Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4253 and GFF4254 are separated by 152 nucleotides GFF4254 and GFF4255 overlap by 4 nucleotides GFF4255 and GFF4256 are separated by 112 nucleotides
GFF4253 - hypothetical protein, at 168,861 to 169,838
GFF4253
GFF4254 - hypothetical protein, at 169,991 to 170,362
GFF4254
GFF4255 - no description, at 170,359 to 171,402
GFF4255
GFF4256 - Vanillate O-demethylase oxygenase subunit (EC 1.14.13.82), at 171,515 to 172,588
GFF4256
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1 at 169.596 kb on + strand, within GFF4253 at 169.597 kb on - strand, within GFF4253 at 169.597 kb on - strand, within GFF4253 at 169.764 kb on + strand at 169.803 kb on + strand at 169.804 kb on - strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.926 kb on + strand at 169.927 kb on - strand at 169.927 kb on - strand at 169.927 kb on - strand at 170.156 kb on + strand, within GFF4254 at 170.270 kb on + strand, within GFF4254 at 170.271 kb on - strand, within GFF4254 at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.360 kb on + strand at 170.361 kb on - strand at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.870 kb on + strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.871 kb on - strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.882 kb on + strand, within GFF4255 at 170.927 kb on + strand, within GFF4255 at 170.944 kb on + strand, within GFF4255 at 170.945 kb on - strand, within GFF4255 at 171.406 kb on + strand at 171.406 kb on + strand at 171.448 kb on + strand at 171.453 kb on - strand at 171.536 kb on + strand at 171.615 kb on - strand at 171.720 kb on - strand, within GFF4256 at 171.917 kb on + strand, within GFF4256 at 171.917 kb on + strand, within GFF4256 at 171.995 kb on + strand, within GFF4256 at 171.995 kb on + strand, within GFF4256 at 171.995 kb on + strand, within GFF4256 at 171.996 kb on - strand, within GFF4256 at 171.996 kb on - strand, within GFF4256 at 171.996 kb on - strand, within GFF4256 at 172.092 kb on + strand, within GFF4256 at 172.304 kb on + strand, within GFF4256 at 172.304 kb on + strand, within GFF4256 at 172.364 kb on + strand, within GFF4256 at 172.365 kb on - strand, within GFF4256
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 169,596 + GFF4253 0.75 -0.1 169,597 - GFF4253 0.75 -0.4 169,597 - GFF4253 0.75 -0.3 169,764 + -1.2 169,803 + -0.7 169,804 - +0.5 169,926 + +0.7 169,926 + +0.2 169,926 + -0.3 169,926 + -1.0 169,927 - +0.4 169,927 - +0.2 169,927 - -1.1 170,156 + GFF4254 0.44 +1.3 170,270 + GFF4254 0.75 +0.9 170,271 - GFF4254 0.75 +0.9 170,360 + +0.3 170,360 + -0.1 170,360 + +1.7 170,360 + -0.6 170,360 + +0.4 170,361 - -1.6 170,870 + GFF4255 0.49 -0.1 170,870 + GFF4255 0.49 -1.6 170,870 + GFF4255 0.49 -0.0 170,870 + GFF4255 0.49 -1.0 170,871 - GFF4255 0.49 +0.2 170,871 - GFF4255 0.49 -0.3 170,871 - GFF4255 0.49 -0.9 170,882 + GFF4255 0.50 -0.3 170,882 + GFF4255 0.50 +0.7 170,882 + GFF4255 0.50 -0.1 170,882 + GFF4255 0.50 -0.9 170,927 + GFF4255 0.54 -0.7 170,944 + GFF4255 0.56 -0.0 170,945 - GFF4255 0.56 -0.5 171,406 + -2.5 171,406 + -0.0 171,448 + -0.8 171,453 - -0.7 171,536 + -0.3 171,615 - -1.2 171,720 - GFF4256 0.19 +0.2 171,917 + GFF4256 0.37 -0.0 171,917 + GFF4256 0.37 -0.6 171,995 + GFF4256 0.45 +0.4 171,995 + GFF4256 0.45 -1.5 171,995 + GFF4256 0.45 -0.5 171,996 - GFF4256 0.45 -0.2 171,996 - GFF4256 0.45 -1.5 171,996 - GFF4256 0.45 +0.0 172,092 + GFF4256 0.54 -0.2 172,304 + GFF4256 0.73 +0.6 172,304 + GFF4256 0.73 +0.5 172,364 + GFF4256 0.79 -0.7 172,365 - GFF4256 0.79 +0.2
Or see this region's nucleotide sequence