Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3346 and GFF3347 are separated by 121 nucleotides GFF3347 and GFF3348 are separated by 4 nucleotides GFF3348 and GFF3349 are separated by 160 nucleotides
GFF3346 - hypothetical protein, at 169,552 to 169,914
GFF3346
GFF3347 - Adenylate cyclase (EC 4.6.1.1), at 170,036 to 170,557
GFF3347
GFF3348 - Putative lipase, at 170,562 to 171,203
GFF3348
GFF3349 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 171,364 to 172,545
GFF3349
Position (kb) 170
171
172 Strain fitness (log2 ratio) -1
0
1 at 169.913 kb on - strand at 169.924 kb on - strand at 169.924 kb on - strand at 170.125 kb on - strand, within GFF3347 at 170.223 kb on - strand, within GFF3347 at 170.261 kb on + strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.528 kb on + strand at 170.529 kb on - strand at 170.618 kb on - strand at 170.680 kb on + strand, within GFF3348 at 170.680 kb on + strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.728 kb on + strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.917 kb on + strand, within GFF3348 at 170.918 kb on - strand, within GFF3348 at 171.217 kb on + strand at 171.218 kb on - strand at 171.269 kb on + strand at 171.269 kb on + strand at 171.410 kb on + strand at 171.518 kb on - strand, within GFF3349 at 171.518 kb on - strand, within GFF3349 at 171.601 kb on + strand, within GFF3349 at 171.601 kb on + strand, within GFF3349 at 171.733 kb on + strand, within GFF3349 at 171.766 kb on - strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.803 kb on - strand, within GFF3349 at 171.803 kb on - strand, within GFF3349 at 171.899 kb on - strand, within GFF3349 at 171.988 kb on + strand, within GFF3349 at 172.069 kb on + strand, within GFF3349 at 172.070 kb on - strand, within GFF3349 at 172.070 kb on - strand, within GFF3349 at 172.150 kb on + strand, within GFF3349 at 172.151 kb on - strand, within GFF3349 at 172.151 kb on - strand, within GFF3349
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 169,913 - -1.2 169,924 - +0.9 169,924 - -0.2 170,125 - GFF3347 0.17 +0.3 170,223 - GFF3347 0.36 +0.4 170,261 + GFF3347 0.43 +1.4 170,262 - GFF3347 0.43 -0.0 170,262 - GFF3347 0.43 +0.2 170,528 + +0.2 170,529 - +0.7 170,618 - -0.4 170,680 + GFF3348 0.18 -0.3 170,680 + GFF3348 0.18 +0.4 170,681 - GFF3348 0.19 -0.9 170,681 - GFF3348 0.19 -0.8 170,728 + GFF3348 0.26 -0.9 170,729 - GFF3348 0.26 +0.3 170,729 - GFF3348 0.26 -0.1 170,729 - GFF3348 0.26 -0.6 170,729 - GFF3348 0.26 -0.6 170,729 - GFF3348 0.26 -0.2 170,917 + GFF3348 0.55 -0.7 170,918 - GFF3348 0.55 -1.1 171,217 + +0.6 171,218 - -0.4 171,269 + +1.1 171,269 + +0.4 171,410 + +0.4 171,518 - GFF3349 0.13 +1.1 171,518 - GFF3349 0.13 -0.1 171,601 + GFF3349 0.20 -0.7 171,601 + GFF3349 0.20 +0.2 171,733 + GFF3349 0.31 -0.9 171,766 - GFF3349 0.34 -0.1 171,802 + GFF3349 0.37 +0.3 171,802 + GFF3349 0.37 -0.8 171,802 + GFF3349 0.37 +0.1 171,803 - GFF3349 0.37 +1.4 171,803 - GFF3349 0.37 -0.3 171,899 - GFF3349 0.45 -0.1 171,988 + GFF3349 0.53 +0.0 172,069 + GFF3349 0.60 -0.4 172,070 - GFF3349 0.60 -1.7 172,070 - GFF3349 0.60 +0.1 172,150 + GFF3349 0.66 +0.6 172,151 - GFF3349 0.67 -1.1 172,151 - GFF3349 0.67 -0.7
Or see this region's nucleotide sequence