Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5093 and GFF5094 are separated by 2 nucleotides GFF5094 and GFF5095 are separated by 3 nucleotides GFF5095 and GFF5096 are separated by 85 nucleotides GFF5096 and GFF5097 are separated by 13 nucleotides
GFF5093 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 195,580 to 197,043
GFF5093
GFF5094 - BUG/TctC family periplasmic protein, at 197,046 to 198,014
GFF5094
GFF5095 - hypothetical protein, at 198,018 to 198,353
GFF5095
GFF5096 - similar to protocatechuate 4,5-dioxygenase beta chain, at 198,439 to 199,266
GFF5096
GFF5097 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 199,280 to 199,687
GFF5097
Position (kb) 198
199 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 197.304 kb on + strand, within GFF5094 at 197.304 kb on + strand, within GFF5094 at 197.368 kb on - strand, within GFF5094 at 197.593 kb on - strand, within GFF5094 at 197.593 kb on - strand, within GFF5094 at 197.770 kb on + strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.970 kb on + strand at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.252 kb on + strand, within GFF5095 at 198.285 kb on + strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.349 kb on + strand at 198.450 kb on - strand at 198.640 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.121 kb on - strand, within GFF5096 at 199.301 kb on + strand at 199.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 197,304 + GFF5094 0.27 -0.7 197,304 + GFF5094 0.27 -0.4 197,368 - GFF5094 0.33 -0.7 197,593 - GFF5094 0.56 -0.3 197,593 - GFF5094 0.56 -0.4 197,770 + GFF5094 0.75 +0.5 197,771 - GFF5094 0.75 -0.1 197,771 - GFF5094 0.75 +0.3 197,970 + -0.1 198,183 + GFF5095 0.49 +0.1 198,183 + GFF5095 0.49 -0.9 198,183 + GFF5095 0.49 +0.4 198,183 + GFF5095 0.49 +0.9 198,184 - GFF5095 0.49 +0.9 198,184 - GFF5095 0.49 +1.4 198,252 + GFF5095 0.70 -0.0 198,285 + GFF5095 0.79 +0.6 198,286 - GFF5095 0.80 -1.1 198,286 - GFF5095 0.80 +0.4 198,309 + GFF5095 0.87 +3.2 198,309 + GFF5095 0.87 -1.3 198,309 + GFF5095 0.87 -0.1 198,309 + GFF5095 0.87 -0.8 198,309 + GFF5095 0.87 -0.8 198,309 + GFF5095 0.87 -0.3 198,309 + GFF5095 0.87 +1.0 198,309 + GFF5095 0.87 +0.6 198,310 - GFF5095 0.87 -1.1 198,310 - GFF5095 0.87 +0.9 198,310 - GFF5095 0.87 -2.6 198,349 + -0.6 198,450 - -0.5 198,640 + GFF5096 0.24 -1.4 198,664 + GFF5096 0.27 -1.0 198,664 + GFF5096 0.27 -0.6 198,665 - GFF5096 0.27 -3.1 198,665 - GFF5096 0.27 -0.0 198,758 + GFF5096 0.39 +0.3 198,758 + GFF5096 0.39 -2.2 199,120 + GFF5096 0.82 +0.3 199,120 + GFF5096 0.82 -0.1 199,121 - GFF5096 0.82 -0.8 199,301 + -1.2 199,302 - -0.4
Or see this region's nucleotide sequence