Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4316 and GFF4317 are separated by 4 nucleotides GFF4317 and GFF4318 are separated by 21 nucleotides GFF4318 and GFF4319 are separated by 25 nucleotides GFF4319 and GFF4320 are separated by 187 nucleotides
GFF4316 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 236,525 to 238,327
GFF4316
GFF4317 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 238,332 to 239,210
GFF4317
GFF4318 - no description, at 239,232 to 240,428
GFF4318
GFF4319 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 240,454 to 241,215
GFF4319
GFF4320 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 241,403 to 242,620
GFF4320
Position (kb) 239
240
241 Strain fitness (log2 ratio) -2
-1
0
1 at 238.333 kb on + strand at 238.786 kb on + strand, within GFF4317 at 239.164 kb on + strand at 239.453 kb on - strand, within GFF4318 at 239.459 kb on - strand, within GFF4318 at 239.459 kb on - strand, within GFF4318 at 239.579 kb on + strand, within GFF4318 at 239.593 kb on + strand, within GFF4318 at 239.779 kb on + strand, within GFF4318 at 239.779 kb on + strand, within GFF4318 at 239.810 kb on - strand, within GFF4318 at 239.977 kb on + strand, within GFF4318 at 239.977 kb on + strand, within GFF4318 at 240.001 kb on + strand, within GFF4318 at 240.265 kb on + strand, within GFF4318 at 240.266 kb on - strand, within GFF4318 at 240.459 kb on - strand at 240.747 kb on - strand, within GFF4319 at 241.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 238,333 + +0.9 238,786 + GFF4317 0.52 -0.3 239,164 + -0.5 239,453 - GFF4318 0.18 +0.5 239,459 - GFF4318 0.19 -1.3 239,459 - GFF4318 0.19 +0.2 239,579 + GFF4318 0.29 -0.5 239,593 + GFF4318 0.30 +0.4 239,779 + GFF4318 0.46 -0.8 239,779 + GFF4318 0.46 +0.0 239,810 - GFF4318 0.48 -1.2 239,977 + GFF4318 0.62 -0.8 239,977 + GFF4318 0.62 -1.9 240,001 + GFF4318 0.64 -1.5 240,265 + GFF4318 0.86 -0.8 240,266 - GFF4318 0.86 -0.6 240,459 - +1.2 240,747 - GFF4319 0.38 -1.0 241,348 + +0.1
Or see this region's nucleotide sequence