Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3770 and GFF3771 are separated by 72 nucleotides GFF3771 and GFF3772 overlap by 4 nucleotides GFF3772 and GFF3773 are separated by 10 nucleotides
GFF3770 - hypothetical protein, at 261,504 to 262,868
GFF3770
GFF3771 - no description, at 262,941 to 263,495
GFF3771
GFF3772 - Arginase (EC 3.5.3.1), at 263,492 to 264,412
GFF3772
GFF3773 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 264,423 to 265,403
GFF3773
Position (kb)
262
263
264 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 262.041 kb on + strand, within GFF3770 at 262.041 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.092 kb on + strand, within GFF3770 at 262.093 kb on - strand, within GFF3770 at 262.093 kb on - strand, within GFF3770 at 262.093 kb on - strand, within GFF3770 at 262.093 kb on - strand, within GFF3770 at 262.137 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.347 kb on + strand, within GFF3770 at 262.348 kb on - strand, within GFF3770 at 262.348 kb on - strand, within GFF3770 at 262.348 kb on - strand, within GFF3770 at 262.348 kb on - strand, within GFF3770 at 262.503 kb on + strand, within GFF3770 at 262.504 kb on - strand, within GFF3770 at 262.504 kb on - strand, within GFF3770 at 262.866 kb on + strand at 262.899 kb on + strand at 262.899 kb on + strand at 262.900 kb on - strand at 262.900 kb on - strand at 262.900 kb on - strand at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.001 kb on + strand, within GFF3771 at 263.002 kb on - strand, within GFF3771 at 263.002 kb on - strand, within GFF3771 at 263.002 kb on - strand, within GFF3771 at 263.002 kb on - strand, within GFF3771 at 263.002 kb on - strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.079 kb on + strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.080 kb on - strand, within GFF3771 at 263.088 kb on + strand, within GFF3771 at 263.089 kb on - strand, within GFF3771 at 263.203 kb on - strand, within GFF3771 at 263.203 kb on - strand, within GFF3771 at 263.214 kb on + strand, within GFF3771 at 263.214 kb on + strand, within GFF3771 at 263.214 kb on + strand, within GFF3771 at 263.214 kb on + strand, within GFF3771 at 263.214 kb on + strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.215 kb on - strand, within GFF3771 at 263.445 kb on + strand at 263.445 kb on + strand at 263.446 kb on - strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.454 kb on + strand at 263.455 kb on - strand at 263.455 kb on - strand at 263.455 kb on - strand at 263.455 kb on - strand at 263.493 kb on + strand at 263.655 kb on + strand, within GFF3772 at 263.656 kb on - strand, within GFF3772 at 264.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 262,041 + GFF3770 0.39 +0.1 262,041 + GFF3770 0.39 -0.2 262,092 + GFF3770 0.43 +1.4 262,092 + GFF3770 0.43 +1.1 262,092 + GFF3770 0.43 +1.1 262,092 + GFF3770 0.43 -0.4 262,092 + GFF3770 0.43 -1.0 262,092 + GFF3770 0.43 +0.3 262,093 - GFF3770 0.43 +0.1 262,093 - GFF3770 0.43 -1.7 262,093 - GFF3770 0.43 -0.2 262,093 - GFF3770 0.43 -1.0 262,137 + GFF3770 0.46 -0.4 262,347 + GFF3770 0.62 +0.8 262,347 + GFF3770 0.62 +0.8 262,347 + GFF3770 0.62 -0.0 262,347 + GFF3770 0.62 -0.4 262,347 + GFF3770 0.62 +0.2 262,347 + GFF3770 0.62 -0.8 262,348 - GFF3770 0.62 -0.7 262,348 - GFF3770 0.62 +2.0 262,348 - GFF3770 0.62 +0.3 262,348 - GFF3770 0.62 -1.0 262,503 + GFF3770 0.73 -0.8 262,504 - GFF3770 0.73 -0.3 262,504 - GFF3770 0.73 +2.5 262,866 + -0.4 262,899 + -0.1 262,899 + +0.4 262,900 - -0.2 262,900 - -0.4 262,900 - +0.9 263,001 + GFF3771 0.11 -1.8 263,001 + GFF3771 0.11 -0.8 263,001 + GFF3771 0.11 +0.2 263,001 + GFF3771 0.11 -0.0 263,001 + GFF3771 0.11 -0.1 263,001 + GFF3771 0.11 +0.4 263,001 + GFF3771 0.11 +0.4 263,002 - GFF3771 0.11 +0.2 263,002 - GFF3771 0.11 -0.2 263,002 - GFF3771 0.11 -0.1 263,002 - GFF3771 0.11 -0.6 263,002 - GFF3771 0.11 +0.1 263,079 + GFF3771 0.25 +0.2 263,079 + GFF3771 0.25 -0.4 263,079 + GFF3771 0.25 +0.7 263,079 + GFF3771 0.25 +1.6 263,079 + GFF3771 0.25 -1.5 263,079 + GFF3771 0.25 -0.5 263,079 + GFF3771 0.25 +1.1 263,080 - GFF3771 0.25 -0.1 263,080 - GFF3771 0.25 -0.1 263,080 - GFF3771 0.25 -0.6 263,080 - GFF3771 0.25 -0.9 263,080 - GFF3771 0.25 +0.9 263,080 - GFF3771 0.25 -0.4 263,080 - GFF3771 0.25 -0.1 263,088 + GFF3771 0.26 -1.6 263,089 - GFF3771 0.27 +0.5 263,203 - GFF3771 0.47 -1.0 263,203 - GFF3771 0.47 -0.5 263,214 + GFF3771 0.49 +1.2 263,214 + GFF3771 0.49 +0.7 263,214 + GFF3771 0.49 -0.2 263,214 + GFF3771 0.49 +0.8 263,214 + GFF3771 0.49 -0.6 263,215 - GFF3771 0.49 +0.1 263,215 - GFF3771 0.49 +0.2 263,215 - GFF3771 0.49 +0.0 263,215 - GFF3771 0.49 -0.5 263,215 - GFF3771 0.49 +0.2 263,215 - GFF3771 0.49 -0.1 263,215 - GFF3771 0.49 -1.3 263,215 - GFF3771 0.49 +0.1 263,215 - GFF3771 0.49 -0.6 263,215 - GFF3771 0.49 -0.5 263,215 - GFF3771 0.49 -0.2 263,445 + +0.5 263,445 + -0.2 263,446 - +0.6 263,454 + +0.3 263,454 + -0.4 263,454 + +0.6 263,454 + +0.1 263,454 + -1.3 263,454 + -1.5 263,454 + +0.4 263,455 - -1.0 263,455 - -0.7 263,455 - +0.2 263,455 - -0.5 263,493 + -0.5 263,655 + GFF3772 0.18 -0.5 263,656 - GFF3772 0.18 +1.2 264,380 + -0.2
Or see this region's nucleotide sequence