Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5807 and GFF5808 are separated by 85 nucleotides GFF5808 and GFF5809 are separated by 190 nucleotides
GFF5807 - hypothetical protein, at 182,438 to 183,685
GFF5807
GFF5808 - Putative oxidoreductase, at 183,771 to 184,781
GFF5808
GFF5809 - Outer membrane factor (OMF) lipoprotein associated wth MdtABC efflux system, at 184,972 to 186,465
GFF5809
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 182.906 kb on + strand, within GFF5807 at 182.907 kb on - strand, within GFF5807 at 183.039 kb on - strand, within GFF5807 at 183.352 kb on + strand, within GFF5807 at 183.352 kb on + strand, within GFF5807 at 183.352 kb on + strand, within GFF5807 at 183.352 kb on + strand, within GFF5807 at 183.352 kb on + strand, within GFF5807 at 183.353 kb on - strand, within GFF5807 at 183.353 kb on - strand, within GFF5807 at 183.623 kb on + strand at 183.623 kb on + strand at 183.630 kb on - strand at 183.650 kb on + strand at 183.650 kb on + strand at 183.651 kb on - strand at 183.651 kb on - strand at 183.674 kb on - strand at 183.710 kb on + strand at 183.710 kb on + strand at 183.710 kb on + strand at 183.710 kb on + strand at 183.710 kb on + strand at 183.711 kb on - strand at 183.782 kb on + strand at 183.933 kb on + strand, within GFF5808 at 184.156 kb on - strand, within GFF5808 at 184.242 kb on + strand, within GFF5808 at 184.242 kb on + strand, within GFF5808 at 184.256 kb on + strand, within GFF5808 at 184.320 kb on + strand, within GFF5808 at 184.320 kb on + strand, within GFF5808 at 184.321 kb on - strand, within GFF5808 at 184.342 kb on - strand, within GFF5808 at 184.416 kb on + strand, within GFF5808 at 184.417 kb on - strand, within GFF5808 at 184.452 kb on + strand, within GFF5808 at 184.452 kb on + strand, within GFF5808 at 184.452 kb on + strand, within GFF5808 at 184.515 kb on + strand, within GFF5808 at 184.515 kb on + strand, within GFF5808 at 184.515 kb on + strand, within GFF5808 at 184.515 kb on + strand, within GFF5808 at 184.516 kb on - strand, within GFF5808 at 184.704 kb on + strand at 184.704 kb on + strand at 184.705 kb on - strand at 184.925 kb on + strand at 184.957 kb on + strand at 184.957 kb on + strand at 184.958 kb on - strand at 184.958 kb on - strand at 184.958 kb on - strand at 184.997 kb on + strand at 185.059 kb on + strand at 185.059 kb on + strand at 185.515 kb on + strand, within GFF5809 at 185.515 kb on + strand, within GFF5809 at 185.515 kb on + strand, within GFF5809 at 185.516 kb on - strand, within GFF5809 at 185.575 kb on + strand, within GFF5809 at 185.609 kb on - strand, within GFF5809
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 182,906 + GFF5807 0.38 -2.0 182,907 - GFF5807 0.38 -0.3 183,039 - GFF5807 0.48 -0.4 183,352 + GFF5807 0.73 -1.2 183,352 + GFF5807 0.73 +0.1 183,352 + GFF5807 0.73 +0.3 183,352 + GFF5807 0.73 -0.0 183,352 + GFF5807 0.73 -0.0 183,353 - GFF5807 0.73 -0.6 183,353 - GFF5807 0.73 -0.1 183,623 + -0.2 183,623 + -0.9 183,630 - -1.1 183,650 + +1.5 183,650 + +1.3 183,651 - +0.7 183,651 - -0.3 183,674 - -0.2 183,710 + +0.2 183,710 + -0.5 183,710 + +0.4 183,710 + -0.2 183,710 + -0.3 183,711 - -0.4 183,782 + -0.6 183,933 + GFF5808 0.16 -1.0 184,156 - GFF5808 0.38 +0.4 184,242 + GFF5808 0.47 +0.9 184,242 + GFF5808 0.47 -1.0 184,256 + GFF5808 0.48 -0.7 184,320 + GFF5808 0.54 -1.3 184,320 + GFF5808 0.54 +0.1 184,321 - GFF5808 0.54 +2.1 184,342 - GFF5808 0.56 -0.7 184,416 + GFF5808 0.64 +0.5 184,417 - GFF5808 0.64 +0.1 184,452 + GFF5808 0.67 -0.0 184,452 + GFF5808 0.67 -0.5 184,452 + GFF5808 0.67 -0.5 184,515 + GFF5808 0.74 -0.5 184,515 + GFF5808 0.74 -0.1 184,515 + GFF5808 0.74 -1.0 184,515 + GFF5808 0.74 +0.6 184,516 - GFF5808 0.74 -1.0 184,704 + -0.2 184,704 + -0.9 184,705 - -0.2 184,925 + -2.0 184,957 + -1.3 184,957 + +0.2 184,958 - -0.8 184,958 - +0.8 184,958 - -0.4 184,997 + -0.7 185,059 + -0.1 185,059 + -0.6 185,515 + GFF5809 0.36 +0.3 185,515 + GFF5809 0.36 -0.8 185,515 + GFF5809 0.36 +0.7 185,516 - GFF5809 0.36 +0.4 185,575 + GFF5809 0.40 -0.3 185,609 - GFF5809 0.43 +1.0
Or see this region's nucleotide sequence