Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5083 and GFF5084 are separated by 7 nucleotides GFF5084 and GFF5085 are separated by 184 nucleotides GFF5085 and GFF5086 are separated by 62 nucleotides
GFF5083 - Transcriptional regulator, LysR family, at 186,886 to 187,833
GFF5083
GFF5084 - FIG00933367: hypothetical protein, at 187,841 to 188,278
GFF5084
GFF5085 - hypothetical protein, at 188,463 to 188,918
GFF5085
GFF5086 - Glycine betaine transporter OpuD, at 188,981 to 190,495
GFF5086
Position (kb) 188
189 Strain fitness (log2 ratio) -2
-1
0
1 at 187.830 kb on + strand at 187.831 kb on + strand at 187.831 kb on + strand at 187.831 kb on + strand at 187.831 kb on + strand at 187.831 kb on + strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.941 kb on + strand, within GFF5084 at 187.942 kb on - strand, within GFF5084 at 187.942 kb on - strand, within GFF5084 at 187.942 kb on - strand, within GFF5084 at 187.942 kb on - strand, within GFF5084 at 187.968 kb on + strand, within GFF5084 at 187.968 kb on + strand, within GFF5084 at 187.969 kb on - strand, within GFF5084 at 187.969 kb on - strand, within GFF5084 at 188.091 kb on + strand, within GFF5084 at 188.091 kb on + strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.343 kb on + strand at 188.378 kb on + strand at 188.378 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.463 kb on - strand at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.542 kb on - strand, within GFF5085 at 188.542 kb on - strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.640 kb on + strand, within GFF5085 at 188.641 kb on - strand, within GFF5085 at 188.667 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.836 kb on - strand, within GFF5085 at 188.925 kb on + strand at 188.926 kb on - strand at 188.926 kb on - strand at 188.926 kb on - strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.132 kb on - strand at 189.132 kb on - strand at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.577 kb on - strand, within GFF5086 at 189.577 kb on - strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 187,830 + -0.9 187,831 + -0.3 187,831 + -0.8 187,831 + -1.0 187,831 + -0.2 187,831 + +0.9 187,832 - -0.0 187,832 - -0.1 187,832 - -0.9 187,832 - +0.4 187,832 - -0.3 187,832 - +0.3 187,832 - +0.9 187,832 - -0.3 187,832 - -1.2 187,941 + GFF5084 0.23 +0.1 187,942 - GFF5084 0.23 -1.0 187,942 - GFF5084 0.23 +1.5 187,942 - GFF5084 0.23 -0.7 187,942 - GFF5084 0.23 +0.0 187,968 + GFF5084 0.29 -0.4 187,968 + GFF5084 0.29 +0.6 187,969 - GFF5084 0.29 +0.0 187,969 - GFF5084 0.29 -1.0 188,091 + GFF5084 0.57 -2.4 188,091 + GFF5084 0.57 +0.4 188,092 - GFF5084 0.57 -0.3 188,092 - GFF5084 0.57 +0.0 188,092 - GFF5084 0.57 -0.2 188,343 + -0.9 188,378 + -1.9 188,378 + -0.7 188,462 + -0.1 188,462 + +0.4 188,462 + -0.2 188,462 + -1.3 188,462 + -1.9 188,463 - -0.3 188,541 + GFF5085 0.17 -1.3 188,541 + GFF5085 0.17 -0.4 188,541 + GFF5085 0.17 -1.2 188,541 + GFF5085 0.17 -0.8 188,541 + GFF5085 0.17 -1.1 188,542 - GFF5085 0.17 -0.3 188,542 - GFF5085 0.17 +1.1 188,592 + GFF5085 0.28 +0.5 188,592 + GFF5085 0.28 -1.2 188,592 + GFF5085 0.28 +0.3 188,592 + GFF5085 0.28 -0.9 188,592 + GFF5085 0.28 +1.1 188,592 + GFF5085 0.28 -1.9 188,592 + GFF5085 0.28 -1.9 188,592 + GFF5085 0.28 -1.6 188,592 + GFF5085 0.28 -0.8 188,592 + GFF5085 0.28 -1.5 188,592 + GFF5085 0.28 -1.3 188,593 - GFF5085 0.29 -0.1 188,593 - GFF5085 0.29 -0.4 188,593 - GFF5085 0.29 -0.6 188,593 - GFF5085 0.29 -0.9 188,593 - GFF5085 0.29 -0.3 188,593 - GFF5085 0.29 +0.5 188,640 + GFF5085 0.39 -1.4 188,641 - GFF5085 0.39 -1.4 188,667 + GFF5085 0.45 -1.0 188,835 + GFF5085 0.82 +0.5 188,835 + GFF5085 0.82 -1.6 188,835 + GFF5085 0.82 -1.3 188,836 - GFF5085 0.82 -1.6 188,925 + +0.1 188,926 - -0.4 188,926 - +0.4 188,926 - -0.1 189,131 + -0.4 189,131 + -0.2 189,131 + +1.1 189,131 + -1.0 189,131 + +1.2 189,132 - -0.0 189,132 - -0.4 189,368 + GFF5086 0.26 +0.8 189,368 + GFF5086 0.26 +0.2 189,368 + GFF5086 0.26 +0.9 189,368 + GFF5086 0.26 -1.6 189,368 + GFF5086 0.26 -0.5 189,369 - GFF5086 0.26 -0.2 189,369 - GFF5086 0.26 -0.1 189,369 - GFF5086 0.26 +0.0 189,369 - GFF5086 0.26 +0.5 189,369 - GFF5086 0.26 -0.1 189,577 - GFF5086 0.39 -0.2 189,577 - GFF5086 0.39 -0.7 189,657 + GFF5086 0.45 -0.1 189,657 + GFF5086 0.45 -0.7 189,657 + GFF5086 0.45 -0.3 189,658 - GFF5086 0.45 -0.5 189,658 - GFF5086 0.45 +1.0 189,658 - GFF5086 0.45 +0.1 189,658 - GFF5086 0.45 -2.4
Or see this region's nucleotide sequence