Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4953 and GFF4954 are separated by 28 nucleotides GFF4954 and GFF4955 overlap by 4 nucleotides GFF4955 and GFF4956 are separated by 228 nucleotides GFF4956 and GFF4957 are separated by 164 nucleotides
GFF4953 - Beta-galactosidase (EC 3.2.1.23), at 49,460 to 54,172
GFF4953
GFF4954 - no description, at 54,201 to 54,914
GFF4954
GFF4955 - hypothetical protein, at 54,911 to 56,917
GFF4955
GFF4956 - ATP synthase F0 sector subunit c (EC 3.6.3.14), at 57,146 to 57,385
GFF4956
GFF4957 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 57,550 to 58,755
GFF4957
Position (kb)
54
55
56
57 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 53.919 kb on - strand at 53.922 kb on + strand at 53.923 kb on - strand at 53.923 kb on - strand at 53.923 kb on - strand at 54.617 kb on - strand, within GFF4954 at 54.617 kb on - strand, within GFF4954 at 54.797 kb on - strand, within GFF4954 at 54.896 kb on - strand at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.824 kb on + strand, within GFF4955 at 55.825 kb on - strand, within GFF4955 at 55.825 kb on - strand, within GFF4955 at 56.394 kb on + strand, within GFF4955 at 56.395 kb on - strand, within GFF4955 at 56.395 kb on - strand, within GFF4955 at 56.403 kb on + strand, within GFF4955 at 56.484 kb on + strand, within GFF4955 at 56.485 kb on - strand, within GFF4955 at 56.487 kb on + strand, within GFF4955 at 56.487 kb on + strand, within GFF4955 at 56.487 kb on + strand, within GFF4955 at 56.487 kb on + strand, within GFF4955 at 56.488 kb on - strand, within GFF4955 at 56.488 kb on - strand, within GFF4955 at 56.488 kb on - strand, within GFF4955 at 56.488 kb on - strand, within GFF4955 at 56.488 kb on - strand, within GFF4955 at 56.610 kb on + strand, within GFF4955 at 56.610 kb on + strand, within GFF4955 at 56.610 kb on + strand, within GFF4955 at 57.099 kb on + strand at 57.434 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 53,919 - +0.1 53,922 + -0.6 53,923 - +0.2 53,923 - -1.5 53,923 - -0.1 54,617 - GFF4954 0.58 +2.1 54,617 - GFF4954 0.58 -0.9 54,797 - GFF4954 0.83 +0.7 54,896 - -1.6 55,824 + GFF4955 0.45 +0.4 55,824 + GFF4955 0.45 +0.3 55,824 + GFF4955 0.45 +2.2 55,824 + GFF4955 0.45 -1.9 55,824 + GFF4955 0.45 -0.4 55,824 + GFF4955 0.45 -0.9 55,824 + GFF4955 0.45 -0.1 55,824 + GFF4955 0.45 +0.3 55,825 - GFF4955 0.46 -1.2 55,825 - GFF4955 0.46 -0.2 56,394 + GFF4955 0.74 -1.4 56,395 - GFF4955 0.74 +0.1 56,395 - GFF4955 0.74 -0.1 56,403 + GFF4955 0.74 -0.0 56,484 + GFF4955 0.78 +0.9 56,485 - GFF4955 0.78 +0.9 56,487 + GFF4955 0.79 +0.9 56,487 + GFF4955 0.79 +0.9 56,487 + GFF4955 0.79 -1.3 56,487 + GFF4955 0.79 +1.9 56,488 - GFF4955 0.79 +0.4 56,488 - GFF4955 0.79 -0.1 56,488 - GFF4955 0.79 -0.2 56,488 - GFF4955 0.79 +0.8 56,488 - GFF4955 0.79 -1.9 56,610 + GFF4955 0.85 -1.1 56,610 + GFF4955 0.85 +0.1 56,610 + GFF4955 0.85 -0.7 57,099 + -0.4 57,434 + +0.2
Or see this region's nucleotide sequence