Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4025 and GFF4026 are separated by 10 nucleotides GFF4026 and GFF4027 overlap by 4 nucleotides GFF4027 and GFF4028 are separated by 165 nucleotides GFF4028 and GFF4029 are separated by 129 nucleotides
GFF4025 - Mercuric transport protein, MerT, at 232,696 to 233,037
GFF4025
GFF4026 - Periplasmic mercury(+2) binding protein, MerP, at 233,048 to 233,326
GFF4026
GFF4027 - hypothetical protein, at 233,323 to 233,553
GFF4027
GFF4028 - Cytochrome c, class I, at 233,719 to 235,668
GFF4028
GFF4029 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 235,798 to 238,728
GFF4029
Position (kb) 233
234
235
236 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 233.242 kb on + strand, within GFF4026 at 233.425 kb on + strand, within GFF4027 at 233.426 kb on - strand, within GFF4027 at 233.426 kb on - strand, within GFF4027 at 233.426 kb on - strand, within GFF4027 at 233.568 kb on + strand at 233.568 kb on + strand at 233.715 kb on + strand at 233.715 kb on + strand at 233.716 kb on - strand at 233.716 kb on - strand at 233.822 kb on + strand at 233.822 kb on + strand at 233.823 kb on - strand at 233.834 kb on - strand at 233.845 kb on + strand at 233.845 kb on + strand at 233.914 kb on + strand, within GFF4028 at 233.986 kb on - strand, within GFF4028 at 234.083 kb on + strand, within GFF4028 at 234.113 kb on + strand, within GFF4028 at 234.192 kb on - strand, within GFF4028 at 234.320 kb on + strand, within GFF4028 at 234.383 kb on + strand, within GFF4028 at 234.383 kb on + strand, within GFF4028 at 234.383 kb on + strand, within GFF4028 at 234.384 kb on - strand, within GFF4028 at 234.384 kb on - strand, within GFF4028 at 234.384 kb on - strand, within GFF4028 at 234.551 kb on + strand, within GFF4028 at 234.701 kb on + strand, within GFF4028 at 234.702 kb on - strand, within GFF4028 at 234.702 kb on - strand, within GFF4028 at 234.709 kb on + strand, within GFF4028 at 235.100 kb on + strand, within GFF4028 at 235.100 kb on + strand, within GFF4028 at 235.100 kb on + strand, within GFF4028 at 235.252 kb on + strand, within GFF4028 at 235.252 kb on + strand, within GFF4028 at 235.252 kb on + strand, within GFF4028 at 235.253 kb on - strand, within GFF4028 at 235.290 kb on - strand, within GFF4028 at 235.532 kb on + strand at 235.533 kb on - strand at 235.640 kb on + strand at 235.640 kb on + strand at 235.640 kb on + strand at 235.641 kb on - strand at 235.641 kb on - strand at 235.674 kb on - strand at 235.932 kb on + strand at 235.932 kb on + strand at 235.964 kb on - strand at 236.101 kb on + strand, within GFF4029 at 236.101 kb on + strand, within GFF4029 at 236.382 kb on + strand, within GFF4029 at 236.496 kb on - strand, within GFF4029 at 236.498 kb on + strand, within GFF4029 at 236.498 kb on + strand, within GFF4029 at 236.498 kb on + strand, within GFF4029 at 236.498 kb on + strand, within GFF4029 at 236.498 kb on + strand, within GFF4029 at 236.499 kb on - strand, within GFF4029 at 236.499 kb on - strand, within GFF4029 at 236.499 kb on - strand, within GFF4029 at 236.499 kb on - strand, within GFF4029
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 233,242 + GFF4026 0.70 +4.6 233,425 + GFF4027 0.44 +0.2 233,426 - GFF4027 0.45 -2.0 233,426 - GFF4027 0.45 -0.4 233,426 - GFF4027 0.45 -0.5 233,568 + -0.5 233,568 + +0.3 233,715 + -0.6 233,715 + +0.8 233,716 - -0.5 233,716 - -0.2 233,822 + -0.5 233,822 + +0.2 233,823 - -0.6 233,834 - +1.1 233,845 + -0.7 233,845 + +1.2 233,914 + GFF4028 0.10 +0.2 233,986 - GFF4028 0.14 -0.4 234,083 + GFF4028 0.19 -0.3 234,113 + GFF4028 0.20 +0.5 234,192 - GFF4028 0.24 -0.0 234,320 + GFF4028 0.31 -0.1 234,383 + GFF4028 0.34 -0.2 234,383 + GFF4028 0.34 -0.0 234,383 + GFF4028 0.34 -0.6 234,384 - GFF4028 0.34 +0.3 234,384 - GFF4028 0.34 -1.2 234,384 - GFF4028 0.34 +0.7 234,551 + GFF4028 0.43 +0.9 234,701 + GFF4028 0.50 -0.1 234,702 - GFF4028 0.50 +0.2 234,702 - GFF4028 0.50 -1.0 234,709 + GFF4028 0.51 +1.6 235,100 + GFF4028 0.71 -2.0 235,100 + GFF4028 0.71 -1.1 235,100 + GFF4028 0.71 -0.3 235,252 + GFF4028 0.79 -0.0 235,252 + GFF4028 0.79 -1.0 235,252 + GFF4028 0.79 +0.2 235,253 - GFF4028 0.79 -0.1 235,290 - GFF4028 0.81 -1.2 235,532 + -0.1 235,533 - -0.3 235,640 + -0.7 235,640 + -0.1 235,640 + +0.6 235,641 - +0.8 235,641 - -0.2 235,674 - -0.1 235,932 + +0.0 235,932 + -0.1 235,964 - +1.0 236,101 + GFF4029 0.10 +0.3 236,101 + GFF4029 0.10 -0.8 236,382 + GFF4029 0.20 -1.2 236,496 - GFF4029 0.24 -0.3 236,498 + GFF4029 0.24 -1.4 236,498 + GFF4029 0.24 -1.4 236,498 + GFF4029 0.24 -1.7 236,498 + GFF4029 0.24 -0.3 236,498 + GFF4029 0.24 -0.5 236,499 - GFF4029 0.24 -0.3 236,499 - GFF4029 0.24 -0.3 236,499 - GFF4029 0.24 +0.0 236,499 - GFF4029 0.24 +0.4
Or see this region's nucleotide sequence