Strain Fitness in Variovorax sp. SCN45 around GFF5545

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5543 and GFF5544 are separated by 2 nucleotidesGFF5544 and GFF5545 are separated by 10 nucleotidesGFF5545 and GFF5546 are separated by 45 nucleotidesGFF5546 and GFF5547 overlap by 4 nucleotides GFF5543 - FIG015373: Membrane protein, at 171,381 to 172,118 GFF5543 GFF5544 - FIG001614: Membrane protein, at 172,121 to 173,077 GFF5544 GFF5545 - Pyrrolidone-carboxylate peptidase (EC 3.4.19.3), at 173,088 to 173,771 GFF5545 GFF5546 - Lactam utilization protein LamB, at 173,817 to 174,584 GFF5546 GFF5547 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) / Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 174,581 to 176,173 GFF5547 Position (kb) 173 174Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.145 kb on + strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.146 kb on - strandat 172.217 kb on + strand, within GFF5544at 172.217 kb on + strand, within GFF5544at 172.217 kb on + strand, within GFF5544at 172.217 kb on + strand, within GFF5544at 172.217 kb on + strand, within GFF5544at 172.218 kb on - strand, within GFF5544at 172.218 kb on - strand, within GFF5544at 172.218 kb on - strand, within GFF5544at 172.218 kb on - strand, within GFF5544at 172.244 kb on + strand, within GFF5544at 172.244 kb on + strand, within GFF5544at 172.244 kb on + strand, within GFF5544at 172.244 kb on + strand, within GFF5544at 172.244 kb on + strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.245 kb on - strand, within GFF5544at 172.376 kb on + strand, within GFF5544at 172.376 kb on + strand, within GFF5544at 172.376 kb on + strand, within GFF5544at 172.376 kb on + strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.377 kb on - strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.724 kb on + strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.725 kb on - strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.763 kb on + strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.764 kb on - strand, within GFF5544at 172.865 kb on + strand, within GFF5544at 172.865 kb on + strand, within GFF5544at 172.865 kb on + strand, within GFF5544at 172.865 kb on + strand, within GFF5544at 172.865 kb on + strand, within GFF5544at 172.866 kb on - strand, within GFF5544at 172.866 kb on - strand, within GFF5544at 172.913 kb on + strand, within GFF5544at 172.914 kb on - strand, within GFF5544at 173.013 kb on + strandat 173.013 kb on + strandat 173.013 kb on + strandat 173.014 kb on - strandat 173.014 kb on - strandat 173.014 kb on - strandat 173.057 kb on + strandat 173.057 kb on + strandat 173.057 kb on + strandat 173.058 kb on - strandat 173.058 kb on - strandat 173.406 kb on + strand, within GFF5545at 173.451 kb on + strand, within GFF5545at 173.451 kb on + strand, within GFF5545at 173.451 kb on + strand, within GFF5545at 173.452 kb on - strand, within GFF5545at 173.452 kb on - strand, within GFF5545at 173.556 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.559 kb on + strand, within GFF5545at 173.560 kb on - strand, within GFF5545at 173.560 kb on - strand, within GFF5545at 173.560 kb on - strand, within GFF5545at 173.560 kb on - strand, within GFF5545at 173.631 kb on + strand, within GFF5545at 173.632 kb on - strand, within GFF5545at 173.632 kb on - strand, within GFF5545at 173.632 kb on - strand, within GFF5545at 173.632 kb on - strand, within GFF5545at 173.632 kb on - strand, within GFF5545at 174.009 kb on + strand, within GFF5546at 174.010 kb on - strand, within GFF5546at 174.081 kb on + strand, within GFF5546at 174.081 kb on + strand, within GFF5546at 174.081 kb on + strand, within GFF5546at 174.081 kb on + strand, within GFF5546at 174.082 kb on - strand, within GFF5546at 174.082 kb on - strand, within GFF5546at 174.082 kb on - strand, within GFF5546at 174.136 kb on - strand, within GFF5546at 174.136 kb on - strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.159 kb on + strand, within GFF5546at 174.160 kb on - strand, within GFF5546at 174.160 kb on - strand, within GFF5546at 174.160 kb on - strand, within GFF5546at 174.160 kb on - strand, within GFF5546at 174.160 kb on - strand, within GFF5546at 174.325 kb on - strand, within GFF5546at 174.325 kb on - strand, within GFF5546at 174.325 kb on - strand, within GFF5546

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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172,145 + -0.3
172,145 + -1.5
172,145 + -0.4
172,145 + +0.5
172,145 + +2.5
172,145 + -0.3
172,145 + -0.8
172,145 + -0.6
172,145 + +0.0
172,145 + -0.9
172,145 + +0.1
172,145 + -0.7
172,145 + -1.1
172,145 + -1.2
172,145 + +0.4
172,145 + +0.2
172,145 + +1.6
172,145 + -1.1
172,145 + -0.5
172,146 - -0.4
172,146 - +0.2
172,146 - +1.1
172,146 - -1.0
172,146 - +0.1
172,146 - +0.1
172,146 - +0.3
172,146 - -0.5
172,146 - +0.1
172,146 - -0.4
172,146 - +0.3
172,146 - -0.6
172,146 - -1.1
172,146 - +0.4
172,146 - -1.0
172,146 - -0.5
172,146 - -0.8
172,146 - -1.2
172,146 - +0.7
172,146 - +1.4
172,146 - -0.1
172,217 + GFF5544 0.10 +0.4
172,217 + GFF5544 0.10 -1.7
172,217 + GFF5544 0.10 -0.2
172,217 + GFF5544 0.10 -0.2
172,217 + GFF5544 0.10 -1.6
172,218 - GFF5544 0.10 -0.7
172,218 - GFF5544 0.10 +1.4
172,218 - GFF5544 0.10 -1.6
172,218 - GFF5544 0.10 -0.5
172,244 + GFF5544 0.13 +0.2
172,244 + GFF5544 0.13 -0.1
172,244 + GFF5544 0.13 -0.1
172,244 + GFF5544 0.13 -0.4
172,244 + GFF5544 0.13 -0.4
172,245 - GFF5544 0.13 -0.6
172,245 - GFF5544 0.13 -0.2
172,245 - GFF5544 0.13 +0.8
172,245 - GFF5544 0.13 +0.8
172,245 - GFF5544 0.13 -0.2
172,245 - GFF5544 0.13 -0.5
172,376 + GFF5544 0.27 -0.4
172,376 + GFF5544 0.27 -0.6
172,376 + GFF5544 0.27 -0.8
172,376 + GFF5544 0.27 -0.2
172,377 - GFF5544 0.27 +0.5
172,377 - GFF5544 0.27 +0.1
172,377 - GFF5544 0.27 +0.3
172,377 - GFF5544 0.27 -0.4
172,377 - GFF5544 0.27 +0.2
172,377 - GFF5544 0.27 +0.4
172,724 + GFF5544 0.63 +1.9
172,724 + GFF5544 0.63 -0.2
172,724 + GFF5544 0.63 +1.9
172,724 + GFF5544 0.63 +0.1
172,724 + GFF5544 0.63 -0.3
172,724 + GFF5544 0.63 -0.3
172,724 + GFF5544 0.63 -0.3
172,724 + GFF5544 0.63 -0.1
172,724 + GFF5544 0.63 -0.2
172,724 + GFF5544 0.63 -2.0
172,724 + GFF5544 0.63 -0.0
172,724 + GFF5544 0.63 -0.8
172,724 + GFF5544 0.63 -0.6
172,724 + GFF5544 0.63 +1.4
172,724 + GFF5544 0.63 +0.9
172,724 + GFF5544 0.63 -0.4
172,725 - GFF5544 0.63 +1.3
172,725 - GFF5544 0.63 -0.5
172,725 - GFF5544 0.63 +0.7
172,725 - GFF5544 0.63 +0.2
172,725 - GFF5544 0.63 +0.3
172,725 - GFF5544 0.63 -0.2
172,725 - GFF5544 0.63 +0.5
172,725 - GFF5544 0.63 +0.0
172,725 - GFF5544 0.63 +0.5
172,725 - GFF5544 0.63 -0.1
172,725 - GFF5544 0.63 -0.4
172,725 - GFF5544 0.63 +1.1
172,725 - GFF5544 0.63 -1.1
172,725 - GFF5544 0.63 -0.0
172,725 - GFF5544 0.63 -0.1
172,725 - GFF5544 0.63 -0.1
172,725 - GFF5544 0.63 +1.9
172,725 - GFF5544 0.63 +0.7
172,725 - GFF5544 0.63 -0.4
172,725 - GFF5544 0.63 -0.9
172,725 - GFF5544 0.63 +0.4
172,725 - GFF5544 0.63 +1.4
172,725 - GFF5544 0.63 -0.1
172,763 + GFF5544 0.67 -0.8
172,763 + GFF5544 0.67 -0.0
172,763 + GFF5544 0.67 +0.1
172,763 + GFF5544 0.67 +1.2
172,763 + GFF5544 0.67 -0.7
172,763 + GFF5544 0.67 -0.2
172,763 + GFF5544 0.67 -0.2
172,763 + GFF5544 0.67 -0.7
172,763 + GFF5544 0.67 -0.8
172,763 + GFF5544 0.67 +0.7
172,764 - GFF5544 0.67 +0.3
172,764 - GFF5544 0.67 +0.7
172,764 - GFF5544 0.67 +0.3
172,764 - GFF5544 0.67 -0.8
172,764 - GFF5544 0.67 +0.8
172,764 - GFF5544 0.67 -1.8
172,764 - GFF5544 0.67 +0.4
172,764 - GFF5544 0.67 -0.3
172,764 - GFF5544 0.67 +0.2
172,865 + GFF5544 0.78 -0.1
172,865 + GFF5544 0.78 -0.5
172,865 + GFF5544 0.78 -0.3
172,865 + GFF5544 0.78 +0.4
172,865 + GFF5544 0.78 -0.9
172,866 - GFF5544 0.78 +0.4
172,866 - GFF5544 0.78 +0.4
172,913 + GFF5544 0.83 -0.1
172,914 - GFF5544 0.83 +0.2
173,013 + -0.8
173,013 + +0.9
173,013 + -0.0
173,014 - -1.0
173,014 - +0.6
173,014 - +0.0
173,057 + +1.4
173,057 + +0.9
173,057 + -0.5
173,058 - +0.4
173,058 - +0.4
173,406 + GFF5545 0.46 +0.4
173,451 + GFF5545 0.53 -0.1
173,451 + GFF5545 0.53 +0.0
173,451 + GFF5545 0.53 +0.6
173,452 - GFF5545 0.53 +0.1
173,452 - GFF5545 0.53 -0.3
173,556 + GFF5545 0.68 +0.3
173,559 + GFF5545 0.69 -0.3
173,559 + GFF5545 0.69 -0.6
173,559 + GFF5545 0.69 +0.6
173,559 + GFF5545 0.69 +0.2
173,559 + GFF5545 0.69 -0.4
173,559 + GFF5545 0.69 +0.2
173,559 + GFF5545 0.69 -0.2
173,559 + GFF5545 0.69 -0.8
173,560 - GFF5545 0.69 -0.5
173,560 - GFF5545 0.69 +1.0
173,560 - GFF5545 0.69 -0.8
173,560 - GFF5545 0.69 +0.1
173,631 + GFF5545 0.79 +0.6
173,632 - GFF5545 0.80 -0.1
173,632 - GFF5545 0.80 +0.5
173,632 - GFF5545 0.80 +0.4
173,632 - GFF5545 0.80 -0.5
173,632 - GFF5545 0.80 -0.4
174,009 + GFF5546 0.25 -0.4
174,010 - GFF5546 0.25 +0.8
174,081 + GFF5546 0.34 +1.4
174,081 + GFF5546 0.34 +0.1
174,081 + GFF5546 0.34 +0.2
174,081 + GFF5546 0.34 +0.4
174,082 - GFF5546 0.35 -3.1
174,082 - GFF5546 0.35 +0.4
174,082 - GFF5546 0.35 -2.1
174,136 - GFF5546 0.42 -0.2
174,136 - GFF5546 0.42 +0.1
174,159 + GFF5546 0.45 -0.7
174,159 + GFF5546 0.45 -0.5
174,159 + GFF5546 0.45 +0.7
174,159 + GFF5546 0.45 +0.7
174,159 + GFF5546 0.45 -0.4
174,159 + GFF5546 0.45 -1.5
174,159 + GFF5546 0.45 -0.9
174,159 + GFF5546 0.45 -0.2
174,159 + GFF5546 0.45 -0.5
174,159 + GFF5546 0.45 -2.1
174,160 - GFF5546 0.45 -0.9
174,160 - GFF5546 0.45 +0.4
174,160 - GFF5546 0.45 -0.2
174,160 - GFF5546 0.45 +0.9
174,160 - GFF5546 0.45 -0.1
174,325 - GFF5546 0.66 -1.6
174,325 - GFF5546 0.66 -0.1
174,325 - GFF5546 0.66 -0.4

Or see this region's nucleotide sequence