Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4170 and GFF4171 are separated by 34 nucleotides GFF4171 and GFF4172 are separated by 131 nucleotides GFF4172 and GFF4173 are separated by 97 nucleotides
GFF4170 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 74,898 to 76,454
GFF4170
GFF4171 - hypothetical protein, at 76,489 to 77,337
GFF4171
GFF4172 - no description, at 77,469 to 78,149
GFF4172
GFF4173 - 5-methyltetrahydropteroyltriglutamate--homocystei ne methyltransferase (EC 2.1.1.14), at 78,247 to 79,272
GFF4173
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 75.529 kb on + strand, within GFF4170 at 76.114 kb on + strand, within GFF4170 at 76.114 kb on + strand, within GFF4170 at 76.115 kb on - strand, within GFF4170 at 76.198 kb on + strand, within GFF4170 at 76.198 kb on + strand, within GFF4170 at 76.199 kb on - strand, within GFF4170 at 76.199 kb on - strand, within GFF4170 at 76.199 kb on - strand, within GFF4170 at 77.063 kb on + strand, within GFF4171 at 77.063 kb on + strand, within GFF4171 at 77.063 kb on + strand, within GFF4171 at 77.063 kb on + strand, within GFF4171 at 77.063 kb on + strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.064 kb on - strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.069 kb on + strand, within GFF4171 at 77.070 kb on - strand, within GFF4171 at 77.070 kb on - strand, within GFF4171 at 77.070 kb on - strand, within GFF4171 at 77.070 kb on - strand, within GFF4171 at 77.126 kb on - strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.252 kb on + strand, within GFF4171 at 77.253 kb on - strand at 77.253 kb on - strand at 77.253 kb on - strand at 77.253 kb on - strand at 77.253 kb on - strand at 77.261 kb on + strand at 77.261 kb on + strand at 77.261 kb on + strand at 77.261 kb on + strand at 77.262 kb on - strand at 77.262 kb on - strand at 77.262 kb on - strand at 77.262 kb on - strand at 77.262 kb on - strand at 77.291 kb on + strand at 77.291 kb on + strand at 77.291 kb on + strand at 77.291 kb on + strand at 77.291 kb on + strand at 77.292 kb on - strand at 77.292 kb on - strand at 77.292 kb on - strand at 77.292 kb on - strand at 77.292 kb on - strand at 77.292 kb on - strand at 77.386 kb on + strand at 77.392 kb on + strand at 77.392 kb on + strand at 77.451 kb on + strand at 77.451 kb on + strand at 77.514 kb on - strand at 77.514 kb on - strand at 77.643 kb on + strand, within GFF4172 at 77.728 kb on - strand, within GFF4172 at 77.728 kb on - strand, within GFF4172 at 77.728 kb on - strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on + strand, within GFF4172 at 77.730 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 77.731 kb on - strand, within GFF4172 at 78.125 kb on + strand at 78.128 kb on + strand at 78.192 kb on + strand at 78.192 kb on + strand at 78.193 kb on - strand at 78.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 75,529 + GFF4170 0.41 +0.0 76,114 + GFF4170 0.78 -0.8 76,114 + GFF4170 0.78 -0.5 76,115 - GFF4170 0.78 -0.3 76,198 + GFF4170 0.83 -0.3 76,198 + GFF4170 0.83 +0.4 76,199 - GFF4170 0.84 -1.0 76,199 - GFF4170 0.84 -0.8 76,199 - GFF4170 0.84 +1.4 77,063 + GFF4171 0.68 +1.0 77,063 + GFF4171 0.68 -0.4 77,063 + GFF4171 0.68 +0.2 77,063 + GFF4171 0.68 -1.2 77,063 + GFF4171 0.68 -0.6 77,064 - GFF4171 0.68 -0.6 77,064 - GFF4171 0.68 +0.2 77,064 - GFF4171 0.68 +0.4 77,064 - GFF4171 0.68 -0.3 77,064 - GFF4171 0.68 -2.0 77,064 - GFF4171 0.68 -0.2 77,069 + GFF4171 0.68 -0.3 77,069 + GFF4171 0.68 +3.3 77,069 + GFF4171 0.68 -0.1 77,069 + GFF4171 0.68 -0.4 77,069 + GFF4171 0.68 +0.1 77,069 + GFF4171 0.68 -1.4 77,070 - GFF4171 0.68 +0.2 77,070 - GFF4171 0.68 +1.4 77,070 - GFF4171 0.68 +0.2 77,070 - GFF4171 0.68 +2.3 77,126 - GFF4171 0.75 +0.1 77,252 + GFF4171 0.90 -0.3 77,252 + GFF4171 0.90 +0.7 77,252 + GFF4171 0.90 +0.2 77,252 + GFF4171 0.90 -0.7 77,252 + GFF4171 0.90 -0.9 77,252 + GFF4171 0.90 +0.6 77,252 + GFF4171 0.90 +0.1 77,252 + GFF4171 0.90 +0.6 77,252 + GFF4171 0.90 -0.7 77,252 + GFF4171 0.90 +0.5 77,252 + GFF4171 0.90 -0.6 77,253 - -0.5 77,253 - -0.6 77,253 - -0.8 77,253 - +0.1 77,253 - +1.0 77,261 + +0.3 77,261 + +0.5 77,261 + +0.4 77,261 + +0.3 77,262 - +0.6 77,262 - -2.5 77,262 - -1.2 77,262 - +0.1 77,262 - +1.3 77,291 + -0.2 77,291 + +0.3 77,291 + +0.1 77,291 + +0.0 77,291 + +0.3 77,292 - +0.2 77,292 - -0.4 77,292 - +0.2 77,292 - -0.0 77,292 - -0.4 77,292 - -0.3 77,386 + -0.6 77,392 + -0.3 77,392 + +1.1 77,451 + +0.0 77,451 + +0.1 77,514 - +0.9 77,514 - -0.0 77,643 + GFF4172 0.26 -1.2 77,728 - GFF4172 0.38 +0.1 77,728 - GFF4172 0.38 -0.6 77,728 - GFF4172 0.38 -0.3 77,730 + GFF4172 0.38 +1.4 77,730 + GFF4172 0.38 -0.8 77,730 + GFF4172 0.38 -0.9 77,730 + GFF4172 0.38 -0.0 77,730 + GFF4172 0.38 +0.7 77,730 + GFF4172 0.38 -0.9 77,730 + GFF4172 0.38 +0.1 77,730 + GFF4172 0.38 -0.4 77,730 + GFF4172 0.38 -0.4 77,730 + GFF4172 0.38 -1.6 77,730 + GFF4172 0.38 -2.8 77,730 + GFF4172 0.38 -0.1 77,730 - GFF4172 0.38 -0.2 77,731 - GFF4172 0.38 -1.0 77,731 - GFF4172 0.38 +0.3 77,731 - GFF4172 0.38 -1.1 77,731 - GFF4172 0.38 +0.0 77,731 - GFF4172 0.38 -0.9 77,731 - GFF4172 0.38 -0.9 78,125 + +1.7 78,128 + +0.8 78,192 + +0.3 78,192 + +0.9 78,193 - -0.6 78,193 - -0.6
Or see this region's nucleotide sequence