Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4418 and GFF4419 are separated by 12 nucleotides GFF4419 and GFF4420 overlap by 4 nucleotides GFF4420 and GFF4421 are separated by 12 nucleotides GFF4421 and GFF4422 overlap by 4 nucleotides
GFF4418 - Conjugative transfer protein TrbJ, at 55,153 to 55,908
GFF4418
GFF4419 - Conjugative transfer protein TrbK, at 55,921 to 56,205
GFF4419
GFF4420 - Conjugative transfer protein TrbL, at 56,202 to 57,581
GFF4420
GFF4421 - Conjugative transfer protein TrbF, at 57,594 to 58,298
GFF4421
GFF4422 - Conjugative transfer protein TrbG, at 58,295 to 59,293
GFF4422
Position (kb) 56
57
58 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 55.468 kb on + strand, within GFF4418 at 55.468 kb on + strand, within GFF4418 at 55.468 kb on + strand, within GFF4418 at 55.468 kb on + strand, within GFF4418 at 55.468 kb on + strand, within GFF4418 at 55.508 kb on - strand, within GFF4418 at 55.519 kb on + strand, within GFF4418 at 55.519 kb on + strand, within GFF4418 at 55.519 kb on + strand, within GFF4418 at 55.519 kb on + strand, within GFF4418 at 55.519 kb on + strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.520 kb on - strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.549 kb on + strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.550 kb on - strand, within GFF4418 at 55.733 kb on + strand, within GFF4418 at 55.733 kb on + strand, within GFF4418 at 55.734 kb on - strand, within GFF4418 at 55.766 kb on + strand, within GFF4418 at 55.767 kb on - strand, within GFF4418 at 55.873 kb on + strand at 55.873 kb on + strand at 55.873 kb on + strand at 55.873 kb on + strand at 55.874 kb on - strand at 55.874 kb on - strand at 55.897 kb on + strand at 55.897 kb on + strand at 55.898 kb on - strand at 56.141 kb on - strand, within GFF4419 at 56.246 kb on + strand at 56.246 kb on + strand at 56.246 kb on + strand at 56.246 kb on + strand at 56.246 kb on + strand at 56.246 kb on + strand at 56.246 kb on + strand at 56.247 kb on - strand at 56.247 kb on - strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.250 kb on + strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.251 kb on - strand at 56.340 kb on + strand, within GFF4420 at 56.340 kb on + strand, within GFF4420 at 56.340 kb on + strand, within GFF4420 at 56.340 kb on + strand, within GFF4420 at 56.340 kb on + strand, within GFF4420 at 56.340 kb on + strand, within GFF4420 at 56.341 kb on - strand, within GFF4420 at 56.341 kb on - strand, within GFF4420 at 56.341 kb on - strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.409 kb on + strand, within GFF4420 at 56.410 kb on - strand, within GFF4420 at 56.410 kb on - strand, within GFF4420 at 56.410 kb on - strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.427 kb on + strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.428 kb on - strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.560 kb on + strand, within GFF4420 at 56.561 kb on - strand, within GFF4420 at 56.561 kb on - strand, within GFF4420 at 56.860 kb on + strand, within GFF4420 at 56.860 kb on + strand, within GFF4420 at 56.860 kb on + strand, within GFF4420 at 56.861 kb on - strand, within GFF4420 at 56.861 kb on - strand, within GFF4420 at 57.579 kb on + strand at 57.579 kb on + strand at 57.579 kb on + strand at 57.579 kb on + strand at 57.580 kb on - strand at 57.580 kb on - strand at 57.580 kb on - strand at 57.580 kb on - strand at 57.580 kb on - strand at 57.580 kb on - strand at 57.622 kb on - strand at 57.651 kb on + strand at 57.651 kb on + strand at 57.652 kb on - strand at 57.652 kb on - strand at 57.652 kb on - strand at 57.670 kb on + strand, within GFF4421 at 57.670 kb on + strand, within GFF4421 at 57.670 kb on + strand, within GFF4421 at 57.670 kb on + strand, within GFF4421 at 57.670 kb on + strand, within GFF4421 at 57.670 kb on + strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.671 kb on - strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.804 kb on + strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.805 kb on - strand, within GFF4421 at 57.864 kb on + strand, within GFF4421 at 57.972 kb on + strand, within GFF4421 at 57.973 kb on - strand, within GFF4421 at 57.978 kb on + strand, within GFF4421 at 57.978 kb on + strand, within GFF4421 at 57.979 kb on - strand, within GFF4421 at 57.979 kb on - strand, within GFF4421 at 58.014 kb on + strand, within GFF4421 at 58.015 kb on - strand, within GFF4421 at 58.015 kb on - strand, within GFF4421 at 58.131 kb on + strand, within GFF4421 at 58.131 kb on + strand, within GFF4421 at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.239 kb on + strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.240 kb on - strand at 58.473 kb on + strand, within GFF4422 at 58.473 kb on + strand, within GFF4422 at 58.473 kb on + strand, within GFF4422 at 58.473 kb on + strand, within GFF4422 at 58.473 kb on + strand, within GFF4422 at 58.473 kb on + strand, within GFF4422 at 58.474 kb on - strand, within GFF4422 at 58.474 kb on - strand, within GFF4422 at 58.474 kb on - strand, within GFF4422 at 58.474 kb on - strand, within GFF4422 at 58.474 kb on - strand, within GFF4422 at 58.474 kb on - strand, within GFF4422
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 55,468 + GFF4418 0.42 -0.8 55,468 + GFF4418 0.42 -1.0 55,468 + GFF4418 0.42 -0.5 55,468 + GFF4418 0.42 -0.5 55,468 + GFF4418 0.42 -1.0 55,508 - GFF4418 0.47 -0.1 55,519 + GFF4418 0.48 +0.5 55,519 + GFF4418 0.48 -0.5 55,519 + GFF4418 0.48 +0.8 55,519 + GFF4418 0.48 +0.3 55,519 + GFF4418 0.48 -0.2 55,520 - GFF4418 0.49 -0.1 55,520 - GFF4418 0.49 +0.7 55,520 - GFF4418 0.49 -0.6 55,520 - GFF4418 0.49 -0.1 55,520 - GFF4418 0.49 -0.4 55,520 - GFF4418 0.49 -0.3 55,520 - GFF4418 0.49 +0.3 55,549 + GFF4418 0.52 +0.0 55,549 + GFF4418 0.52 -0.3 55,549 + GFF4418 0.52 -0.3 55,549 + GFF4418 0.52 -0.0 55,549 + GFF4418 0.52 -0.6 55,549 + GFF4418 0.52 -1.6 55,549 + GFF4418 0.52 -1.7 55,549 + GFF4418 0.52 -0.6 55,550 - GFF4418 0.53 +0.3 55,550 - GFF4418 0.53 +0.8 55,550 - GFF4418 0.53 -0.6 55,550 - GFF4418 0.53 +0.0 55,550 - GFF4418 0.53 -2.5 55,550 - GFF4418 0.53 -0.2 55,550 - GFF4418 0.53 +0.6 55,550 - GFF4418 0.53 +0.3 55,550 - GFF4418 0.53 +0.3 55,550 - GFF4418 0.53 +0.4 55,550 - GFF4418 0.53 +0.4 55,550 - GFF4418 0.53 +0.9 55,550 - GFF4418 0.53 -0.9 55,550 - GFF4418 0.53 -2.5 55,550 - GFF4418 0.53 -0.0 55,733 + GFF4418 0.77 +0.7 55,733 + GFF4418 0.77 -1.4 55,734 - GFF4418 0.77 +0.1 55,766 + GFF4418 0.81 +0.3 55,767 - GFF4418 0.81 -0.0 55,873 + -1.0 55,873 + +0.8 55,873 + -0.2 55,873 + -0.2 55,874 - -0.7 55,874 - -1.6 55,897 + -0.1 55,897 + -2.0 55,898 - -0.2 56,141 - GFF4419 0.77 -1.0 56,246 + +1.8 56,246 + -1.7 56,246 + -1.6 56,246 + +0.7 56,246 + +0.2 56,246 + +0.1 56,246 + -0.3 56,247 - -0.1 56,247 - -0.4 56,250 + -0.4 56,250 + +1.1 56,250 + -0.5 56,250 + -1.9 56,250 + +0.4 56,250 + +0.9 56,250 + -0.2 56,250 + -0.1 56,250 + -0.4 56,250 + -1.7 56,251 - +0.7 56,251 - +0.2 56,251 - -0.1 56,251 - -0.6 56,251 - +0.7 56,251 - +0.8 56,251 - -0.2 56,251 - +0.5 56,251 - -1.6 56,251 - -1.7 56,340 + GFF4420 0.10 +0.2 56,340 + GFF4420 0.10 -0.3 56,340 + GFF4420 0.10 +0.6 56,340 + GFF4420 0.10 +1.0 56,340 + GFF4420 0.10 -0.3 56,340 + GFF4420 0.10 -0.6 56,341 - GFF4420 0.10 -1.4 56,341 - GFF4420 0.10 +0.1 56,341 - GFF4420 0.10 -0.4 56,409 + GFF4420 0.15 +0.4 56,409 + GFF4420 0.15 -0.3 56,409 + GFF4420 0.15 -0.8 56,409 + GFF4420 0.15 -0.2 56,409 + GFF4420 0.15 +0.7 56,409 + GFF4420 0.15 -0.3 56,409 + GFF4420 0.15 +1.4 56,410 - GFF4420 0.15 -0.4 56,410 - GFF4420 0.15 -0.2 56,410 - GFF4420 0.15 +0.4 56,427 + GFF4420 0.16 -1.4 56,427 + GFF4420 0.16 -0.8 56,427 + GFF4420 0.16 +0.1 56,427 + GFF4420 0.16 -0.1 56,427 + GFF4420 0.16 +0.8 56,427 + GFF4420 0.16 +1.1 56,427 + GFF4420 0.16 -0.4 56,427 + GFF4420 0.16 -0.4 56,428 - GFF4420 0.16 -0.1 56,428 - GFF4420 0.16 -0.4 56,428 - GFF4420 0.16 -0.2 56,428 - GFF4420 0.16 -0.1 56,428 - GFF4420 0.16 -0.2 56,428 - GFF4420 0.16 +0.9 56,428 - GFF4420 0.16 +0.2 56,428 - GFF4420 0.16 +1.2 56,428 - GFF4420 0.16 -0.5 56,428 - GFF4420 0.16 -1.2 56,560 + GFF4420 0.26 -0.0 56,560 + GFF4420 0.26 -0.4 56,560 + GFF4420 0.26 +0.3 56,560 + GFF4420 0.26 -0.7 56,560 + GFF4420 0.26 -0.2 56,560 + GFF4420 0.26 -0.6 56,561 - GFF4420 0.26 +0.4 56,561 - GFF4420 0.26 +0.0 56,860 + GFF4420 0.48 -0.1 56,860 + GFF4420 0.48 +0.5 56,860 + GFF4420 0.48 -0.8 56,861 - GFF4420 0.48 -1.1 56,861 - GFF4420 0.48 +0.0 57,579 + -1.2 57,579 + +0.1 57,579 + +0.7 57,579 + +0.3 57,580 - -0.7 57,580 - -0.2 57,580 - +0.3 57,580 - +2.8 57,580 - +0.2 57,580 - +0.3 57,622 - -0.8 57,651 + -0.5 57,651 + -1.2 57,652 - -0.3 57,652 - -0.2 57,652 - +0.2 57,670 + GFF4421 0.11 -0.8 57,670 + GFF4421 0.11 -0.4 57,670 + GFF4421 0.11 +0.5 57,670 + GFF4421 0.11 -0.4 57,670 + GFF4421 0.11 -0.0 57,670 + GFF4421 0.11 -1.1 57,671 - GFF4421 0.11 -0.9 57,671 - GFF4421 0.11 -0.2 57,671 - GFF4421 0.11 +0.8 57,671 - GFF4421 0.11 +0.4 57,671 - GFF4421 0.11 -0.4 57,671 - GFF4421 0.11 +0.1 57,671 - GFF4421 0.11 -0.7 57,804 + GFF4421 0.30 +0.2 57,804 + GFF4421 0.30 -0.3 57,804 + GFF4421 0.30 -0.2 57,804 + GFF4421 0.30 -0.2 57,804 + GFF4421 0.30 -0.2 57,804 + GFF4421 0.30 +0.0 57,804 + GFF4421 0.30 -0.4 57,804 + GFF4421 0.30 +1.3 57,804 + GFF4421 0.30 -0.3 57,804 + GFF4421 0.30 -2.0 57,804 + GFF4421 0.30 -0.7 57,805 - GFF4421 0.30 +0.6 57,805 - GFF4421 0.30 -0.2 57,805 - GFF4421 0.30 -0.1 57,805 - GFF4421 0.30 +0.5 57,805 - GFF4421 0.30 -1.9 57,805 - GFF4421 0.30 -0.9 57,805 - GFF4421 0.30 +0.2 57,805 - GFF4421 0.30 +1.1 57,805 - GFF4421 0.30 -0.2 57,805 - GFF4421 0.30 -0.8 57,864 + GFF4421 0.38 +0.1 57,972 + GFF4421 0.54 -1.0 57,973 - GFF4421 0.54 +0.8 57,978 + GFF4421 0.54 -0.6 57,978 + GFF4421 0.54 -0.3 57,979 - GFF4421 0.55 -1.1 57,979 - GFF4421 0.55 +0.3 58,014 + GFF4421 0.60 -0.5 58,015 - GFF4421 0.60 -0.6 58,015 - GFF4421 0.60 +0.8 58,131 + GFF4421 0.76 -1.8 58,131 + GFF4421 0.76 -1.0 58,239 + -1.4 58,239 + +0.5 58,239 + +0.4 58,239 + -0.1 58,239 + -2.1 58,239 + +1.0 58,239 + -1.4 58,239 + -0.2 58,239 + +0.3 58,239 + +0.2 58,239 + +1.5 58,239 + -0.1 58,239 + -1.5 58,240 - -0.3 58,240 - -0.4 58,240 - +0.1 58,240 - -1.1 58,240 - -0.0 58,240 - -0.4 58,240 - -0.4 58,240 - -1.4 58,240 - +1.3 58,473 + GFF4422 0.18 -1.4 58,473 + GFF4422 0.18 +0.5 58,473 + GFF4422 0.18 +1.1 58,473 + GFF4422 0.18 -1.5 58,473 + GFF4422 0.18 -0.1 58,473 + GFF4422 0.18 -1.5 58,474 - GFF4422 0.18 -1.0 58,474 - GFF4422 0.18 -0.7 58,474 - GFF4422 0.18 -1.8 58,474 - GFF4422 0.18 -0.6 58,474 - GFF4422 0.18 -0.5 58,474 - GFF4422 0.18 +0.4
Or see this region's nucleotide sequence