Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3755 and GFF3756 are separated by 91 nucleotides GFF3756 and GFF3757 are separated by 96 nucleotides GFF3757 and GFF3758 are separated by 231 nucleotides
GFF3755 - Transcriptional regulator, GntR family, at 249,102 to 249,788
GFF3755
GFF3756 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 249,880 to 250,371
GFF3756
GFF3757 - hypothetical protein, at 250,468 to 250,890
GFF3757
GFF3758 - NAD-specific glutamate dehydrogenase (EC 1.4.1.2); NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 251,122 to 252,396
GFF3758
Position (kb) 250
251 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 249.847 kb on + strand at 249.862 kb on + strand at 249.862 kb on + strand at 249.863 kb on - strand at 249.881 kb on + strand at 249.881 kb on + strand at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.932 kb on + strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 249.933 kb on - strand, within GFF3756 at 250.028 kb on + strand, within GFF3756 at 250.029 kb on - strand, within GFF3756 at 250.029 kb on - strand, within GFF3756 at 250.029 kb on - strand, within GFF3756 at 250.029 kb on - strand, within GFF3756 at 250.410 kb on - strand at 250.411 kb on + strand at 250.412 kb on - strand at 250.412 kb on - strand at 250.418 kb on + strand at 250.418 kb on + strand at 250.418 kb on + strand at 250.418 kb on + strand at 250.418 kb on + strand at 250.419 kb on - strand at 250.419 kb on - strand at 250.419 kb on - strand at 250.419 kb on - strand at 250.454 kb on + strand at 250.454 kb on + strand at 250.455 kb on - strand at 250.526 kb on + strand, within GFF3757 at 250.526 kb on + strand, within GFF3757 at 250.526 kb on + strand, within GFF3757 at 250.526 kb on + strand, within GFF3757 at 250.548 kb on - strand, within GFF3757 at 250.691 kb on + strand, within GFF3757 at 250.691 kb on + strand, within GFF3757 at 250.691 kb on + strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.692 kb on - strand, within GFF3757 at 250.796 kb on + strand, within GFF3757 at 250.796 kb on + strand, within GFF3757 at 250.796 kb on + strand, within GFF3757 at 250.796 kb on + strand, within GFF3757 at 250.796 kb on + strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.797 kb on - strand, within GFF3757 at 250.894 kb on + strand at 250.894 kb on + strand at 250.894 kb on + strand at 250.894 kb on + strand at 250.941 kb on + strand at 250.941 kb on + strand at 250.941 kb on + strand at 250.942 kb on - strand at 250.942 kb on - strand at 250.942 kb on - strand at 250.970 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.992 kb on + strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 250.993 kb on - strand at 251.055 kb on + strand at 251.055 kb on + strand at 251.055 kb on + strand at 251.055 kb on + strand at 251.056 kb on - strand at 251.056 kb on - strand at 251.056 kb on - strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.085 kb on + strand at 251.086 kb on - strand at 251.086 kb on - strand at 251.086 kb on - strand at 251.086 kb on - strand at 251.086 kb on - strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.206 kb on + strand at 251.206 kb on + strand at 251.206 kb on + strand at 251.207 kb on - strand at 251.207 kb on - strand at 251.449 kb on + strand, within GFF3758 at 251.450 kb on - strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.527 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.626 kb on + strand, within GFF3758 at 251.627 kb on - strand, within GFF3758 at 251.627 kb on - strand, within GFF3758 at 251.627 kb on - strand, within GFF3758 at 251.627 kb on - strand, within GFF3758 at 251.627 kb on - strand, within GFF3758 at 251.627 kb on - strand, within GFF3758
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 249,847 + -0.5 249,862 + +0.8 249,862 + +0.1 249,863 - -0.7 249,881 + +0.2 249,881 + -0.5 249,932 + GFF3756 0.11 +0.2 249,932 + GFF3756 0.11 +0.2 249,932 + GFF3756 0.11 +0.3 249,932 + GFF3756 0.11 -0.5 249,932 + GFF3756 0.11 -0.4 249,932 + GFF3756 0.11 +0.2 249,932 + GFF3756 0.11 +1.1 249,932 + GFF3756 0.11 -0.4 249,932 + GFF3756 0.11 +0.4 249,932 + GFF3756 0.11 -0.5 249,932 + GFF3756 0.11 -0.8 249,933 - GFF3756 0.11 -0.7 249,933 - GFF3756 0.11 +0.2 249,933 - GFF3756 0.11 +0.6 249,933 - GFF3756 0.11 +0.1 249,933 - GFF3756 0.11 -0.7 249,933 - GFF3756 0.11 -0.5 249,933 - GFF3756 0.11 -0.1 249,933 - GFF3756 0.11 -1.5 249,933 - GFF3756 0.11 -1.3 249,933 - GFF3756 0.11 +0.3 249,933 - GFF3756 0.11 -0.1 249,933 - GFF3756 0.11 +1.2 250,028 + GFF3756 0.30 -0.9 250,029 - GFF3756 0.30 +0.2 250,029 - GFF3756 0.30 -0.2 250,029 - GFF3756 0.30 -0.5 250,029 - GFF3756 0.30 -1.3 250,410 - -0.6 250,411 + -0.1 250,412 - +0.2 250,412 - -0.1 250,418 + -1.6 250,418 + -0.2 250,418 + -0.0 250,418 + -0.8 250,418 + +2.2 250,419 - +0.4 250,419 - -0.2 250,419 - +0.2 250,419 - -0.2 250,454 + -0.2 250,454 + +1.7 250,455 - -0.2 250,526 + GFF3757 0.14 -0.5 250,526 + GFF3757 0.14 -0.6 250,526 + GFF3757 0.14 +0.7 250,526 + GFF3757 0.14 -1.1 250,548 - GFF3757 0.19 -1.3 250,691 + GFF3757 0.53 -0.7 250,691 + GFF3757 0.53 +0.2 250,691 + GFF3757 0.53 -0.6 250,692 - GFF3757 0.53 -0.5 250,692 - GFF3757 0.53 -0.0 250,692 - GFF3757 0.53 -1.9 250,692 - GFF3757 0.53 -1.2 250,692 - GFF3757 0.53 -0.8 250,692 - GFF3757 0.53 -0.5 250,692 - GFF3757 0.53 -0.0 250,692 - GFF3757 0.53 -2.2 250,692 - GFF3757 0.53 -1.0 250,796 + GFF3757 0.78 +0.0 250,796 + GFF3757 0.78 -0.5 250,796 + GFF3757 0.78 -0.4 250,796 + GFF3757 0.78 +0.8 250,796 + GFF3757 0.78 -1.3 250,797 - GFF3757 0.78 +1.5 250,797 - GFF3757 0.78 +0.3 250,797 - GFF3757 0.78 -0.1 250,797 - GFF3757 0.78 -0.2 250,797 - GFF3757 0.78 +1.0 250,797 - GFF3757 0.78 -0.9 250,797 - GFF3757 0.78 -1.0 250,894 + +0.6 250,894 + -0.1 250,894 + -0.5 250,894 + -0.5 250,941 + -0.6 250,941 + -0.4 250,941 + -0.3 250,942 - -1.0 250,942 - -0.0 250,942 - +0.5 250,970 + +0.3 250,992 + -1.8 250,992 + -1.8 250,992 + +1.8 250,992 + -1.2 250,992 + -1.0 250,992 + +1.2 250,992 + +1.7 250,992 + -1.9 250,992 + -0.6 250,992 + -0.8 250,992 + -0.7 250,992 + -0.4 250,992 + +0.2 250,992 + -2.0 250,992 + +0.4 250,992 + -1.0 250,993 - +0.0 250,993 - -1.0 250,993 - -1.8 250,993 - -0.0 250,993 - +0.7 250,993 - -0.8 250,993 - -0.3 250,993 - -0.2 251,055 + +0.2 251,055 + +0.5 251,055 + +0.1 251,055 + +0.8 251,056 - -1.2 251,056 - -0.1 251,056 - +0.5 251,085 + -1.0 251,085 + -0.3 251,085 + +0.4 251,085 + +2.8 251,085 + +0.3 251,085 + -0.1 251,085 + -0.4 251,085 + +0.3 251,086 - -0.0 251,086 - -0.2 251,086 - -2.2 251,086 - -0.2 251,086 - +0.2 251,176 + -1.5 251,176 + +0.5 251,176 + +1.8 251,176 + -1.2 251,176 + -0.6 251,176 + -2.0 251,176 + +2.8 251,176 + +1.8 251,176 + +0.6 251,177 - -0.8 251,177 - -0.7 251,177 - -0.7 251,177 - +0.4 251,177 - +0.6 251,177 - -1.3 251,177 - +0.3 251,177 - -0.8 251,177 - +0.1 251,177 - -0.9 251,177 - +0.2 251,177 - -1.0 251,206 + +0.2 251,206 + +0.4 251,206 + +1.3 251,207 - -0.0 251,207 - +0.2 251,449 + GFF3758 0.26 +0.5 251,450 - GFF3758 0.26 +0.5 251,527 + GFF3758 0.32 +1.0 251,527 + GFF3758 0.32 -0.0 251,527 + GFF3758 0.32 -0.8 251,527 + GFF3758 0.32 -0.1 251,527 + GFF3758 0.32 -1.7 251,527 + GFF3758 0.32 +1.4 251,527 + GFF3758 0.32 -0.6 251,527 + GFF3758 0.32 -0.1 251,626 + GFF3758 0.40 +1.0 251,626 + GFF3758 0.40 -0.7 251,626 + GFF3758 0.40 +1.8 251,626 + GFF3758 0.40 -2.0 251,626 + GFF3758 0.40 -0.1 251,626 + GFF3758 0.40 +0.2 251,627 - GFF3758 0.40 -0.4 251,627 - GFF3758 0.40 +1.2 251,627 - GFF3758 0.40 -0.5 251,627 - GFF3758 0.40 -1.9 251,627 - GFF3758 0.40 +0.8 251,627 - GFF3758 0.40 -0.4
Or see this region's nucleotide sequence