Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5668 and GFF5669 overlap by 4 nucleotides GFF5669 and GFF5670 overlap by 4 nucleotides GFF5670 and GFF5671 are separated by 10 nucleotides GFF5671 and GFF5672 are separated by 74 nucleotides GFF5672 and GFF5673 overlap by 4 nucleotides
GFF5668 - Uncharacterized MFS-type transporter, at 43,891 to 45,402
GFF5668
GFF5669 - hypothetical protein, at 45,399 to 45,677
GFF5669
GFF5670 - Protein SlyX, at 45,674 to 45,865
GFF5670
GFF5671 - FIG01964566: Predicted membrane protein, hemolysin III homolog, at 45,876 to 46,562
GFF5671
GFF5672 - Dna binding response regulator PrrA (RegA), at 46,637 to 47,182
GFF5672
GFF5673 - Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-), at 47,179 to 48,495
GFF5673
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 45.265 kb on + strand at 45.265 kb on + strand at 45.265 kb on + strand at 45.265 kb on + strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.266 kb on - strand at 45.354 kb on + strand at 45.355 kb on - strand at 45.355 kb on - strand at 45.590 kb on - strand, within GFF5669 at 45.701 kb on + strand, within GFF5670 at 45.702 kb on - strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.740 kb on + strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.741 kb on - strand, within GFF5670 at 45.849 kb on + strand at 45.849 kb on + strand at 45.849 kb on + strand at 45.850 kb on - strand at 45.850 kb on - strand at 45.860 kb on + strand at 45.860 kb on + strand at 45.860 kb on + strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.861 kb on - strand at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.959 kb on - strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.984 kb on + strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 45.985 kb on - strand, within GFF5671 at 46.001 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.003 kb on + strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.004 kb on - strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.057 kb on + strand, within GFF5671 at 46.058 kb on - strand, within GFF5671 at 46.058 kb on - strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.102 kb on + strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.103 kb on - strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.240 kb on + strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.241 kb on - strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.261 kb on + strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.262 kb on - strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.324 kb on + strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.325 kb on - strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.342 kb on + strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.343 kb on - strand, within GFF5671 at 46.417 kb on + strand, within GFF5671 at 46.417 kb on + strand, within GFF5671 at 46.418 kb on - strand, within GFF5671 at 46.418 kb on - strand, within GFF5671 at 46.630 kb on + strand at 46.630 kb on + strand at 46.630 kb on + strand at 46.631 kb on - strand at 46.631 kb on - strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.635 kb on + strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.636 kb on - strand at 46.638 kb on + strand at 46.638 kb on + strand at 46.638 kb on + strand at 46.638 kb on + strand at 46.638 kb on + strand at 46.638 kb on + strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.639 kb on - strand at 46.675 kb on + strand at 46.675 kb on + strand at 46.676 kb on - strand at 46.676 kb on - strand at 46.747 kb on + strand, within GFF5672 at 46.866 kb on + strand, within GFF5672 at 46.866 kb on + strand, within GFF5672 at 46.867 kb on - strand, within GFF5672 at 46.867 kb on - strand, within GFF5672 at 47.023 kb on - strand, within GFF5672 at 47.088 kb on + strand, within GFF5672 at 47.088 kb on + strand, within GFF5672 at 47.088 kb on + strand, within GFF5672 at 47.420 kb on + strand, within GFF5673 at 47.421 kb on - strand, within GFF5673
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 45,265 + -0.9 45,265 + +0.5 45,265 + +0.4 45,265 + +0.0 45,266 - -0.9 45,266 - -1.1 45,266 - -0.2 45,266 - -0.6 45,266 - +1.0 45,266 - -0.8 45,354 + -0.2 45,355 - -0.3 45,355 - -0.7 45,590 - GFF5669 0.68 -0.3 45,701 + GFF5670 0.14 +0.2 45,702 - GFF5670 0.15 +0.6 45,740 + GFF5670 0.34 +0.3 45,740 + GFF5670 0.34 -1.1 45,740 + GFF5670 0.34 +0.5 45,740 + GFF5670 0.34 +0.1 45,740 + GFF5670 0.34 -0.4 45,741 - GFF5670 0.35 -0.2 45,741 - GFF5670 0.35 -0.9 45,741 - GFF5670 0.35 -0.7 45,741 - GFF5670 0.35 +0.6 45,741 - GFF5670 0.35 +0.1 45,849 + +0.2 45,849 + -0.9 45,849 + +1.4 45,850 - -1.6 45,850 - -1.8 45,860 + -0.1 45,860 + +1.3 45,860 + -0.3 45,861 - +0.1 45,861 - +0.7 45,861 - +0.0 45,861 - -1.0 45,959 - GFF5671 0.12 -0.0 45,959 - GFF5671 0.12 +2.3 45,959 - GFF5671 0.12 +0.4 45,959 - GFF5671 0.12 -1.4 45,959 - GFF5671 0.12 -1.6 45,959 - GFF5671 0.12 -1.0 45,984 + GFF5671 0.16 +0.0 45,984 + GFF5671 0.16 -1.0 45,984 + GFF5671 0.16 -0.9 45,984 + GFF5671 0.16 -1.6 45,984 + GFF5671 0.16 +0.7 45,985 - GFF5671 0.16 -0.0 45,985 - GFF5671 0.16 +0.6 45,985 - GFF5671 0.16 +0.1 45,985 - GFF5671 0.16 -0.7 46,001 + GFF5671 0.18 -0.7 46,003 + GFF5671 0.18 -0.2 46,003 + GFF5671 0.18 -0.4 46,003 + GFF5671 0.18 +0.2 46,003 + GFF5671 0.18 +0.2 46,003 + GFF5671 0.18 +0.7 46,003 + GFF5671 0.18 +0.3 46,003 + GFF5671 0.18 +0.3 46,003 + GFF5671 0.18 +0.8 46,003 + GFF5671 0.18 -0.5 46,003 + GFF5671 0.18 -0.9 46,003 + GFF5671 0.18 -0.8 46,003 + GFF5671 0.18 +0.7 46,004 - GFF5671 0.19 +0.2 46,004 - GFF5671 0.19 -1.3 46,004 - GFF5671 0.19 -0.0 46,004 - GFF5671 0.19 +0.3 46,004 - GFF5671 0.19 +0.1 46,004 - GFF5671 0.19 +0.3 46,004 - GFF5671 0.19 +0.2 46,004 - GFF5671 0.19 +1.0 46,004 - GFF5671 0.19 +0.0 46,057 + GFF5671 0.26 -0.2 46,057 + GFF5671 0.26 -0.3 46,057 + GFF5671 0.26 +1.1 46,057 + GFF5671 0.26 -0.5 46,058 - GFF5671 0.26 -0.4 46,058 - GFF5671 0.26 -1.3 46,102 + GFF5671 0.33 -0.0 46,102 + GFF5671 0.33 -0.5 46,102 + GFF5671 0.33 -0.1 46,102 + GFF5671 0.33 -0.6 46,102 + GFF5671 0.33 -0.9 46,102 + GFF5671 0.33 +0.0 46,102 + GFF5671 0.33 +0.1 46,103 - GFF5671 0.33 +0.4 46,103 - GFF5671 0.33 -0.1 46,103 - GFF5671 0.33 -0.5 46,103 - GFF5671 0.33 +0.1 46,103 - GFF5671 0.33 -0.3 46,103 - GFF5671 0.33 +0.0 46,103 - GFF5671 0.33 +1.1 46,103 - GFF5671 0.33 -1.0 46,103 - GFF5671 0.33 +1.0 46,240 + GFF5671 0.53 -0.3 46,240 + GFF5671 0.53 -0.1 46,240 + GFF5671 0.53 +0.3 46,240 + GFF5671 0.53 -1.1 46,240 + GFF5671 0.53 -0.2 46,240 + GFF5671 0.53 -0.2 46,240 + GFF5671 0.53 +0.3 46,240 + GFF5671 0.53 +1.3 46,240 + GFF5671 0.53 +1.1 46,240 + GFF5671 0.53 -1.1 46,240 + GFF5671 0.53 -0.5 46,241 - GFF5671 0.53 -0.2 46,241 - GFF5671 0.53 -0.3 46,241 - GFF5671 0.53 -0.2 46,241 - GFF5671 0.53 -0.2 46,241 - GFF5671 0.53 +0.0 46,241 - GFF5671 0.53 -1.1 46,241 - GFF5671 0.53 -1.1 46,241 - GFF5671 0.53 -0.6 46,241 - GFF5671 0.53 +2.3 46,241 - GFF5671 0.53 -0.3 46,261 + GFF5671 0.56 +0.5 46,261 + GFF5671 0.56 +0.3 46,261 + GFF5671 0.56 +2.2 46,261 + GFF5671 0.56 +0.4 46,261 + GFF5671 0.56 -0.7 46,262 - GFF5671 0.56 +0.5 46,262 - GFF5671 0.56 +0.6 46,262 - GFF5671 0.56 -0.2 46,262 - GFF5671 0.56 -0.3 46,262 - GFF5671 0.56 +0.2 46,262 - GFF5671 0.56 -1.1 46,262 - GFF5671 0.56 +0.6 46,262 - GFF5671 0.56 -0.5 46,262 - GFF5671 0.56 -0.8 46,324 + GFF5671 0.65 +0.0 46,324 + GFF5671 0.65 -0.8 46,324 + GFF5671 0.65 +0.4 46,325 - GFF5671 0.65 +0.7 46,325 - GFF5671 0.65 -0.3 46,325 - GFF5671 0.65 +2.2 46,325 - GFF5671 0.65 +0.7 46,342 + GFF5671 0.68 +0.4 46,342 + GFF5671 0.68 +0.1 46,342 + GFF5671 0.68 +0.0 46,342 + GFF5671 0.68 -0.7 46,342 + GFF5671 0.68 -0.7 46,343 - GFF5671 0.68 -0.3 46,343 - GFF5671 0.68 +0.0 46,343 - GFF5671 0.68 -0.4 46,343 - GFF5671 0.68 -0.1 46,417 + GFF5671 0.79 +0.2 46,417 + GFF5671 0.79 +0.2 46,418 - GFF5671 0.79 -0.3 46,418 - GFF5671 0.79 -0.2 46,630 + +0.5 46,630 + +0.2 46,630 + +0.0 46,631 - +2.7 46,631 - -0.6 46,635 + +1.7 46,635 + -1.3 46,635 + +0.5 46,635 + -0.1 46,635 + +0.9 46,635 + -0.6 46,635 + -0.2 46,636 - +0.1 46,636 - -0.1 46,636 - -1.3 46,636 - +0.1 46,636 - -0.0 46,636 - -1.6 46,636 - +1.0 46,638 + +0.8 46,638 + -1.7 46,638 + +2.1 46,638 + -0.3 46,638 + -0.5 46,638 + -0.1 46,639 - -0.4 46,639 - -0.8 46,639 - -0.1 46,639 - -0.1 46,639 - -1.0 46,639 - +0.0 46,639 - -0.3 46,639 - -1.0 46,639 - -0.2 46,639 - +0.4 46,639 - +0.4 46,639 - -0.9 46,675 + +0.1 46,675 + -0.2 46,676 - +1.7 46,676 - -0.7 46,747 + GFF5672 0.20 -0.6 46,866 + GFF5672 0.42 +0.2 46,866 + GFF5672 0.42 -0.5 46,867 - GFF5672 0.42 +0.4 46,867 - GFF5672 0.42 -0.4 47,023 - GFF5672 0.71 +0.0 47,088 + GFF5672 0.83 -1.3 47,088 + GFF5672 0.83 -0.8 47,088 + GFF5672 0.83 +0.0 47,420 + GFF5673 0.18 -0.0 47,421 - GFF5673 0.18 +0.2
Or see this region's nucleotide sequence