Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3377 and GFF3378 are separated by 79 nucleotides GFF3378 and GFF3379 are separated by 6 nucleotides GFF3379 and GFF3380 are separated by 126 nucleotides
GFF3377 - Enoyl-CoA hydratase (EC 4.2.1.17) @ Enoyl-CoA hydratase EchA5 (EC 4.2.1.17), at 197,402 to 198,184
GFF3377
GFF3378 - hypothetical protein, at 198,264 to 199,076
GFF3378
GFF3379 - Aromatic--CoA ligase, at 199,083 to 200,894
GFF3379
GFF3380 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 201,021 to 201,968
GFF3380
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 198.172 kb on - strand at 198.172 kb on - strand at 198.172 kb on - strand at 198.238 kb on - strand at 198.256 kb on - strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.274 kb on + strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.275 kb on - strand at 198.278 kb on + strand at 198.278 kb on + strand at 198.278 kb on + strand at 198.279 kb on - strand at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.468 kb on + strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 198.469 kb on - strand, within GFF3378 at 199.183 kb on + strand at 199.183 kb on + strand at 199.183 kb on + strand at 199.184 kb on - strand at 199.184 kb on - strand at 199.184 kb on - strand at 199.184 kb on - strand at 199.184 kb on - strand at 199.567 kb on + strand, within GFF3379 at 199.570 kb on + strand, within GFF3379 at 199.571 kb on - strand, within GFF3379 at 199.874 kb on - strand, within GFF3379 at 199.933 kb on + strand, within GFF3379 at 200.050 kb on + strand, within GFF3379 at 200.050 kb on + strand, within GFF3379 at 200.051 kb on - strand, within GFF3379 at 200.255 kb on - strand, within GFF3379 at 200.420 kb on - strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.440 kb on + strand, within GFF3379 at 200.441 kb on - strand, within GFF3379 at 200.677 kb on + strand, within GFF3379 at 200.677 kb on + strand, within GFF3379 at 200.785 kb on + strand at 200.785 kb on + strand at 200.785 kb on + strand at 200.786 kb on - strand at 200.844 kb on + strand at 200.942 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.063 kb on + strand at 201.454 kb on + strand, within GFF3380 at 201.454 kb on + strand, within GFF3380 at 201.686 kb on + strand, within GFF3380
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 198,172 - +0.5 198,172 - -0.1 198,172 - +0.1 198,238 - -0.4 198,256 - +0.7 198,274 + -0.4 198,274 + +0.0 198,274 + -1.5 198,274 + -1.5 198,275 - -0.8 198,275 - -0.4 198,275 - -0.6 198,275 - +0.5 198,278 + -0.4 198,278 + -0.5 198,278 + +0.3 198,279 - -0.5 198,468 + GFF3378 0.25 -0.1 198,468 + GFF3378 0.25 +0.8 198,468 + GFF3378 0.25 +0.3 198,468 + GFF3378 0.25 +0.4 198,468 + GFF3378 0.25 -0.2 198,468 + GFF3378 0.25 +0.8 198,468 + GFF3378 0.25 +0.0 198,468 + GFF3378 0.25 +0.2 198,469 - GFF3378 0.25 -0.1 198,469 - GFF3378 0.25 +0.1 198,469 - GFF3378 0.25 +0.3 198,469 - GFF3378 0.25 -0.0 198,469 - GFF3378 0.25 -0.5 198,469 - GFF3378 0.25 +0.2 198,469 - GFF3378 0.25 +0.4 198,469 - GFF3378 0.25 -0.1 198,469 - GFF3378 0.25 +0.9 199,183 + -0.2 199,183 + +0.7 199,183 + -0.6 199,184 - +0.0 199,184 - +0.6 199,184 - +0.1 199,184 - -0.0 199,184 - -3.4 199,567 + GFF3379 0.27 -0.5 199,570 + GFF3379 0.27 +1.4 199,571 - GFF3379 0.27 -0.9 199,874 - GFF3379 0.44 -0.6 199,933 + GFF3379 0.47 -0.5 200,050 + GFF3379 0.53 -0.1 200,050 + GFF3379 0.53 -0.9 200,051 - GFF3379 0.53 -0.1 200,255 - GFF3379 0.65 -0.4 200,420 - GFF3379 0.74 +1.8 200,440 + GFF3379 0.75 +0.2 200,440 + GFF3379 0.75 -0.2 200,440 + GFF3379 0.75 +0.1 200,441 - GFF3379 0.75 -1.8 200,677 + GFF3379 0.88 +0.6 200,677 + GFF3379 0.88 +0.8 200,785 + -0.5 200,785 + -2.6 200,785 + -0.2 200,786 - -0.5 200,844 + +0.0 200,942 - -1.6 201,023 - -0.0 201,023 - -0.7 201,023 - +0.6 201,023 - -2.1 201,063 + -0.1 201,454 + GFF3380 0.46 +0.2 201,454 + GFF3380 0.46 -1.0 201,686 + GFF3380 0.70 -1.4
Or see this region's nucleotide sequence