Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5942 and GFF5943 overlap by 4 nucleotides GFF5943 and GFF5944 are separated by 27 nucleotides GFF5944 and GFF5945 are separated by 24 nucleotides
GFF5942 - hypothetical protein, at 162,089 to 164,233
GFF5942
GFF5943 - hypothetical protein, at 164,230 to 165,393
GFF5943
GFF5944 - Adenylosuccinate lyase (EC 4.3.2.2), at 165,421 to 166,170
GFF5944
GFF5945 - Membrane-bound lytic murein transglycosylase A, at 166,195 to 167,796
GFF5945
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1
2 at 163.401 kb on + strand, within GFF5942 at 163.401 kb on + strand, within GFF5942 at 163.402 kb on - strand, within GFF5942 at 163.402 kb on - strand, within GFF5942 at 163.743 kb on + strand, within GFF5942 at 163.788 kb on + strand, within GFF5942 at 163.788 kb on + strand, within GFF5942 at 164.211 kb on + strand at 164.519 kb on + strand, within GFF5943 at 164.520 kb on - strand, within GFF5943 at 165.414 kb on + strand at 165.415 kb on - strand at 165.415 kb on - strand at 165.415 kb on - strand at 165.428 kb on + strand at 165.429 kb on - strand at 165.603 kb on - strand, within GFF5944 at 165.879 kb on - strand, within GFF5944 at 165.890 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.983 kb on + strand, within GFF5944 at 165.984 kb on - strand, within GFF5944 at 165.984 kb on - strand, within GFF5944 at 166.076 kb on + strand, within GFF5944 at 166.076 kb on + strand, within GFF5944
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 163,401 + GFF5942 0.61 -0.3 163,401 + GFF5942 0.61 -1.3 163,402 - GFF5942 0.61 +0.4 163,402 - GFF5942 0.61 -0.3 163,743 + GFF5942 0.77 +0.4 163,788 + GFF5942 0.79 +0.6 163,788 + GFF5942 0.79 -0.2 164,211 + +1.8 164,519 + GFF5943 0.25 -1.4 164,520 - GFF5943 0.25 -1.0 165,414 + -1.2 165,415 - -1.2 165,415 - -2.5 165,415 - -1.5 165,428 + +0.4 165,429 - +0.5 165,603 - GFF5944 0.24 -0.1 165,879 - GFF5944 0.61 +1.4 165,890 + GFF5944 0.63 +0.6 165,983 + GFF5944 0.75 -1.2 165,983 + GFF5944 0.75 -0.3 165,983 + GFF5944 0.75 -0.4 165,984 - GFF5944 0.75 -0.0 165,984 - GFF5944 0.75 -1.1 166,076 + GFF5944 0.87 -0.0 166,076 + GFF5944 0.87 -0.6
Or see this region's nucleotide sequence