.50 -0.1 63,601 + GFF6005 0.50 -0.2 63,601 + GFF6005 0.50 -0.8 63,601 + GFF6005 0.50 -0.1 63,601 + GFF6005 0.50 +0.5 63,601 + GFF6005 0.50 -0.8 63,601 + GFF6005 0.50 -0.1 63,602 - GFF6005 0.50 -0.2 63,602 - GFF6005 0.50 +0.3 63,602 - GFF6005 0.50 +0.1 63,602 - GFF6005 0.50 +0.0 63,602 - GFF6005 0.50 +0.1 63,602 - GFF6005 0.50 -0.0 63,766 + GFF6005 0.61 -0.6 63,766 + GFF6005 0.61 -1.2 64,255 + -1.0 64,255 + +1.1 64,255 + +0.0 64,255 + -0.1 64,256 - +0.4 64,357 + -1.8 64,453 + +1.0 64,454 - +1.3 64,586 - GFF6006 0.23 -2.3 64,859 - GFF6006 0.51 -0.6 64,951 + GFF6006 0.60 -0.7 64,951 + GFF6006 0.60 -1.0 64,951 + GFF6006 0.60 +1.6 64,952 - GFF6006 0.60 -1.0 65,429 + -0.3 65,430 - +0.4 65,577 + -0.8 65,577 + -0.6 65,577 + +0.6 65,578 - -0.5 65,578 - -0.2 65,661 + GFF6007 0.12 -0.1 65,776 - GFF6007 0.17 +0.4 65,776 - GFF6007 0.17 -1.1 65,776 - GFF6007 0.17 -0.6 65,932 + GFF6007 0.24 -1.2
Or see this region's nucleotide sequence