Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4970 and GFF4971 are separated by 24 nucleotides GFF4971 and GFF4972 overlap by 8 nucleotides GFF4972 and GFF4973 overlap by 4 nucleotides GFF4973 and GFF4974 overlap by 4 nucleotides
GFF4970 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,987 to 71,736
GFF4970
GFF4971 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 71,761 to 72,480
GFF4971
GFF4972 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 72,473 to 73,237
GFF4972
GFF4973 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 73,234 to 74,217
GFF4973
GFF4974 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 74,214 to 75,116
GFF4974
Position (kb) 72
73
74 Strain fitness (log2 ratio) -1
0
1
2 at 71.853 kb on - strand, within GFF4971 at 71.853 kb on - strand, within GFF4971 at 71.858 kb on + strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.438 kb on - strand at 72.501 kb on + strand at 72.501 kb on + strand at 72.502 kb on - strand at 72.503 kb on - strand at 73.071 kb on + strand, within GFF4972 at 73.071 kb on + strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.072 kb on - strand, within GFF4972 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.352 kb on + strand, within GFF4973 at 73.353 kb on - strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.532 kb on + strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.533 kb on - strand, within GFF4973 at 73.727 kb on + strand, within GFF4973 at 73.727 kb on + strand, within GFF4973 at 73.754 kb on + strand, within GFF4973 at 73.754 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.871 kb on + strand, within GFF4973 at 73.872 kb on - strand, within GFF4973 at 73.872 kb on - strand, within GFF4973 at 73.997 kb on + strand, within GFF4973 at 73.997 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.111 kb on + strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.112 kb on - strand, within GFF4973 at 74.120 kb on + strand at 74.218 kb on - strand at 74.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 71,853 - GFF4971 0.13 +1.1 71,853 - GFF4971 0.13 -0.8 71,858 + GFF4971 0.13 +0.1 71,859 - GFF4971 0.14 -0.1 71,859 - GFF4971 0.14 +0.0 72,117 - GFF4971 0.49 -0.0 72,117 - GFF4971 0.49 +0.3 72,165 - GFF4971 0.56 -0.5 72,165 - GFF4971 0.56 -1.1 72,165 - GFF4971 0.56 -0.9 72,438 - +1.1 72,501 + -0.4 72,501 + -0.4 72,502 - +1.2 72,503 - -1.1 73,071 + GFF4972 0.78 +0.3 73,071 + GFF4972 0.78 +0.9 73,072 - GFF4972 0.78 -0.3 73,072 - GFF4972 0.78 +1.9 73,352 + GFF4973 0.12 -0.1 73,352 + GFF4973 0.12 -0.2 73,352 + GFF4973 0.12 -0.6 73,353 - GFF4973 0.12 -0.6 73,532 + GFF4973 0.30 +1.0 73,532 + GFF4973 0.30 -1.1 73,532 + GFF4973 0.30 +1.9 73,533 - GFF4973 0.30 +0.5 73,533 - GFF4973 0.30 -0.4 73,533 - GFF4973 0.30 -1.0 73,533 - GFF4973 0.30 -0.3 73,533 - GFF4973 0.30 -0.4 73,727 + GFF4973 0.50 -0.5 73,727 + GFF4973 0.50 -0.9 73,754 + GFF4973 0.53 -1.0 73,754 + GFF4973 0.53 -1.5 73,871 + GFF4973 0.65 -1.1 73,871 + GFF4973 0.65 -0.1 73,871 + GFF4973 0.65 +0.7 73,872 - GFF4973 0.65 +1.9 73,872 - GFF4973 0.65 -1.7 73,997 + GFF4973 0.78 -0.3 73,997 + GFF4973 0.78 +0.1 74,111 + GFF4973 0.89 -0.4 74,111 + GFF4973 0.89 +0.4 74,111 + GFF4973 0.89 -0.6 74,111 + GFF4973 0.89 -0.6 74,112 - GFF4973 0.89 +0.9 74,112 - GFF4973 0.89 -0.1 74,112 - GFF4973 0.89 +0.9 74,120 + -0.7 74,218 - -0.1 74,218 - -0.0
Or see this region's nucleotide sequence