Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5052 and GFF5053 overlap by 4 nucleotides GFF5053 and GFF5054 are separated by 59 nucleotides GFF5054 and GFF5055 overlap by 4 nucleotides GFF5055 and GFF5056 are separated by 22 nucleotides
GFF5052 - FAD-dependent oxidoreductase SPO0682, at 152,900 to 154,540
GFF5052
GFF5053 - Uncharacterized protein SPO0681, at 154,537 to 154,749
GFF5053
GFF5054 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 154,809 to 156,119
GFF5054
GFF5055 - BUG/TctC family periplasmic protein, at 156,116 to 157,090
GFF5055
GFF5056 - Fumarylacetoacetase (EC 3.7.1.2), at 157,113 to 158,378
GFF5056
Position (kb)
154
155
156
157 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 153.912 kb on + strand, within GFF5052 at 153.912 kb on + strand, within GFF5052 at 153.913 kb on - strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.959 kb on + strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 153.960 kb on - strand, within GFF5052 at 154.419 kb on - strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.442 kb on + strand at 154.443 kb on - strand at 154.443 kb on - strand at 154.606 kb on + strand, within GFF5053 at 154.606 kb on + strand, within GFF5053 at 154.607 kb on - strand, within GFF5053 at 154.802 kb on - strand at 154.842 kb on + strand at 154.843 kb on - strand at 154.843 kb on - strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.866 kb on + strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.867 kb on - strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.938 kb on + strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 154.939 kb on - strand at 155.004 kb on + strand, within GFF5054 at 155.037 kb on + strand, within GFF5054 at 155.038 kb on - strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.046 kb on + strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.047 kb on - strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.416 kb on - strand, within GFF5054 at 155.430 kb on + strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.541 kb on + strand, within GFF5054 at 155.542 kb on - strand, within GFF5054 at 155.644 kb on - strand, within GFF5054 at 155.841 kb on + strand, within GFF5054 at 155.862 kb on + strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 156.075 kb on + strand at 156.075 kb on + strand at 156.076 kb on - strand at 156.198 kb on - strand at 156.198 kb on - strand at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.582 kb on - strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.755 kb on - strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 157.040 kb on + strand at 157.040 kb on + strand at 157.040 kb on + strand at 157.041 kb on - strand at 157.041 kb on - strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.109 kb on - strand at 157.109 kb on - strand at 157.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 153,912 + GFF5052 0.62 +0.1 153,912 + GFF5052 0.62 +0.1 153,913 - GFF5052 0.62 +1.3 153,959 + GFF5052 0.65 +0.8 153,959 + GFF5052 0.65 -0.4 153,959 + GFF5052 0.65 -1.7 153,959 + GFF5052 0.65 +1.9 153,959 + GFF5052 0.65 -0.9 153,959 + GFF5052 0.65 -0.1 153,959 + GFF5052 0.65 -0.1 153,959 + GFF5052 0.65 +0.4 153,959 + GFF5052 0.65 -1.9 153,959 + GFF5052 0.65 +0.7 153,959 + GFF5052 0.65 -0.3 153,959 + GFF5052 0.65 -0.1 153,959 + GFF5052 0.65 +0.9 153,959 + GFF5052 0.65 -0.0 153,959 + GFF5052 0.65 +0.3 153,959 + GFF5052 0.65 -0.2 153,959 + GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 +0.0 153,960 - GFF5052 0.65 -0.8 153,960 - GFF5052 0.65 +1.3 153,960 - GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 +0.0 153,960 - GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 -0.2 153,960 - GFF5052 0.65 -0.3 153,960 - GFF5052 0.65 -1.2 153,960 - GFF5052 0.65 -1.3 153,960 - GFF5052 0.65 -1.1 153,960 - GFF5052 0.65 -0.1 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -0.5 153,960 - GFF5052 0.65 +1.5 153,960 - GFF5052 0.65 -0.7 153,960 - GFF5052 0.65 -0.3 153,960 - GFF5052 0.65 -1.4 154,419 - -0.9 154,442 + -0.6 154,442 + -1.2 154,442 + -0.7 154,443 - -0.1 154,443 - -0.9 154,606 + GFF5053 0.32 -0.3 154,606 + GFF5053 0.32 +0.3 154,607 - GFF5053 0.33 -0.0 154,802 - +1.0 154,842 + -0.2 154,843 - -0.3 154,843 - -0.8 154,866 + +0.1 154,866 + -0.6 154,866 + +0.9 154,866 + -0.6 154,866 + -0.9 154,867 - +2.9 154,867 - -1.0 154,867 - +0.1 154,938 + -0.6 154,938 + -0.5 154,938 + -2.4 154,939 - -0.3 154,939 - +0.6 154,939 - -0.9 154,939 - -0.5 154,939 - -0.3 155,004 + GFF5054 0.15 -1.9 155,037 + GFF5054 0.17 -0.2 155,038 - GFF5054 0.17 +1.1 155,046 + GFF5054 0.18 -0.5 155,046 + GFF5054 0.18 -0.6 155,046 + GFF5054 0.18 -0.7 155,046 + GFF5054 0.18 -0.2 155,047 - GFF5054 0.18 +0.2 155,047 - GFF5054 0.18 -0.6 155,047 - GFF5054 0.18 -0.0 155,047 - GFF5054 0.18 -0.3 155,047 - GFF5054 0.18 +0.1 155,223 + GFF5054 0.32 +0.6 155,223 + GFF5054 0.32 -0.7 155,223 + GFF5054 0.32 -0.3 155,223 + GFF5054 0.32 -1.6 155,223 + GFF5054 0.32 +0.5 155,223 + GFF5054 0.32 -0.4 155,224 - GFF5054 0.32 +0.3 155,224 - GFF5054 0.32 -1.0 155,224 - GFF5054 0.32 +0.4 155,224 - GFF5054 0.32 -0.6 155,224 - GFF5054 0.32 -0.1 155,224 - GFF5054 0.32 -0.2 155,224 - GFF5054 0.32 -0.0 155,415 + GFF5054 0.46 -0.8 155,415 + GFF5054 0.46 -0.4 155,416 - GFF5054 0.46 -1.2 155,430 + GFF5054 0.47 -0.1 155,431 - GFF5054 0.47 -0.2 155,431 - GFF5054 0.47 -2.4 155,431 - GFF5054 0.47 -0.4 155,431 - GFF5054 0.47 +1.1 155,541 + GFF5054 0.56 -0.8 155,542 - GFF5054 0.56 +0.2 155,644 - GFF5054 0.64 -0.3 155,841 + GFF5054 0.79 -0.2 155,862 + GFF5054 0.80 -0.5 155,863 - GFF5054 0.80 -1.0 155,863 - GFF5054 0.80 -0.4 155,863 - GFF5054 0.80 +0.7 155,863 - GFF5054 0.80 +1.4 156,075 + -1.2 156,075 + +0.1 156,076 - -1.0 156,198 - -0.6 156,198 - +1.4 156,230 + GFF5055 0.12 -2.3 156,230 + GFF5055 0.12 -0.4 156,230 + GFF5055 0.12 -0.2 156,230 + GFF5055 0.12 +0.1 156,230 + GFF5055 0.12 -1.2 156,230 + GFF5055 0.12 -0.2 156,231 - GFF5055 0.12 +0.1 156,231 - GFF5055 0.12 -0.4 156,231 - GFF5055 0.12 +0.1 156,231 - GFF5055 0.12 -0.6 156,380 + GFF5055 0.27 -0.2 156,380 + GFF5055 0.27 +0.0 156,380 + GFF5055 0.27 +0.8 156,381 - GFF5055 0.27 +0.9 156,381 - GFF5055 0.27 +1.3 156,381 - GFF5055 0.27 +1.3 156,381 - GFF5055 0.27 +1.3 156,381 - GFF5055 0.27 -2.3 156,381 - GFF5055 0.27 -0.3 156,431 + GFF5055 0.32 +1.7 156,431 + GFF5055 0.32 +0.2 156,431 + GFF5055 0.32 -1.6 156,431 + GFF5055 0.32 +2.3 156,431 + GFF5055 0.32 -0.2 156,431 + GFF5055 0.32 +1.3 156,431 + GFF5055 0.32 -1.4 156,431 + GFF5055 0.32 -0.1 156,432 - GFF5055 0.32 -0.2 156,432 - GFF5055 0.32 +0.9 156,432 - GFF5055 0.32 +0.5 156,432 - GFF5055 0.32 +0.1 156,432 - GFF5055 0.32 +0.8 156,432 - GFF5055 0.32 -0.4 156,432 - GFF5055 0.32 -0.5 156,432 - GFF5055 0.32 +0.3 156,581 + GFF5055 0.48 -0.2 156,581 + GFF5055 0.48 +0.7 156,582 - GFF5055 0.48 -0.8 156,668 + GFF5055 0.57 -0.9 156,668 + GFF5055 0.57 +0.5 156,668 + GFF5055 0.57 -0.4 156,668 + GFF5055 0.57 -1.1 156,668 + GFF5055 0.57 -0.4 156,668 + GFF5055 0.57 -0.5 156,668 + GFF5055 0.57 +1.9 156,668 + GFF5055 0.57 -0.4 156,669 - GFF5055 0.57 -0.1 156,669 - GFF5055 0.57 -0.2 156,669 - GFF5055 0.57 -0.1 156,669 - GFF5055 0.57 +0.7 156,669 - GFF5055 0.57 -1.8 156,755 - GFF5055 0.66 -0.7 156,881 + GFF5055 0.78 -0.7 156,881 + GFF5055 0.78 -0.7 156,881 + GFF5055 0.78 +0.7 156,882 - GFF5055 0.79 -0.1 156,882 - GFF5055 0.79 +0.5 156,882 - GFF5055 0.79 -0.1 156,882 - GFF5055 0.79 -0.7 157,040 + -0.7 157,040 + -0.2 157,040 + -1.1 157,041 - -0.3 157,041 - -0.6 157,108 + +0.5 157,108 + +1.3 157,108 + -1.1 157,108 + -1.5 157,108 + -0.5 157,108 + -1.3 157,108 + +0.2 157,109 - +1.4 157,109 - +0.1 157,109 - +0.3
Or see this region's nucleotide sequence