Strain Fitness in Variovorax sp. SCN45 around GFF4322

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4321 and GFF4322 are separated by 159 nucleotidesGFF4322 and GFF4323 are separated by 14 nucleotidesGFF4323 and GFF4324 are separated by 26 nucleotides GFF4321 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 242,706 to 244,262 GFF4321 GFF4322 - Acyl-CoA dehydrogenase, at 244,422 to 245,582 GFF4322 GFF4323 - no description, at 245,597 to 246,220 GFF4323 GFF4324 - no description, at 246,247 to 247,281 GFF4324 Position (kb) 244 245 246Strain fitness (log2 ratio) -2 -1 0 1at 243.510 kb on + strand, within GFF4321at 243.510 kb on + strand, within GFF4321at 243.511 kb on - strand, within GFF4321at 243.618 kb on + strand, within GFF4321at 243.915 kb on + strand, within GFF4321at 243.916 kb on - strand, within GFF4321at 243.966 kb on + strand, within GFF4321at 243.967 kb on - strand, within GFF4321at 244.144 kb on - strandat 244.400 kb on - strandat 244.854 kb on + strand, within GFF4322at 245.202 kb on + strand, within GFF4322at 245.565 kb on + strandat 245.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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243,510 + GFF4321 0.52 +0.9
243,510 + GFF4321 0.52 +0.5
243,511 - GFF4321 0.52 +0.9
243,618 + GFF4321 0.59 -1.5
243,915 + GFF4321 0.78 -0.3
243,916 - GFF4321 0.78 -1.0
243,966 + GFF4321 0.81 -1.9
243,967 - GFF4321 0.81 -0.0
244,144 - +0.6
244,400 - -0.5
244,854 + GFF4322 0.37 +0.0
245,202 + GFF4322 0.67 +0.1
245,565 + -0.2
245,565 + -0.9

Or see this region's nucleotide sequence