Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3656 and GFF3657 are separated by 124 nucleotides GFF3657 and GFF3658 overlap by 7 nucleotides GFF3658 and GFF3659 overlap by 1 nucleotides GFF3659 and GFF3660 overlap by 4 nucleotides
GFF3656 - hypothetical protein, at 159,545 to 160,009
GFF3656
GFF3657 - Mlr5572 protein, at 160,134 to 160,526
GFF3657
GFF3658 - hypothetical protein, at 160,520 to 160,906
GFF3658
GFF3659 - Two-component transcriptional response regulator, OmpR family, at 160,906 to 161,649
GFF3659
GFF3660 - integral membrane sensor signal transduction histidine kinase, at 161,646 to 163,073
GFF3660
Position (kb)
160
161
162 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.926 kb on + strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 159.927 kb on - strand, within GFF3656 at 160.008 kb on - strand at 160.008 kb on - strand at 160.074 kb on + strand at 160.075 kb on - strand at 160.075 kb on - strand at 160.075 kb on - strand at 160.222 kb on - strand, within GFF3657 at 160.222 kb on - strand, within GFF3657 at 160.230 kb on + strand, within GFF3657 at 160.230 kb on + strand, within GFF3657 at 160.293 kb on + strand, within GFF3657 at 160.326 kb on + strand, within GFF3657 at 160.326 kb on + strand, within GFF3657 at 160.326 kb on + strand, within GFF3657 at 160.326 kb on + strand, within GFF3657 at 160.326 kb on + strand, within GFF3657 at 160.327 kb on - strand, within GFF3657 at 160.327 kb on - strand, within GFF3657 at 160.327 kb on - strand, within GFF3657 at 160.327 kb on - strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.344 kb on + strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.345 kb on - strand, within GFF3657 at 160.467 kb on + strand, within GFF3657 at 160.467 kb on + strand, within GFF3657 at 160.467 kb on + strand, within GFF3657 at 160.467 kb on + strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.468 kb on - strand, within GFF3657 at 160.625 kb on + strand, within GFF3658 at 160.626 kb on - strand, within GFF3658 at 160.626 kb on - strand, within GFF3658 at 160.626 kb on - strand, within GFF3658 at 160.838 kb on + strand, within GFF3658 at 160.838 kb on + strand, within GFF3658 at 160.839 kb on - strand, within GFF3658 at 160.839 kb on - strand, within GFF3658 at 160.839 kb on - strand, within GFF3658 at 160.839 kb on - strand, within GFF3658 at 161.014 kb on + strand, within GFF3659 at 161.014 kb on + strand, within GFF3659 at 161.014 kb on + strand, within GFF3659 at 161.014 kb on + strand, within GFF3659 at 161.014 kb on + strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.015 kb on - strand, within GFF3659 at 161.092 kb on + strand, within GFF3659 at 161.093 kb on - strand, within GFF3659 at 161.093 kb on - strand, within GFF3659 at 161.248 kb on + strand, within GFF3659 at 161.248 kb on + strand, within GFF3659 at 161.248 kb on + strand, within GFF3659 at 161.248 kb on + strand, within GFF3659 at 161.248 kb on + strand, within GFF3659 at 161.249 kb on - strand, within GFF3659 at 161.249 kb on - strand, within GFF3659 at 161.249 kb on - strand, within GFF3659 at 161.527 kb on + strand, within GFF3659 at 161.527 kb on + strand, within GFF3659 at 161.527 kb on + strand, within GFF3659 at 161.528 kb on - strand, within GFF3659 at 161.528 kb on - strand, within GFF3659 at 161.614 kb on + strand at 161.615 kb on - strand at 161.913 kb on + strand, within GFF3660 at 161.914 kb on - strand, within GFF3660 at 161.914 kb on - strand, within GFF3660 at 161.914 kb on - strand, within GFF3660 at 161.914 kb on - strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.249 kb on + strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.250 kb on - strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.255 kb on + strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660 at 162.256 kb on - strand, within GFF3660
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 159,926 + GFF3656 0.82 -0.6 159,926 + GFF3656 0.82 +0.4 159,926 + GFF3656 0.82 +0.2 159,926 + GFF3656 0.82 -1.2 159,926 + GFF3656 0.82 -0.1 159,926 + GFF3656 0.82 -0.8 159,926 + GFF3656 0.82 -0.7 159,926 + GFF3656 0.82 -0.1 159,926 + GFF3656 0.82 +3.2 159,926 + GFF3656 0.82 -0.3 159,926 + GFF3656 0.82 -0.2 159,926 + GFF3656 0.82 -0.1 159,927 - GFF3656 0.82 +0.7 159,927 - GFF3656 0.82 +0.4 159,927 - GFF3656 0.82 -0.2 159,927 - GFF3656 0.82 -0.5 159,927 - GFF3656 0.82 -0.1 159,927 - GFF3656 0.82 -0.3 159,927 - GFF3656 0.82 +0.0 159,927 - GFF3656 0.82 +0.0 159,927 - GFF3656 0.82 +0.9 159,927 - GFF3656 0.82 -0.7 159,927 - GFF3656 0.82 -0.3 160,008 - +1.8 160,008 - -0.2 160,074 + +0.9 160,075 - +1.0 160,075 - -0.7 160,075 - -0.7 160,222 - GFF3657 0.22 -0.1 160,222 - GFF3657 0.22 +0.1 160,230 + GFF3657 0.24 +1.1 160,230 + GFF3657 0.24 -0.6 160,293 + GFF3657 0.40 +0.1 160,326 + GFF3657 0.49 -0.4 160,326 + GFF3657 0.49 -0.8 160,326 + GFF3657 0.49 -1.2 160,326 + GFF3657 0.49 +0.4 160,326 + GFF3657 0.49 -1.3 160,327 - GFF3657 0.49 -0.8 160,327 - GFF3657 0.49 -0.8 160,327 - GFF3657 0.49 +0.0 160,327 - GFF3657 0.49 -0.0 160,344 + GFF3657 0.53 -0.4 160,344 + GFF3657 0.53 -1.0 160,344 + GFF3657 0.53 -0.8 160,344 + GFF3657 0.53 -0.3 160,344 + GFF3657 0.53 +1.8 160,344 + GFF3657 0.53 +1.8 160,345 - GFF3657 0.54 +0.4 160,345 - GFF3657 0.54 +1.0 160,345 - GFF3657 0.54 -0.4 160,345 - GFF3657 0.54 -2.2 160,345 - GFF3657 0.54 -0.6 160,345 - GFF3657 0.54 -0.3 160,345 - GFF3657 0.54 -0.0 160,345 - GFF3657 0.54 +0.4 160,467 + GFF3657 0.85 -0.7 160,467 + GFF3657 0.85 +2.4 160,467 + GFF3657 0.85 -0.3 160,467 + GFF3657 0.85 -0.1 160,468 - GFF3657 0.85 -0.5 160,468 - GFF3657 0.85 -0.9 160,468 - GFF3657 0.85 +0.8 160,468 - GFF3657 0.85 +0.7 160,468 - GFF3657 0.85 +1.4 160,468 - GFF3657 0.85 +0.9 160,468 - GFF3657 0.85 +0.8 160,625 + GFF3658 0.27 -0.3 160,626 - GFF3658 0.27 +1.4 160,626 - GFF3658 0.27 +0.2 160,626 - GFF3658 0.27 -0.3 160,838 + GFF3658 0.82 -0.1 160,838 + GFF3658 0.82 +0.5 160,839 - GFF3658 0.82 -0.7 160,839 - GFF3658 0.82 +0.0 160,839 - GFF3658 0.82 -1.2 160,839 - GFF3658 0.82 +0.8 161,014 + GFF3659 0.15 +0.0 161,014 + GFF3659 0.15 -0.7 161,014 + GFF3659 0.15 +0.5 161,014 + GFF3659 0.15 -0.3 161,014 + GFF3659 0.15 -0.2 161,015 - GFF3659 0.15 -1.5 161,015 - GFF3659 0.15 -1.2 161,015 - GFF3659 0.15 +0.2 161,015 - GFF3659 0.15 +1.0 161,015 - GFF3659 0.15 -2.0 161,015 - GFF3659 0.15 +1.0 161,015 - GFF3659 0.15 -0.2 161,015 - GFF3659 0.15 +0.3 161,015 - GFF3659 0.15 +0.5 161,092 + GFF3659 0.25 -0.2 161,093 - GFF3659 0.25 +0.2 161,093 - GFF3659 0.25 +0.0 161,248 + GFF3659 0.46 -1.4 161,248 + GFF3659 0.46 +0.1 161,248 + GFF3659 0.46 +0.0 161,248 + GFF3659 0.46 -0.8 161,248 + GFF3659 0.46 -0.3 161,249 - GFF3659 0.46 +0.0 161,249 - GFF3659 0.46 +1.2 161,249 - GFF3659 0.46 -0.0 161,527 + GFF3659 0.83 -0.4 161,527 + GFF3659 0.83 +0.8 161,527 + GFF3659 0.83 +1.8 161,528 - GFF3659 0.84 -0.2 161,528 - GFF3659 0.84 -0.3 161,614 + +0.2 161,615 - -0.8 161,913 + GFF3660 0.19 -0.8 161,914 - GFF3660 0.19 -0.8 161,914 - GFF3660 0.19 -0.8 161,914 - GFF3660 0.19 -1.2 161,914 - GFF3660 0.19 -2.3 162,249 + GFF3660 0.42 -1.0 162,249 + GFF3660 0.42 -1.3 162,249 + GFF3660 0.42 +0.0 162,249 + GFF3660 0.42 -0.8 162,249 + GFF3660 0.42 -1.9 162,249 + GFF3660 0.42 -2.4 162,249 + GFF3660 0.42 -0.1 162,250 - GFF3660 0.42 +1.4 162,250 - GFF3660 0.42 -2.9 162,250 - GFF3660 0.42 +1.5 162,250 - GFF3660 0.42 +0.3 162,250 - GFF3660 0.42 +0.2 162,250 - GFF3660 0.42 -1.8 162,250 - GFF3660 0.42 -0.3 162,250 - GFF3660 0.42 -1.6 162,255 + GFF3660 0.43 -1.6 162,255 + GFF3660 0.43 -0.5 162,255 + GFF3660 0.43 +0.2 162,255 + GFF3660 0.43 -0.8 162,255 + GFF3660 0.43 -0.6 162,255 + GFF3660 0.43 -0.4 162,255 + GFF3660 0.43 -1.6 162,255 + GFF3660 0.43 -0.4 162,255 + GFF3660 0.43 -0.3 162,255 + GFF3660 0.43 -1.2 162,255 + GFF3660 0.43 +0.2 162,255 + GFF3660 0.43 -0.8 162,255 + GFF3660 0.43 +0.2 162,256 - GFF3660 0.43 -1.5 162,256 - GFF3660 0.43 -0.1 162,256 - GFF3660 0.43 -1.3 162,256 - GFF3660 0.43 +0.1 162,256 - GFF3660 0.43 -2.7 162,256 - GFF3660 0.43 -0.6 162,256 - GFF3660 0.43 -1.1 162,256 - GFF3660 0.43 +0.4
Or see this region's nucleotide sequence