Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5447 and GFF5448 are separated by 7 nucleotides GFF5448 and GFF5449 are separated by 128 nucleotides GFF5449 and GFF5450 are separated by 196 nucleotides GFF5450 and GFF5451 are separated by 31 nucleotides
GFF5447 - Two-component transcriptional response regulator, LuxR family, at 77,920 to 78,546
GFF5447
GFF5448 - Outer membrane lipoprotein omp16 precursor, at 78,554 to 79,120
GFF5448
GFF5449 - Probable lipoprotein YiaD, at 79,249 to 79,905
GFF5449
GFF5450 - hypothetical protein, at 80,102 to 80,242
GFF5450
GFF5451 - Membrane-bound lytic murein transglycosylase A, at 80,274 to 81,392
GFF5451
Position (kb)
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 78.544 kb on + strand at 78.544 kb on + strand at 78.544 kb on + strand at 78.544 kb on + strand at 78.544 kb on + strand at 78.544 kb on + strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 78.545 kb on - strand at 79.116 kb on - strand at 79.138 kb on + strand at 79.138 kb on + strand at 79.138 kb on + strand at 79.138 kb on + strand at 79.138 kb on + strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.139 kb on - strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.142 kb on + strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.143 kb on - strand at 79.173 kb on + strand at 79.174 kb on - strand at 79.174 kb on - strand at 79.397 kb on + strand, within GFF5449 at 79.397 kb on + strand, within GFF5449 at 79.397 kb on + strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.398 kb on - strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.700 kb on + strand, within GFF5449 at 79.701 kb on - strand, within GFF5449 at 79.743 kb on + strand, within GFF5449 at 79.743 kb on + strand, within GFF5449 at 79.744 kb on - strand, within GFF5449 at 79.744 kb on - strand, within GFF5449 at 79.744 kb on - strand, within GFF5449 at 79.821 kb on + strand, within GFF5449 at 79.822 kb on - strand, within GFF5449 at 79.892 kb on - strand at 79.892 kb on - strand at 79.892 kb on - strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.945 kb on + strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.946 kb on - strand at 79.968 kb on + strand at 79.968 kb on + strand at 79.969 kb on - strand at 79.993 kb on + strand at 80.045 kb on - strand at 80.092 kb on + strand at 80.092 kb on + strand at 80.092 kb on + strand at 80.092 kb on + strand at 80.093 kb on - strand at 80.093 kb on - strand at 80.093 kb on - strand at 80.093 kb on - strand at 80.093 kb on - strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.281 kb on + strand at 80.282 kb on - strand at 80.282 kb on - strand at 80.282 kb on - strand at 80.282 kb on - strand at 80.282 kb on - strand at 80.282 kb on - strand at 80.595 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.643 kb on + strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.644 kb on - strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.646 kb on + strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.647 kb on - strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.658 kb on + strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.659 kb on - strand, within GFF5451 at 80.703 kb on + strand, within GFF5451 at 80.703 kb on + strand, within GFF5451 at 80.703 kb on + strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.704 kb on - strand, within GFF5451 at 80.742 kb on + strand, within GFF5451 at 80.742 kb on + strand, within GFF5451 at 80.742 kb on + strand, within GFF5451 at 80.743 kb on - strand, within GFF5451 at 80.887 kb on - strand, within GFF5451
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 78,544 + -0.2 78,544 + -0.1 78,544 + -0.1 78,544 + -1.6 78,544 + +0.7 78,544 + -0.1 78,545 - +0.4 78,545 - -0.5 78,545 - +0.0 78,545 - +0.4 78,545 - -0.7 78,545 - +0.9 78,545 - +0.8 78,545 - -1.8 78,545 - +0.2 78,545 - +0.3 79,116 - -0.0 79,138 + -0.1 79,138 + +0.9 79,138 + -0.2 79,138 + -0.7 79,138 + +0.5 79,139 - +1.9 79,139 - -0.4 79,139 - -0.5 79,139 - -0.1 79,139 - +1.9 79,139 - +1.1 79,139 - -0.5 79,139 - +0.7 79,142 + -0.4 79,142 + +0.2 79,142 + +1.1 79,142 + -0.1 79,142 + -1.0 79,142 + +0.1 79,142 + +0.3 79,142 + +0.1 79,142 + -0.3 79,142 + +0.2 79,142 + -0.2 79,142 + +0.1 79,142 + +0.2 79,142 + -0.1 79,142 + +0.1 79,142 + +0.5 79,142 + -0.3 79,143 - +0.6 79,143 - +0.5 79,143 - -0.2 79,143 - -0.3 79,143 - -0.7 79,143 - -0.1 79,143 - -0.2 79,143 - -0.3 79,143 - -0.0 79,173 + +0.2 79,174 - -1.1 79,174 - -1.4 79,397 + GFF5449 0.23 +0.8 79,397 + GFF5449 0.23 -0.2 79,397 + GFF5449 0.23 -0.2 79,398 - GFF5449 0.23 +1.2 79,398 - GFF5449 0.23 +0.7 79,398 - GFF5449 0.23 -0.2 79,398 - GFF5449 0.23 +0.4 79,398 - GFF5449 0.23 -0.5 79,398 - GFF5449 0.23 +0.9 79,398 - GFF5449 0.23 +0.2 79,398 - GFF5449 0.23 -0.1 79,700 + GFF5449 0.69 -0.1 79,700 + GFF5449 0.69 -0.9 79,700 + GFF5449 0.69 +0.6 79,700 + GFF5449 0.69 +0.4 79,700 + GFF5449 0.69 +0.1 79,700 + GFF5449 0.69 +1.1 79,700 + GFF5449 0.69 -0.5 79,700 + GFF5449 0.69 -0.4 79,701 - GFF5449 0.69 -0.6 79,743 + GFF5449 0.75 +0.1 79,743 + GFF5449 0.75 +0.4 79,744 - GFF5449 0.75 -1.6 79,744 - GFF5449 0.75 +0.1 79,744 - GFF5449 0.75 -0.4 79,821 + GFF5449 0.87 -0.1 79,822 - GFF5449 0.87 +0.5 79,892 - -0.2 79,892 - -0.1 79,892 - -0.2 79,945 + -0.1 79,945 + -0.2 79,945 + -0.3 79,945 + -1.0 79,945 + +0.8 79,945 + -0.4 79,945 + +0.4 79,945 + -1.8 79,945 + +0.0 79,945 + -0.1 79,945 + -0.1 79,945 + -0.1 79,945 + -0.2 79,945 + -0.5 79,946 - -2.4 79,946 - -0.2 79,946 - -0.6 79,946 - +0.6 79,946 - +0.1 79,946 - -0.4 79,946 - +1.4 79,946 - -0.5 79,946 - -0.2 79,946 - +0.3 79,946 - +0.6 79,946 - -0.0 79,946 - +1.5 79,968 + -0.6 79,968 + -3.3 79,969 - -1.4 79,993 + -0.9 80,045 - -0.6 80,092 + -1.5 80,092 + -0.0 80,092 + -1.6 80,092 + -0.3 80,093 - +0.9 80,093 - +0.3 80,093 - +0.1 80,093 - -0.8 80,093 - -0.1 80,281 + -0.9 80,281 + -0.5 80,281 + +0.4 80,281 + -0.8 80,281 + -1.0 80,281 + -2.1 80,281 + -1.4 80,281 + -0.4 80,281 + +0.5 80,281 + +0.4 80,282 - -0.9 80,282 - -2.2 80,282 - -0.3 80,282 - +0.9 80,282 - -0.1 80,282 - -1.4 80,595 + GFF5451 0.29 -0.4 80,643 + GFF5451 0.33 -0.1 80,643 + GFF5451 0.33 -0.5 80,643 + GFF5451 0.33 +1.6 80,643 + GFF5451 0.33 -0.8 80,643 + GFF5451 0.33 -2.0 80,643 + GFF5451 0.33 +0.4 80,644 - GFF5451 0.33 -1.9 80,644 - GFF5451 0.33 -1.3 80,644 - GFF5451 0.33 +0.2 80,644 - GFF5451 0.33 -0.5 80,644 - GFF5451 0.33 -0.1 80,644 - GFF5451 0.33 -0.1 80,644 - GFF5451 0.33 +0.4 80,646 + GFF5451 0.33 -0.4 80,646 + GFF5451 0.33 +0.4 80,646 + GFF5451 0.33 +0.6 80,646 + GFF5451 0.33 +0.4 80,646 + GFF5451 0.33 +1.1 80,646 + GFF5451 0.33 -2.2 80,646 + GFF5451 0.33 +0.7 80,647 - GFF5451 0.33 +1.9 80,647 - GFF5451 0.33 -0.3 80,647 - GFF5451 0.33 +0.1 80,647 - GFF5451 0.33 -1.2 80,647 - GFF5451 0.33 +0.9 80,647 - GFF5451 0.33 -1.0 80,647 - GFF5451 0.33 +0.9 80,647 - GFF5451 0.33 -0.6 80,658 + GFF5451 0.34 -0.7 80,658 + GFF5451 0.34 -0.5 80,658 + GFF5451 0.34 -0.4 80,658 + GFF5451 0.34 -0.8 80,658 + GFF5451 0.34 +0.9 80,658 + GFF5451 0.34 +0.9 80,658 + GFF5451 0.34 +0.4 80,658 + GFF5451 0.34 +1.1 80,659 - GFF5451 0.34 +0.5 80,659 - GFF5451 0.34 -0.1 80,659 - GFF5451 0.34 +0.9 80,659 - GFF5451 0.34 +0.9 80,659 - GFF5451 0.34 +0.5 80,659 - GFF5451 0.34 -0.2 80,659 - GFF5451 0.34 -0.6 80,703 + GFF5451 0.38 +0.7 80,703 + GFF5451 0.38 +0.6 80,703 + GFF5451 0.38 -0.4 80,704 - GFF5451 0.38 -2.8 80,704 - GFF5451 0.38 +0.4 80,704 - GFF5451 0.38 -0.4 80,704 - GFF5451 0.38 -0.7 80,704 - GFF5451 0.38 -1.1 80,704 - GFF5451 0.38 -0.6 80,742 + GFF5451 0.42 -0.8 80,742 + GFF5451 0.42 -0.4 80,742 + GFF5451 0.42 +1.8 80,743 - GFF5451 0.42 -1.4 80,887 - GFF5451 0.55 -1.1
Or see this region's nucleotide sequence