Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3329 and GFF3330 overlap by 4 nucleotides GFF3330 and GFF3331 are separated by 158 nucleotides GFF3331 and GFF3332 are separated by 184 nucleotides
GFF3329 - Terpene utilization protein AtuA, at 155,050 to 156,858
GFF3329
GFF3330 - Isohexenylglutaconyl-CoA hydratase (EC 4.2.1.57), at 156,855 to 157,637
GFF3330
GFF3331 - Glutathione S-transferase (EC 2.5.1.18), at 157,796 to 158,404
GFF3331
GFF3332 - UPF0391 membrane protein RPC_2356, at 158,589 to 158,774
GFF3332
Position (kb)
156
157
158 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 155.903 kb on + strand, within GFF3329 at 155.903 kb on + strand, within GFF3329 at 155.904 kb on - strand, within GFF3329 at 155.904 kb on - strand, within GFF3329 at 155.921 kb on + strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 156.399 kb on - strand, within GFF3329 at 156.399 kb on - strand, within GFF3329 at 156.399 kb on - strand, within GFF3329 at 156.677 kb on + strand, within GFF3329 at 156.677 kb on + strand, within GFF3329 at 156.741 kb on - strand at 156.755 kb on + strand at 156.755 kb on + strand at 156.756 kb on - strand at 156.756 kb on - strand at 156.756 kb on - strand at 156.756 kb on - strand at 156.756 kb on - strand at 156.756 kb on - strand at 156.756 kb on - strand at 157.003 kb on + strand, within GFF3330 at 157.003 kb on + strand, within GFF3330 at 157.003 kb on + strand, within GFF3330 at 157.003 kb on + strand, within GFF3330 at 157.003 kb on + strand, within GFF3330 at 157.003 kb on + strand, within GFF3330 at 157.004 kb on - strand, within GFF3330 at 157.004 kb on - strand, within GFF3330 at 157.004 kb on - strand, within GFF3330 at 157.004 kb on - strand, within GFF3330 at 157.382 kb on - strand, within GFF3330 at 157.393 kb on + strand, within GFF3330 at 157.393 kb on + strand, within GFF3330 at 157.797 kb on + strand at 157.798 kb on - strand at 157.798 kb on - strand at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.971 kb on + strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 157.972 kb on - strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.190 kb on + strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.191 kb on - strand, within GFF3331 at 158.254 kb on - strand, within GFF3331 at 158.254 kb on - strand, within GFF3331 at 158.254 kb on - strand, within GFF3331 at 158.254 kb on - strand, within GFF3331 at 158.274 kb on + strand, within GFF3331 at 158.274 kb on + strand, within GFF3331 at 158.274 kb on + strand, within GFF3331 at 158.275 kb on - strand, within GFF3331 at 158.275 kb on - strand, within GFF3331 at 158.275 kb on - strand, within GFF3331 at 158.278 kb on - strand, within GFF3331 at 158.391 kb on + strand at 158.391 kb on + strand at 158.392 kb on - strand at 158.392 kb on - strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.394 kb on + strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.395 kb on - strand at 158.412 kb on - strand at 158.412 kb on - strand at 158.412 kb on - strand at 158.464 kb on + strand at 158.464 kb on + strand at 158.496 kb on - strand at 158.496 kb on - strand at 158.543 kb on + strand at 158.543 kb on + strand at 158.543 kb on + strand at 158.543 kb on + strand at 158.544 kb on - strand at 158.544 kb on - strand at 158.544 kb on - strand at 158.544 kb on - strand at 158.598 kb on + strand at 158.598 kb on + strand at 158.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 155,903 + GFF3329 0.47 +1.4 155,903 + GFF3329 0.47 +0.3 155,904 - GFF3329 0.47 +0.0 155,904 - GFF3329 0.47 -1.6 155,921 + GFF3329 0.48 -1.0 155,922 - GFF3329 0.48 +0.0 155,922 - GFF3329 0.48 -0.4 155,922 - GFF3329 0.48 +0.7 155,922 - GFF3329 0.48 -0.7 155,922 - GFF3329 0.48 -1.1 155,922 - GFF3329 0.48 +0.4 155,922 - GFF3329 0.48 +0.3 156,399 - GFF3329 0.75 -0.5 156,399 - GFF3329 0.75 -0.5 156,399 - GFF3329 0.75 +0.0 156,677 + GFF3329 0.90 -0.6 156,677 + GFF3329 0.90 -0.6 156,741 - +0.5 156,755 + -0.9 156,755 + -0.8 156,756 - -0.7 156,756 - -0.2 156,756 - -0.3 156,756 - +0.4 156,756 - +1.1 156,756 - +0.2 156,756 - -0.7 157,003 + GFF3330 0.19 +0.1 157,003 + GFF3330 0.19 +0.8 157,003 + GFF3330 0.19 +0.4 157,003 + GFF3330 0.19 +0.0 157,003 + GFF3330 0.19 -0.3 157,003 + GFF3330 0.19 +0.4 157,004 - GFF3330 0.19 +0.2 157,004 - GFF3330 0.19 +2.2 157,004 - GFF3330 0.19 -0.5 157,004 - GFF3330 0.19 -0.3 157,382 - GFF3330 0.67 +0.3 157,393 + GFF3330 0.69 +0.2 157,393 + GFF3330 0.69 +0.2 157,797 + -0.1 157,798 - +0.1 157,798 - +1.4 157,971 + GFF3331 0.29 -0.4 157,971 + GFF3331 0.29 -0.7 157,971 + GFF3331 0.29 -0.4 157,971 + GFF3331 0.29 -0.7 157,971 + GFF3331 0.29 +0.2 157,971 + GFF3331 0.29 +1.0 157,971 + GFF3331 0.29 -0.1 157,971 + GFF3331 0.29 -0.4 157,972 - GFF3331 0.29 +0.1 157,972 - GFF3331 0.29 -1.8 157,972 - GFF3331 0.29 -0.1 157,972 - GFF3331 0.29 -1.5 157,972 - GFF3331 0.29 -0.3 157,972 - GFF3331 0.29 -0.1 158,190 + GFF3331 0.65 -0.3 158,190 + GFF3331 0.65 -0.1 158,190 + GFF3331 0.65 +0.4 158,190 + GFF3331 0.65 +0.4 158,190 + GFF3331 0.65 +0.8 158,190 + GFF3331 0.65 -2.0 158,190 + GFF3331 0.65 +0.1 158,191 - GFF3331 0.65 +0.5 158,191 - GFF3331 0.65 -0.0 158,191 - GFF3331 0.65 +0.4 158,191 - GFF3331 0.65 -0.1 158,191 - GFF3331 0.65 +1.2 158,191 - GFF3331 0.65 -0.1 158,191 - GFF3331 0.65 -0.8 158,254 - GFF3331 0.75 -1.4 158,254 - GFF3331 0.75 +0.2 158,254 - GFF3331 0.75 -0.8 158,254 - GFF3331 0.75 -0.2 158,274 + GFF3331 0.78 -1.4 158,274 + GFF3331 0.78 -0.1 158,274 + GFF3331 0.78 -0.5 158,275 - GFF3331 0.79 -0.3 158,275 - GFF3331 0.79 -0.9 158,275 - GFF3331 0.79 +1.9 158,278 - GFF3331 0.79 -0.6 158,391 + -0.6 158,391 + +0.1 158,392 - -0.8 158,392 - -1.8 158,394 + +0.9 158,394 + +1.9 158,394 + -2.0 158,394 + -0.0 158,394 + +0.2 158,394 + -0.4 158,394 + -0.8 158,394 + +0.2 158,394 + +0.3 158,394 + +0.8 158,394 + -1.2 158,395 - -1.9 158,395 - +0.3 158,395 - +0.0 158,395 - -0.4 158,395 - -0.6 158,395 - -0.1 158,395 - -0.4 158,395 - +0.1 158,395 - +0.2 158,395 - +0.2 158,395 - +0.1 158,395 - -0.4 158,412 - -0.0 158,412 - -0.8 158,412 - +0.1 158,464 + +0.9 158,464 + -0.2 158,496 - +0.1 158,496 - -0.4 158,543 + -0.6 158,543 + +0.1 158,543 + +0.0 158,543 + -0.4 158,544 - -0.1 158,544 - -1.6 158,544 - -1.4 158,544 - -0.3 158,598 + -0.6 158,598 + -1.2 158,599 - +0.8
Or see this region's nucleotide sequence