Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4146 and GFF4147 overlap by 17 nucleotides GFF4147 and GFF4148 are separated by 147 nucleotides GFF4148 and GFF4149 are separated by 91 nucleotides
GFF4146 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 52,737 to 53,672
GFF4146
GFF4147 - hypothetical protein, at 53,656 to 53,946
GFF4147
GFF4148 - hypothetical protein, at 54,094 to 55,098
GFF4148
GFF4149 - hypothetical protein, at 55,190 to 56,152
GFF4149
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 53.253 kb on + strand, within GFF4146 at 53.724 kb on - strand, within GFF4147 at 53.801 kb on + strand, within GFF4147 at 53.801 kb on + strand, within GFF4147 at 53.802 kb on - strand, within GFF4147 at 53.808 kb on - strand, within GFF4147 at 53.808 kb on - strand, within GFF4147 at 53.883 kb on - strand, within GFF4147 at 53.919 kb on - strand at 53.982 kb on + strand at 53.982 kb on + strand at 53.983 kb on - strand at 53.985 kb on - strand at 54.002 kb on + strand at 54.003 kb on - strand at 54.024 kb on + strand at 54.024 kb on + strand at 54.025 kb on - strand at 54.146 kb on + strand at 54.146 kb on + strand at 54.146 kb on + strand at 54.147 kb on - strand at 54.570 kb on - strand, within GFF4148 at 54.601 kb on - strand, within GFF4148 at 54.834 kb on - strand, within GFF4148 at 54.834 kb on - strand, within GFF4148 at 54.900 kb on - strand, within GFF4148 at 54.942 kb on - strand, within GFF4148 at 54.942 kb on - strand, within GFF4148 at 54.942 kb on - strand, within GFF4148 at 54.950 kb on + strand, within GFF4148 at 54.950 kb on + strand, within GFF4148 at 54.951 kb on - strand, within GFF4148 at 54.951 kb on - strand, within GFF4148 at 54.951 kb on - strand, within GFF4148 at 55.162 kb on + strand at 55.422 kb on + strand, within GFF4149 at 55.423 kb on - strand, within GFF4149 at 55.430 kb on + strand, within GFF4149 at 55.431 kb on - strand, within GFF4149 at 55.431 kb on - strand, within GFF4149
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 53,253 + GFF4146 0.55 -0.9 53,724 - GFF4147 0.23 -0.6 53,801 + GFF4147 0.50 +0.7 53,801 + GFF4147 0.50 +1.0 53,802 - GFF4147 0.50 +0.5 53,808 - GFF4147 0.52 -0.6 53,808 - GFF4147 0.52 -1.8 53,883 - GFF4147 0.78 -0.1 53,919 - -1.5 53,982 + +1.4 53,982 + +1.7 53,983 - +0.0 53,985 - +0.4 54,002 + +0.1 54,003 - +0.1 54,024 + -0.7 54,024 + +2.1 54,025 - -0.1 54,146 + -0.5 54,146 + -2.2 54,146 + -0.4 54,147 - -3.3 54,570 - GFF4148 0.47 +1.7 54,601 - GFF4148 0.50 -1.2 54,834 - GFF4148 0.74 -0.1 54,834 - GFF4148 0.74 -0.5 54,900 - GFF4148 0.80 -0.0 54,942 - GFF4148 0.84 +0.1 54,942 - GFF4148 0.84 -1.2 54,942 - GFF4148 0.84 -0.1 54,950 + GFF4148 0.85 -1.1 54,950 + GFF4148 0.85 -0.8 54,951 - GFF4148 0.85 -1.8 54,951 - GFF4148 0.85 -0.5 54,951 - GFF4148 0.85 +0.4 55,162 + +0.3 55,422 + GFF4149 0.24 +0.4 55,423 - GFF4149 0.24 -0.1 55,430 + GFF4149 0.25 -0.2 55,431 - GFF4149 0.25 -0.6 55,431 - GFF4149 0.25 +0.3
Or see this region's nucleotide sequence