Strain Fitness in Variovorax sp. SCN45 around GFF4178

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4176 and GFF4177 overlap by 4 nucleotidesGFF4177 and GFF4178 are separated by 11 nucleotidesGFF4178 and GFF4179 are separated by 57 nucleotidesGFF4179 and GFF4180 are separated by 192 nucleotides GFF4176 - Transcriptional activator MetR, at 81,040 to 81,939 GFF4176 GFF4177 - Epoxyqueuosine reductase (EC 1.17.99.6) QueH, at 81,936 to 82,160 GFF4177 GFF4178 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 82,172 to 83,029 GFF4178 GFF4179 - hypothetical protein, at 83,087 to 83,692 GFF4179 GFF4180 - hypothetical protein, at 83,885 to 86,224 GFF4180 Position (kb) 82 83 84Strain fitness (log2 ratio) -2 -1 0 1 2at 81.230 kb on - strand, within GFF4176at 81.349 kb on + strand, within GFF4176at 81.349 kb on + strand, within GFF4176at 81.529 kb on + strand, within GFF4176at 81.529 kb on + strand, within GFF4176at 81.530 kb on - strand, within GFF4176at 81.581 kb on - strand, within GFF4176at 81.623 kb on - strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.652 kb on + strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.653 kb on - strand, within GFF4176at 81.752 kb on + strand, within GFF4176at 81.784 kb on + strand, within GFF4176at 81.784 kb on + strand, within GFF4176at 81.883 kb on + strandat 81.883 kb on + strandat 81.884 kb on - strandat 81.966 kb on + strand, within GFF4177at 81.966 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.978 kb on + strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 81.979 kb on - strand, within GFF4177at 82.065 kb on + strand, within GFF4177at 82.066 kb on - strand, within GFF4177at 82.066 kb on - strand, within GFF4177at 82.206 kb on - strandat 82.284 kb on - strand, within GFF4178at 82.292 kb on + strand, within GFF4178at 82.292 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.701 kb on + strand, within GFF4178at 82.702 kb on - strand, within GFF4178at 82.702 kb on - strand, within GFF4178at 82.785 kb on + strand, within GFF4178at 82.786 kb on - strand, within GFF4178at 82.796 kb on + strand, within GFF4178at 82.844 kb on + strand, within GFF4178at 82.989 kb on + strandat 82.989 kb on + strandat 83.089 kb on - strandat 83.089 kb on - strandat 83.214 kb on + strand, within GFF4179at 83.216 kb on + strand, within GFF4179at 83.216 kb on + strand, within GFF4179at 83.216 kb on + strand, within GFF4179at 83.217 kb on - strand, within GFF4179at 83.361 kb on + strand, within GFF4179at 83.361 kb on + strand, within GFF4179at 83.361 kb on + strand, within GFF4179at 83.412 kb on + strand, within GFF4179at 83.412 kb on + strand, within GFF4179at 83.451 kb on + strand, within GFF4179at 83.452 kb on - strand, within GFF4179at 83.460 kb on + strand, within GFF4179at 83.460 kb on + strand, within GFF4179at 83.461 kb on - strand, within GFF4179at 83.500 kb on - strand, within GFF4179at 83.514 kb on + strand, within GFF4179at 83.514 kb on + strand, within GFF4179at 83.514 kb on + strand, within GFF4179at 83.515 kb on - strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.613 kb on + strand, within GFF4179at 83.614 kb on - strand, within GFF4179at 83.614 kb on - strand, within GFF4179at 83.614 kb on - strand, within GFF4179at 83.634 kb on + strandat 83.634 kb on + strandat 83.634 kb on + strandat 83.634 kb on + strandat 83.634 kb on + strandat 83.634 kb on + strandat 83.635 kb on - strandat 83.635 kb on - strandat 83.635 kb on - strandat 83.635 kb on - strandat 83.635 kb on - strandat 83.635 kb on - strandat 83.674 kb on + strandat 83.674 kb on + strandat 83.675 kb on - strandat 83.887 kb on - strandat 83.963 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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81,230 - GFF4176 0.21 +1.3
81,349 + GFF4176 0.34 -2.1
81,349 + GFF4176 0.34 +2.7
81,529 + GFF4176 0.54 -0.4
81,529 + GFF4176 0.54 +0.7
81,530 - GFF4176 0.54 -0.7
81,581 - GFF4176 0.60 +0.7
81,623 - GFF4176 0.65 +0.2
81,652 + GFF4176 0.68 -0.8
81,652 + GFF4176 0.68 -0.0
81,652 + GFF4176 0.68 +1.1
81,652 + GFF4176 0.68 +0.2
81,652 + GFF4176 0.68 -0.6
81,652 + GFF4176 0.68 +0.1
81,652 + GFF4176 0.68 -0.2
81,652 + GFF4176 0.68 +0.4
81,652 + GFF4176 0.68 -2.5
81,652 + GFF4176 0.68 +0.7
81,652 + GFF4176 0.68 -0.3
81,652 + GFF4176 0.68 -0.5
81,652 + GFF4176 0.68 +0.2
81,652 + GFF4176 0.68 -0.5
81,653 - GFF4176 0.68 -0.1
81,653 - GFF4176 0.68 -0.2
81,653 - GFF4176 0.68 -0.0
81,653 - GFF4176 0.68 -0.4
81,653 - GFF4176 0.68 -0.1
81,653 - GFF4176 0.68 +0.2
81,653 - GFF4176 0.68 -0.1
81,653 - GFF4176 0.68 -0.6
81,653 - GFF4176 0.68 +1.2
81,653 - GFF4176 0.68 +0.2
81,653 - GFF4176 0.68 -0.1
81,653 - GFF4176 0.68 -2.0
81,653 - GFF4176 0.68 -0.9
81,653 - GFF4176 0.68 +0.4
81,752 + GFF4176 0.79 -0.5
81,784 + GFF4176 0.83 +0.6
81,784 + GFF4176 0.83 +0.5
81,883 + +0.0
81,883 + +0.2
81,884 - -0.0
81,966 + GFF4177 0.13 -0.2
81,966 + GFF4177 0.13 +0.1
81,978 + GFF4177 0.19 +2.5
81,978 + GFF4177 0.19 +1.7
81,978 + GFF4177 0.19 -0.3
81,979 - GFF4177 0.19 -0.9
81,979 - GFF4177 0.19 -0.7
81,979 - GFF4177 0.19 -1.1
82,065 + GFF4177 0.57 +0.4
82,066 - GFF4177 0.58 +0.7
82,066 - GFF4177 0.58 +1.2
82,206 - -0.0
82,284 - GFF4178 0.13 +0.2
82,292 + GFF4178 0.14 +0.6
82,292 + GFF4178 0.14 -0.6
82,701 + GFF4178 0.62 -0.2
82,701 + GFF4178 0.62 +1.0
82,701 + GFF4178 0.62 +0.2
82,701 + GFF4178 0.62 +0.7
82,702 - GFF4178 0.62 +0.7
82,702 - GFF4178 0.62 +0.1
82,785 + GFF4178 0.71 -1.0
82,786 - GFF4178 0.72 -0.0
82,796 + GFF4178 0.73 +0.5
82,844 + GFF4178 0.78 -0.3
82,989 + +0.4
82,989 + -0.1
83,089 - +0.6
83,089 - -1.3
83,214 + GFF4179 0.21 -1.0
83,216 + GFF4179 0.21 -0.1
83,216 + GFF4179 0.21 +0.3
83,216 + GFF4179 0.21 -0.6
83,217 - GFF4179 0.21 -0.6
83,361 + GFF4179 0.45 +1.4
83,361 + GFF4179 0.45 +0.9
83,361 + GFF4179 0.45 -1.6
83,412 + GFF4179 0.54 +1.1
83,412 + GFF4179 0.54 -0.2
83,451 + GFF4179 0.60 -0.5
83,452 - GFF4179 0.60 +0.4
83,460 + GFF4179 0.62 +1.3
83,460 + GFF4179 0.62 +0.5
83,461 - GFF4179 0.62 +1.0
83,500 - GFF4179 0.68 -0.1
83,514 + GFF4179 0.70 +0.0
83,514 + GFF4179 0.70 +0.5
83,514 + GFF4179 0.70 +0.2
83,515 - GFF4179 0.71 -0.2
83,613 + GFF4179 0.87 +0.8
83,613 + GFF4179 0.87 +0.5
83,613 + GFF4179 0.87 +1.3
83,613 + GFF4179 0.87 -0.2
83,613 + GFF4179 0.87 +0.9
83,613 + GFF4179 0.87 -0.3
83,613 + GFF4179 0.87 -0.3
83,614 - GFF4179 0.87 +0.1
83,614 - GFF4179 0.87 -0.7
83,614 - GFF4179 0.87 -1.8
83,634 + -0.3
83,634 + +0.4
83,634 + +1.3
83,634 + -0.2
83,634 + -1.2
83,634 + -0.4
83,635 - -0.0
83,635 - -0.9
83,635 - -1.6
83,635 - +0.5
83,635 - -0.6
83,635 - +1.7
83,674 + -0.5
83,674 + -0.8
83,675 - -1.1
83,887 - -2.4
83,963 + +1.3

Or see this region's nucleotide sequence