Strain Fitness in Variovorax sp. SCN45 around GFF3311

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
137,127	+		GFF3310	0.64	+0.9
137,127	+		GFF3310	0.64	+0.3
137,127	+		GFF3310	0.64	+0.1
137,128	-		GFF3310	0.64	-0.1
137,128	-		GFF3310	0.64	-0.3
137,187	+		GFF3310	0.66	-0.4
137,187	+		GFF3310	0.66	+1.2
137,187	+		GFF3310	0.66	-0.2
137,187	+		GFF3310	0.66	-0.2
137,187	+		GFF3310	0.66	-0.7
137,187	+		GFF3310	0.66	-0.4
137,188	-		GFF3310	0.66	+0.3
137,188	-		GFF3310	0.66	-0.1
137,188	-		GFF3310	0.66	+0.5
137,188	-		GFF3310	0.66	-0.8
137,188	-		GFF3310	0.66	+0.9
137,275	-		GFF3310	0.70	-1.4
137,275	-		GFF3310	0.70	-1.1
137,275	-		GFF3310	0.70	+0.1
137,424	+		GFF3310	0.76	-0.6
137,425	-		GFF3310	0.76	+1.2
137,583	+		GFF3310	0.83	+0.9
137,584	-		GFF3310	0.83	+0.7
137,611	-		GFF3310	0.84	-0.4
137,697	+		GFF3310	0.87	-0.5
137,698	-		GFF3310	0.87	+0.3
137,698	-		GFF3310	0.87	-0.5
137,769	+				-0.2
137,769	+				-0.2
137,769	+				-0.5
137,769	+				-0.1
137,770	-				+0.0
137,770	-				-1.1
137,770	-				-0.5
137,770	-				+0.3
137,770	-				-0.8
137,851	-				-0.7
137,851	-				-1.9
137,851	-				+0.8
137,851	-				-1.5
137,851	-				-0.8
137,851	-				-0.3
138,040	+				+1.1
138,040	+				-0.1
138,040	+				-0.7
138,040	+				-0.7
138,041	-				-1.1
138,041	-				-0.4
138,099	+				+0.0
138,100	-				+0.1
138,130	+				-0.6
138,130	+				-1.2
138,130	+				-0.7
138,130	+				+0.5
138,130	+				+1.1
138,130	+				-0.6
138,130	+				-0.2
138,130	+				-1.1
138,130	+				-0.5
138,130	+				-0.8
138,131	-				-0.4
138,131	-				-2.4
138,131	-				+0.3
138,131	-				-1.0
138,131	-				-1.6
138,131	-				-0.6
138,131	-				+0.0
138,131	-				+0.2
138,232	+		GFF3311	0.24	-0.2
138,232	+		GFF3311	0.24	+0.5
138,568	+		GFF3311	0.79	-1.0
138,568	+		GFF3311	0.79	-0.1
138,568	+		GFF3311	0.79	+1.5
138,568	+		GFF3311	0.79	-1.0
138,568	+		GFF3311	0.79	-0.9
138,569	-		GFF3311	0.79	+0.3
138,569	-		GFF3311	0.79	+0.7
138,569	-		GFF3311	0.79	-0.0
138,569	-		GFF3311	0.79	-1.7
138,569	-		GFF3311	0.79	-0.2
138,625	+		GFF3311	0.89	-1.3
138,625	+		GFF3311	0.89	-0.5
138,625	+		GFF3311	0.89	-0.1
138,625	+		GFF3311	0.89	+0.1
138,625	+		GFF3311	0.89	+1.9
138,625	+		GFF3311	0.89	+0.5
138,625	+		GFF3311	0.89	-0.1
138,626	-		GFF3311	0.89	-1.4
138,626	-		GFF3311	0.89	-0.5
138,670	+				+0.4
138,670	+				-0.6
138,671	-				-0.1
138,691	+				-0.4
138,692	-				-1.3
138,692	-				-0.5
138,692	-				+0.4
138,692	-				-0.9
138,870	+		GFF3312	0.12	+0.0
138,870	+		GFF3312	0.12	-0.1
138,871	-		GFF3312	0.12	+0.4
138,871	-		GFF3312	0.12	-0.3
138,871	-		GFF3312	0.12	+0.7
138,871	-		GFF3312	0.12	-0.1
138,871	-		GFF3312	0.12	+0.2
138,900	+		GFF3312	0.14	-0.9
138,901	-		GFF3312	0.14	-0.7
139,374	+		GFF3312	0.47	+1.4
139,374	+		GFF3312	0.47	-0.2
139,375	-		GFF3312	0.47	-0.5
139,375	-		GFF3312	0.47	-0.2
139,375	-		GFF3312	0.47	-0.1
139,386	+		GFF3312	0.47	-0.3
139,386	+		GFF3312	0.47	-0.3
139,386	+		GFF3312	0.47	-0.3
139,386	+		GFF3312	0.47	+0.2
139,387	-		GFF3312	0.47	-1.3
139,387	-		GFF3312	0.47	-0.9
139,387	-		GFF3312	0.47	+0.5
139,387	-		GFF3312	0.47	-1.1
139,387	-		GFF3312	0.47	-0.8
139,504	-		GFF3312	0.56	-0.8
139,688	+		GFF3312	0.68	+0.3