Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF356 and GFF357 are separated by 73 nucleotides GFF357 and GFF358 are separated by 137 nucleotides GFF358 and GFF359 are separated by 91 nucleotides
GFF356 - L-asparaginase (EC 3.5.1.1), at 386,853 to 387,815
GFF356
GFF357 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 387,889 to 388,572
GFF357
GFF358 - hypothetical protein, at 388,710 to 389,075
GFF358
GFF359 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 389,167 to 390,174
GFF359
Position (kb) 388
389
390 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 387.715 kb on + strand, within GFF356 at 387.716 kb on - strand, within GFF356 at 387.716 kb on - strand, within GFF356 at 387.847 kb on + strand at 387.847 kb on + strand at 387.848 kb on - strand at 387.853 kb on + strand at 387.853 kb on + strand at 387.853 kb on + strand at 387.853 kb on + strand at 387.854 kb on - strand at 387.861 kb on - strand at 387.861 kb on - strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.888 kb on + strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.889 kb on - strand at 387.909 kb on + strand at 388.037 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.297 kb on + strand, within GFF357 at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.611 kb on + strand at 388.626 kb on + strand at 388.626 kb on + strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.692 kb on + strand at 388.693 kb on - strand at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.240 kb on - strand at 389.240 kb on - strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.650 kb on + strand, within GFF359 at 389.681 kb on - strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 387,715 + GFF356 0.90 -0.9 387,716 - GFF356 0.90 -0.2 387,716 - GFF356 0.90 -1.9 387,847 + +0.0 387,847 + -0.2 387,848 - -1.5 387,853 + -1.4 387,853 + +1.0 387,853 + +1.1 387,853 + -0.3 387,854 - -1.6 387,861 - +0.3 387,861 - +0.1 387,888 + -0.8 387,888 + +0.3 387,888 + -0.3 387,888 + -0.5 387,888 + +1.1 387,888 + -0.3 387,888 + +1.4 387,888 + -0.0 387,888 + +0.9 387,888 + -0.5 387,888 + -0.6 387,888 + -2.3 387,888 + -2.2 387,888 + -0.8 387,888 + +0.2 387,888 + +0.8 387,888 + -0.4 387,888 + +0.1 387,888 + +0.4 387,888 + -0.2 387,888 + -0.2 387,888 + -0.2 387,888 + -0.0 387,888 + -0.0 387,888 + -0.4 387,888 + +0.1 387,888 + -0.4 387,888 + -0.7 387,888 + -0.3 387,888 + -0.2 387,888 + -0.1 387,889 - -1.3 387,889 - -0.0 387,889 - -1.5 387,889 - -0.1 387,889 - -0.2 387,889 - -0.1 387,889 - -1.5 387,889 - -0.1 387,889 - -0.4 387,889 - -0.6 387,889 - +0.8 387,889 - +0.1 387,889 - +0.4 387,889 - -0.3 387,889 - +1.6 387,889 - +1.9 387,909 + -0.5 388,037 + GFF357 0.22 -0.6 388,258 + GFF357 0.54 -0.7 388,258 + GFF357 0.54 -0.7 388,258 + GFF357 0.54 +0.0 388,258 + GFF357 0.54 -0.4 388,258 + GFF357 0.54 +1.6 388,258 + GFF357 0.54 -0.2 388,258 + GFF357 0.54 -0.7 388,258 + GFF357 0.54 +0.9 388,258 + GFF357 0.54 -0.0 388,258 + GFF357 0.54 -0.7 388,258 + GFF357 0.54 -2.8 388,258 + GFF357 0.54 -1.0 388,259 - GFF357 0.54 +0.5 388,259 - GFF357 0.54 -0.1 388,259 - GFF357 0.54 -0.3 388,259 - GFF357 0.54 +1.7 388,259 - GFF357 0.54 +0.7 388,259 - GFF357 0.54 -0.5 388,259 - GFF357 0.54 -0.6 388,259 - GFF357 0.54 -0.2 388,259 - GFF357 0.54 -0.5 388,259 - GFF357 0.54 +0.1 388,259 - GFF357 0.54 -0.0 388,259 - GFF357 0.54 +0.8 388,259 - GFF357 0.54 +0.5 388,259 - GFF357 0.54 -0.2 388,259 - GFF357 0.54 -1.0 388,259 - GFF357 0.54 -0.5 388,259 - GFF357 0.54 -0.2 388,259 - GFF357 0.54 +0.1 388,259 - GFF357 0.54 +0.6 388,259 - GFF357 0.54 -0.1 388,259 - GFF357 0.54 +0.8 388,259 - GFF357 0.54 +0.4 388,259 - GFF357 0.54 -0.1 388,261 + GFF357 0.54 -0.3 388,261 + GFF357 0.54 +1.3 388,261 + GFF357 0.54 -1.0 388,261 + GFF357 0.54 +0.5 388,261 + GFF357 0.54 +0.3 388,261 + GFF357 0.54 -0.7 388,261 + GFF357 0.54 -0.4 388,261 + GFF357 0.54 +1.0 388,262 - GFF357 0.55 +0.7 388,262 - GFF357 0.55 -0.5 388,262 - GFF357 0.55 +0.6 388,262 - GFF357 0.55 +0.7 388,262 - GFF357 0.55 +0.1 388,262 - GFF357 0.55 -0.2 388,262 - GFF357 0.55 -0.7 388,262 - GFF357 0.55 -1.0 388,297 + GFF357 0.60 +0.3 388,570 + -0.4 388,570 + +0.7 388,570 + +3.3 388,570 + -1.5 388,570 + -0.7 388,570 + +0.1 388,570 + -0.3 388,570 + +0.1 388,570 + +0.0 388,570 + +0.3 388,570 + +0.0 388,570 + -0.3 388,570 + -0.3 388,570 + -1.8 388,570 + -0.1 388,570 + -0.3 388,571 - -0.1 388,571 - -0.9 388,571 - +0.0 388,571 - -1.7 388,571 - +0.3 388,571 - +0.5 388,571 - +1.9 388,571 - +1.0 388,571 - -0.4 388,571 - +0.2 388,571 - +1.1 388,571 - -0.2 388,571 - -0.0 388,611 + +0.5 388,626 + -1.3 388,626 + -0.4 388,627 - +0.1 388,627 - +0.8 388,627 - -0.0 388,627 - -1.5 388,692 + -1.1 388,693 - -1.5 388,842 + GFF358 0.36 -1.2 388,842 + GFF358 0.36 +1.4 388,842 + GFF358 0.36 -0.8 388,842 + GFF358 0.36 +0.3 388,842 + GFF358 0.36 +0.3 388,842 + GFF358 0.36 -0.0 388,842 + GFF358 0.36 -1.3 388,842 + GFF358 0.36 -1.9 388,842 + GFF358 0.36 -0.1 388,843 - GFF358 0.36 -0.2 388,843 - GFF358 0.36 -0.7 388,843 - GFF358 0.36 -1.0 388,843 - GFF358 0.36 -0.5 388,843 - GFF358 0.36 -1.5 389,172 + -0.5 389,172 + -0.4 389,172 + +0.3 389,172 + -0.1 389,172 + -0.3 389,172 + -0.7 389,173 - +1.3 389,173 - -0.3 389,173 - -1.1 389,173 - -0.8 389,173 - +0.4 389,173 - -0.9 389,173 - -0.7 389,173 - -0.6 389,173 - +0.1 389,191 + +0.2 389,191 + -0.1 389,191 + +0.7 389,192 - -0.6 389,192 - -1.3 389,192 - -1.1 389,239 + -1.2 389,239 + -0.3 389,239 + -2.5 389,240 - -0.3 389,240 - -0.8 389,254 + -1.9 389,254 + -1.0 389,254 + -0.2 389,254 + +0.3 389,254 + +0.9 389,254 + +1.3 389,254 + -0.2 389,254 + -0.0 389,255 - -0.3 389,255 - -0.0 389,255 - +0.7 389,255 - -1.9 389,255 - +0.0 389,255 - +1.0 389,255 - -0.1 389,255 - -0.3 389,256 + +0.3 389,256 + -0.2 389,256 + +0.0 389,256 + -0.3 389,256 + -0.4 389,256 + -0.6 389,256 + -0.9 389,256 + +1.6 389,257 - -0.9 389,257 - -0.2 389,257 - -0.0 389,257 - +0.1 389,257 - +0.5 389,257 - +0.4 389,257 - -0.1 389,257 - +1.0 389,257 - -1.4 389,257 - -1.4 389,533 + GFF359 0.36 +0.4 389,533 + GFF359 0.36 -0.7 389,533 + GFF359 0.36 -1.0 389,533 + GFF359 0.36 +0.3 389,533 + GFF359 0.36 -0.6 389,533 + GFF359 0.36 -0.3 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 -2.4 389,533 + GFF359 0.36 -0.2 389,533 + GFF359 0.36 -0.3 389,533 + GFF359 0.36 -0.7 389,533 + GFF359 0.36 -0.0 389,534 - GFF359 0.36 -0.4 389,534 - GFF359 0.36 -0.2 389,534 - GFF359 0.36 -0.7 389,534 - GFF359 0.36 -1.2 389,534 - GFF359 0.36 -0.3 389,534 - GFF359 0.36 -1.0 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 +0.1 389,534 - GFF359 0.36 -0.2 389,534 - GFF359 0.36 -1.7 389,534 - GFF359 0.36 -0.0 389,650 + GFF359 0.48 -0.4 389,681 - GFF359 0.51 -0.4 390,055 + GFF359 0.88 -0.5 390,055 + GFF359 0.88 -0.7 390,055 + GFF359 0.88 -0.7 390,056 - GFF359 0.88 -0.9 390,056 - GFF359 0.88 -1.0 390,056 - GFF359 0.88 +1.0 390,056 - GFF359 0.88 +0.8 390,073 + GFF359 0.90 -0.7 390,073 + GFF359 0.90 +0.1 390,074 - +1.9 390,074 - -0.4 390,074 - -0.6 390,074 - -0.3
Or see this region's nucleotide sequence