Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3307 and GFF3308 are separated by 176 nucleotides GFF3308 and GFF3309 overlap by 4 nucleotides
GFF3307 - FIG01161751: hypothetical protein, at 130,554 to 132,650
GFF3307
GFF3308 - Inner membrane protein CreD, at 132,827 to 134,290
GFF3308
GFF3309 - Beta-hexosaminidase (EC 3.2.1.52), at 134,287 to 135,579
GFF3309
Position (kb) 132
133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.022 kb on + strand, within GFF3307 at 132.058 kb on + strand, within GFF3307 at 132.058 kb on + strand, within GFF3307 at 132.058 kb on + strand, within GFF3307 at 132.058 kb on + strand, within GFF3307 at 132.059 kb on - strand, within GFF3307 at 132.059 kb on - strand, within GFF3307 at 132.092 kb on - strand, within GFF3307 at 132.211 kb on + strand, within GFF3307 at 132.212 kb on - strand, within GFF3307 at 132.212 kb on - strand, within GFF3307 at 132.212 kb on - strand, within GFF3307 at 132.319 kb on + strand, within GFF3307 at 132.319 kb on + strand, within GFF3307 at 132.320 kb on - strand, within GFF3307 at 132.355 kb on + strand, within GFF3307 at 132.355 kb on + strand, within GFF3307 at 132.355 kb on + strand, within GFF3307 at 132.356 kb on - strand, within GFF3307 at 132.356 kb on - strand, within GFF3307 at 132.356 kb on - strand, within GFF3307 at 132.356 kb on - strand, within GFF3307 at 132.467 kb on - strand at 132.467 kb on - strand at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.989 kb on + strand, within GFF3308 at 132.990 kb on - strand, within GFF3308 at 132.990 kb on - strand, within GFF3308 at 132.990 kb on - strand, within GFF3308 at 133.034 kb on + strand, within GFF3308 at 133.034 kb on + strand, within GFF3308 at 133.035 kb on - strand, within GFF3308 at 133.199 kb on + strand, within GFF3308 at 133.199 kb on + strand, within GFF3308 at 133.200 kb on - strand, within GFF3308 at 133.200 kb on - strand, within GFF3308 at 133.839 kb on - strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.865 kb on + strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.866 kb on - strand, within GFF3308 at 133.949 kb on + strand, within GFF3308 at 133.949 kb on + strand, within GFF3308 at 134.031 kb on - strand, within GFF3308 at 134.072 kb on + strand, within GFF3308 at 134.073 kb on - strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.138 kb on + strand, within GFF3308 at 134.139 kb on - strand, within GFF3308 at 134.139 kb on - strand, within GFF3308 at 134.150 kb on + strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.151 kb on - strand at 134.253 kb on - strand at 134.253 kb on - strand at 134.253 kb on - strand at 134.269 kb on + strand at 134.611 kb on + strand, within GFF3309 at 134.612 kb on - strand, within GFF3309 at 134.899 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.933 kb on + strand, within GFF3309 at 134.934 kb on - strand, within GFF3309 at 134.934 kb on - strand, within GFF3309 at 135.060 kb on - strand, within GFF3309
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 132,022 + GFF3307 0.70 +0.5 132,058 + GFF3307 0.72 +0.4 132,058 + GFF3307 0.72 +0.9 132,058 + GFF3307 0.72 -0.9 132,058 + GFF3307 0.72 -0.8 132,059 - GFF3307 0.72 -1.5 132,059 - GFF3307 0.72 -0.6 132,092 - GFF3307 0.73 -0.6 132,211 + GFF3307 0.79 +0.2 132,212 - GFF3307 0.79 -0.3 132,212 - GFF3307 0.79 +0.9 132,212 - GFF3307 0.79 -0.5 132,319 + GFF3307 0.84 -0.1 132,319 + GFF3307 0.84 -1.0 132,320 - GFF3307 0.84 +0.6 132,355 + GFF3307 0.86 -0.3 132,355 + GFF3307 0.86 -0.4 132,355 + GFF3307 0.86 -0.4 132,356 - GFF3307 0.86 -0.9 132,356 - GFF3307 0.86 -0.6 132,356 - GFF3307 0.86 -0.5 132,356 - GFF3307 0.86 -1.3 132,467 - -0.3 132,467 - -0.3 132,989 + GFF3308 0.11 -2.5 132,989 + GFF3308 0.11 -1.2 132,989 + GFF3308 0.11 -0.4 132,989 + GFF3308 0.11 -0.3 132,989 + GFF3308 0.11 +0.4 132,989 + GFF3308 0.11 -0.7 132,989 + GFF3308 0.11 -2.1 132,989 + GFF3308 0.11 -0.5 132,990 - GFF3308 0.11 -1.1 132,990 - GFF3308 0.11 -0.9 132,990 - GFF3308 0.11 +0.0 133,034 + GFF3308 0.14 -0.6 133,034 + GFF3308 0.14 +0.0 133,035 - GFF3308 0.14 +0.6 133,199 + GFF3308 0.25 -0.1 133,199 + GFF3308 0.25 +0.1 133,200 - GFF3308 0.25 -1.2 133,200 - GFF3308 0.25 +0.0 133,839 - GFF3308 0.69 -0.4 133,865 + GFF3308 0.71 -0.9 133,865 + GFF3308 0.71 +0.9 133,865 + GFF3308 0.71 -0.8 133,865 + GFF3308 0.71 -0.1 133,866 - GFF3308 0.71 -0.6 133,866 - GFF3308 0.71 +0.5 133,866 - GFF3308 0.71 +1.5 133,949 + GFF3308 0.77 +0.4 133,949 + GFF3308 0.77 -0.9 134,031 - GFF3308 0.82 +0.0 134,072 + GFF3308 0.85 +0.2 134,073 - GFF3308 0.85 -0.6 134,138 + GFF3308 0.90 -0.5 134,138 + GFF3308 0.90 -0.2 134,138 + GFF3308 0.90 -0.1 134,138 + GFF3308 0.90 +0.9 134,138 + GFF3308 0.90 +2.1 134,139 - GFF3308 0.90 -0.4 134,139 - GFF3308 0.90 -0.2 134,150 + -2.3 134,151 - -0.1 134,151 - -0.6 134,151 - +0.1 134,151 - -0.5 134,253 - +0.1 134,253 - +0.5 134,253 - +1.7 134,269 + +0.9 134,611 + GFF3309 0.25 -0.7 134,612 - GFF3309 0.25 +0.7 134,899 + GFF3309 0.47 +0.6 134,933 + GFF3309 0.50 +0.0 134,933 + GFF3309 0.50 +0.8 134,933 + GFF3309 0.50 +0.2 134,934 - GFF3309 0.50 -0.6 134,934 - GFF3309 0.50 +0.6 135,060 - GFF3309 0.60 -0.4
Or see this region's nucleotide sequence