Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4177 and GFF4178 are separated by 11 nucleotides GFF4178 and GFF4179 are separated by 57 nucleotides GFF4179 and GFF4180 are separated by 192 nucleotides
GFF4177 - Epoxyqueuosine reductase (EC 1.17.99.6) QueH, at 81,936 to 82,160
GFF4177
GFF4178 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 82,172 to 83,029
GFF4178
GFF4179 - hypothetical protein, at 83,087 to 83,692
GFF4179
GFF4180 - hypothetical protein, at 83,885 to 86,224
GFF4180
Position (kb)
83
84 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 82.206 kb on - strand at 82.284 kb on - strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.785 kb on + strand, within GFF4178 at 82.786 kb on - strand, within GFF4178 at 82.796 kb on + strand, within GFF4178 at 82.844 kb on + strand, within GFF4178 at 82.989 kb on + strand at 82.989 kb on + strand at 83.089 kb on - strand at 83.089 kb on - strand at 83.214 kb on + strand, within GFF4179 at 83.216 kb on + strand, within GFF4179 at 83.216 kb on + strand, within GFF4179 at 83.216 kb on + strand, within GFF4179 at 83.217 kb on - strand, within GFF4179 at 83.361 kb on + strand, within GFF4179 at 83.361 kb on + strand, within GFF4179 at 83.361 kb on + strand, within GFF4179 at 83.412 kb on + strand, within GFF4179 at 83.412 kb on + strand, within GFF4179 at 83.451 kb on + strand, within GFF4179 at 83.452 kb on - strand, within GFF4179 at 83.460 kb on + strand, within GFF4179 at 83.460 kb on + strand, within GFF4179 at 83.461 kb on - strand, within GFF4179 at 83.500 kb on - strand, within GFF4179 at 83.514 kb on + strand, within GFF4179 at 83.514 kb on + strand, within GFF4179 at 83.514 kb on + strand, within GFF4179 at 83.515 kb on - strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.613 kb on + strand, within GFF4179 at 83.614 kb on - strand, within GFF4179 at 83.614 kb on - strand, within GFF4179 at 83.614 kb on - strand, within GFF4179 at 83.634 kb on + strand at 83.634 kb on + strand at 83.634 kb on + strand at 83.634 kb on + strand at 83.634 kb on + strand at 83.634 kb on + strand at 83.635 kb on - strand at 83.635 kb on - strand at 83.635 kb on - strand at 83.635 kb on - strand at 83.635 kb on - strand at 83.635 kb on - strand at 83.674 kb on + strand at 83.674 kb on + strand at 83.675 kb on - strand at 83.887 kb on - strand at 83.963 kb on + strand at 84.048 kb on + strand at 84.048 kb on + strand at 84.049 kb on - strand at 84.049 kb on - strand at 84.064 kb on + strand at 84.250 kb on + strand, within GFF4180 at 84.250 kb on + strand, within GFF4180 at 84.251 kb on - strand, within GFF4180 at 84.251 kb on - strand, within GFF4180 at 84.569 kb on + strand, within GFF4180 at 84.569 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.612 kb on + strand, within GFF4180 at 84.613 kb on - strand, within GFF4180 at 84.613 kb on - strand, within GFF4180 at 84.613 kb on - strand, within GFF4180 at 84.613 kb on - strand, within GFF4180 at 84.613 kb on - strand, within GFF4180 at 84.613 kb on - strand, within GFF4180
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 82,206 - -0.0 82,284 - GFF4178 0.13 +0.2 82,292 + GFF4178 0.14 +0.6 82,292 + GFF4178 0.14 -0.6 82,701 + GFF4178 0.62 -0.2 82,701 + GFF4178 0.62 +1.0 82,701 + GFF4178 0.62 +0.2 82,701 + GFF4178 0.62 +0.7 82,702 - GFF4178 0.62 +0.7 82,702 - GFF4178 0.62 +0.1 82,785 + GFF4178 0.71 -1.0 82,786 - GFF4178 0.72 -0.0 82,796 + GFF4178 0.73 +0.5 82,844 + GFF4178 0.78 -0.3 82,989 + +0.4 82,989 + -0.1 83,089 - +0.6 83,089 - -1.3 83,214 + GFF4179 0.21 -1.0 83,216 + GFF4179 0.21 -0.1 83,216 + GFF4179 0.21 +0.3 83,216 + GFF4179 0.21 -0.6 83,217 - GFF4179 0.21 -0.6 83,361 + GFF4179 0.45 +1.4 83,361 + GFF4179 0.45 +0.9 83,361 + GFF4179 0.45 -1.6 83,412 + GFF4179 0.54 +1.1 83,412 + GFF4179 0.54 -0.2 83,451 + GFF4179 0.60 -0.5 83,452 - GFF4179 0.60 +0.4 83,460 + GFF4179 0.62 +1.3 83,460 + GFF4179 0.62 +0.5 83,461 - GFF4179 0.62 +1.0 83,500 - GFF4179 0.68 -0.1 83,514 + GFF4179 0.70 +0.0 83,514 + GFF4179 0.70 +0.5 83,514 + GFF4179 0.70 +0.2 83,515 - GFF4179 0.71 -0.2 83,613 + GFF4179 0.87 +0.8 83,613 + GFF4179 0.87 +0.5 83,613 + GFF4179 0.87 +1.3 83,613 + GFF4179 0.87 -0.2 83,613 + GFF4179 0.87 +0.9 83,613 + GFF4179 0.87 -0.3 83,613 + GFF4179 0.87 -0.3 83,614 - GFF4179 0.87 +0.1 83,614 - GFF4179 0.87 -0.7 83,614 - GFF4179 0.87 -1.8 83,634 + -0.3 83,634 + +0.4 83,634 + +1.3 83,634 + -0.2 83,634 + -1.2 83,634 + -0.4 83,635 - -0.0 83,635 - -0.9 83,635 - -1.6 83,635 - +0.5 83,635 - -0.6 83,635 - +1.7 83,674 + -0.5 83,674 + -0.8 83,675 - -1.1 83,887 - -2.4 83,963 + +1.3 84,048 + +0.3 84,048 + -1.5 84,049 - +0.2 84,049 - -0.1 84,064 + +0.5 84,250 + GFF4180 0.16 +0.3 84,250 + GFF4180 0.16 -0.8 84,251 - GFF4180 0.16 +0.7 84,251 - GFF4180 0.16 -0.0 84,569 + GFF4180 0.29 -1.1 84,569 + GFF4180 0.29 -0.3 84,612 + GFF4180 0.31 +0.1 84,612 + GFF4180 0.31 -0.9 84,612 + GFF4180 0.31 +0.7 84,612 + GFF4180 0.31 -1.8 84,612 + GFF4180 0.31 +2.3 84,612 + GFF4180 0.31 -0.8 84,612 + GFF4180 0.31 +1.7 84,613 - GFF4180 0.31 +0.2 84,613 - GFF4180 0.31 +0.1 84,613 - GFF4180 0.31 -0.7 84,613 - GFF4180 0.31 -0.3 84,613 - GFF4180 0.31 -0.8 84,613 - GFF4180 0.31 -0.4
Or see this region's nucleotide sequence