Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5927 and GFF5928 overlap by 4 nucleotides GFF5928 and GFF5929 are separated by 25 nucleotides GFF5929 and GFF5930 are separated by 21 nucleotides
GFF5927 - 3-oxoacyl-[ACP] synthase, at 142,200 to 143,237
GFF5927
GFF5928 - hypothetical protein, at 143,234 to 144,346
GFF5928
GFF5929 - Mll2356 protein, at 144,372 to 144,854
GFF5929
GFF5930 - VgrG protein, at 144,876 to 147,254
GFF5930
Position (kb) 143
144
145 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 142.447 kb on + strand, within GFF5927 at 142.447 kb on + strand, within GFF5927 at 142.448 kb on - strand, within GFF5927 at 143.620 kb on - strand, within GFF5928 at 143.620 kb on - strand, within GFF5928 at 143.679 kb on + strand, within GFF5928 at 143.679 kb on + strand, within GFF5928 at 143.745 kb on + strand, within GFF5928 at 143.745 kb on + strand, within GFF5928 at 143.745 kb on + strand, within GFF5928 at 143.745 kb on + strand, within GFF5928 at 143.746 kb on - strand, within GFF5928 at 143.749 kb on + strand, within GFF5928 at 143.865 kb on + strand, within GFF5928 at 143.865 kb on + strand, within GFF5928 at 143.865 kb on + strand, within GFF5928 at 143.865 kb on + strand, within GFF5928 at 143.866 kb on - strand, within GFF5928 at 143.866 kb on - strand, within GFF5928 at 143.866 kb on - strand, within GFF5928 at 143.866 kb on - strand, within GFF5928 at 143.926 kb on - strand, within GFF5928 at 144.022 kb on - strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.132 kb on + strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.133 kb on - strand, within GFF5928 at 144.433 kb on + strand, within GFF5929 at 144.433 kb on + strand, within GFF5929 at 144.434 kb on - strand, within GFF5929 at 144.860 kb on + strand at 144.860 kb on + strand at 144.861 kb on - strand at 145.310 kb on + strand, within GFF5930 at 145.311 kb on - strand, within GFF5930
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 142,447 + GFF5927 0.24 -2.9 142,447 + GFF5927 0.24 -1.0 142,448 - GFF5927 0.24 -0.2 143,620 - GFF5928 0.35 +0.3 143,620 - GFF5928 0.35 +1.1 143,679 + GFF5928 0.40 -0.8 143,679 + GFF5928 0.40 -0.4 143,745 + GFF5928 0.46 -0.6 143,745 + GFF5928 0.46 -1.4 143,745 + GFF5928 0.46 +0.3 143,745 + GFF5928 0.46 -0.3 143,746 - GFF5928 0.46 +1.1 143,749 + GFF5928 0.46 -0.5 143,865 + GFF5928 0.57 -0.5 143,865 + GFF5928 0.57 +0.2 143,865 + GFF5928 0.57 -0.3 143,865 + GFF5928 0.57 +0.6 143,866 - GFF5928 0.57 +0.2 143,866 - GFF5928 0.57 -0.4 143,866 - GFF5928 0.57 -0.4 143,866 - GFF5928 0.57 -0.6 143,926 - GFF5928 0.62 -1.3 144,022 - GFF5928 0.71 -0.2 144,132 + GFF5928 0.81 +0.1 144,132 + GFF5928 0.81 -0.8 144,132 + GFF5928 0.81 -0.5 144,132 + GFF5928 0.81 -0.9 144,133 - GFF5928 0.81 -1.4 144,133 - GFF5928 0.81 +1.1 144,133 - GFF5928 0.81 +0.0 144,433 + GFF5929 0.13 -0.2 144,433 + GFF5929 0.13 +1.0 144,434 - GFF5929 0.13 -0.4 144,860 + +0.2 144,860 + -0.5 144,861 - +0.6 145,310 + GFF5930 0.18 +0.2 145,311 - GFF5930 0.18 -0.1
Or see this region's nucleotide sequence