Strain Fitness in Variovorax sp. SCN45 around GFF330

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF329 and GFF330 overlap by 4 nucleotidesGFF330 and GFF331 are separated by 133 nucleotides GFF329 - Nucleoside ABC transporter, ATP-binding protein, at 362,936 to 364,537 GFF329 GFF330 - Nucleoside ABC transporter, permease protein 1, at 364,534 to 365,601 GFF330 GFF331 - Nucleoside ABC transporter, permease protein 2, at 365,735 to 366,658 GFF331 Position (kb) 364 365 366Strain fitness (log2 ratio) -2 -1 0 1 2at 364.242 kb on - strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.313 kb on + strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.314 kb on - strand, within GFF329at 364.666 kb on + strand, within GFF330at 364.666 kb on + strand, within GFF330at 364.667 kb on - strand, within GFF330at 364.667 kb on - strand, within GFF330at 364.813 kb on + strand, within GFF330at 364.813 kb on + strand, within GFF330at 364.813 kb on + strand, within GFF330at 365.194 kb on + strand, within GFF330at 365.194 kb on + strand, within GFF330at 365.195 kb on - strand, within GFF330at 365.195 kb on - strand, within GFF330at 365.353 kb on + strand, within GFF330at 365.353 kb on + strand, within GFF330at 365.353 kb on + strand, within GFF330at 365.353 kb on + strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.354 kb on - strand, within GFF330at 365.386 kb on + strand, within GFF330at 365.386 kb on + strand, within GFF330at 365.386 kb on + strand, within GFF330at 365.512 kb on + strandat 365.512 kb on + strandat 365.512 kb on + strandat 365.512 kb on + strandat 365.512 kb on + strandat 365.512 kb on + strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.513 kb on - strandat 365.596 kb on - strandat 365.729 kb on + strandat 365.912 kb on + strand, within GFF331at 365.912 kb on + strand, within GFF331at 365.912 kb on + strand, within GFF331at 365.912 kb on + strand, within GFF331at 365.913 kb on - strand, within GFF331at 365.913 kb on - strand, within GFF331at 365.992 kb on + strand, within GFF331at 365.992 kb on + strand, within GFF331at 365.992 kb on + strand, within GFF331at 366.062 kb on + strand, within GFF331at 366.062 kb on + strand, within GFF331at 366.063 kb on - strand, within GFF331at 366.063 kb on - strand, within GFF331at 366.063 kb on - strand, within GFF331at 366.063 kb on - strand, within GFF331at 366.218 kb on + strand, within GFF331at 366.219 kb on - strand, within GFF331at 366.542 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.551 kb on + strand, within GFF331at 366.552 kb on - strand, within GFF331at 366.552 kb on - strand, within GFF331at 366.552 kb on - strand, within GFF331at 366.552 kb on - strand, within GFF331at 366.552 kb on - strand, within GFF331at 366.566 kb on + strandat 366.567 kb on - strandat 366.567 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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364,242 - GFF329 0.82 -0.5
364,313 + GFF329 0.86 -0.7
364,313 + GFF329 0.86 -0.9
364,313 + GFF329 0.86 +0.2
364,313 + GFF329 0.86 +0.8
364,313 + GFF329 0.86 -0.3
364,313 + GFF329 0.86 -1.4
364,314 - GFF329 0.86 +0.3
364,314 - GFF329 0.86 +0.5
364,314 - GFF329 0.86 -0.0
364,314 - GFF329 0.86 +0.5
364,314 - GFF329 0.86 -0.3
364,314 - GFF329 0.86 -0.3
364,314 - GFF329 0.86 -1.9
364,314 - GFF329 0.86 +1.1
364,314 - GFF329 0.86 -0.4
364,666 + GFF330 0.12 -0.5
364,666 + GFF330 0.12 +0.2
364,667 - GFF330 0.12 -0.1
364,667 - GFF330 0.12 -0.2
364,813 + GFF330 0.26 +0.1
364,813 + GFF330 0.26 +1.8
364,813 + GFF330 0.26 -1.0
365,194 + GFF330 0.62 -0.3
365,194 + GFF330 0.62 +1.7
365,195 - GFF330 0.62 +0.5
365,195 - GFF330 0.62 +0.7
365,353 + GFF330 0.77 -0.3
365,353 + GFF330 0.77 -0.7
365,353 + GFF330 0.77 +0.2
365,353 + GFF330 0.77 -1.0
365,354 - GFF330 0.77 -0.3
365,354 - GFF330 0.77 +1.1
365,354 - GFF330 0.77 +0.8
365,354 - GFF330 0.77 +0.9
365,354 - GFF330 0.77 +0.3
365,354 - GFF330 0.77 -1.0
365,354 - GFF330 0.77 +2.5
365,354 - GFF330 0.77 -0.4
365,354 - GFF330 0.77 +0.7
365,354 - GFF330 0.77 -0.5
365,386 + GFF330 0.80 +0.7
365,386 + GFF330 0.80 +0.8
365,386 + GFF330 0.80 +0.8
365,512 + +0.7
365,512 + -0.1
365,512 + -1.0
365,512 + +0.9
365,512 + +0.6
365,512 + +1.9
365,513 - -0.7
365,513 - -0.1
365,513 - +0.9
365,513 - +1.1
365,513 - +0.4
365,513 - -0.3
365,513 - -0.8
365,513 - +0.7
365,596 - +0.3
365,729 + -0.6
365,912 + GFF331 0.19 +0.2
365,912 + GFF331 0.19 -0.1
365,912 + GFF331 0.19 -0.3
365,912 + GFF331 0.19 -0.5
365,913 - GFF331 0.19 -0.5
365,913 - GFF331 0.19 +0.5
365,992 + GFF331 0.28 -0.1
365,992 + GFF331 0.28 +0.0
365,992 + GFF331 0.28 +0.7
366,062 + GFF331 0.35 -0.5
366,062 + GFF331 0.35 -0.7
366,063 - GFF331 0.35 -0.1
366,063 - GFF331 0.35 +0.8
366,063 - GFF331 0.35 -0.0
366,063 - GFF331 0.35 +0.7
366,218 + GFF331 0.52 +0.4
366,219 - GFF331 0.52 -0.8
366,542 + GFF331 0.87 +1.9
366,551 + GFF331 0.88 +0.1
366,551 + GFF331 0.88 +0.3
366,551 + GFF331 0.88 +0.5
366,551 + GFF331 0.88 +1.1
366,551 + GFF331 0.88 -0.1
366,551 + GFF331 0.88 -0.3
366,552 - GFF331 0.88 +0.6
366,552 - GFF331 0.88 +1.1
366,552 - GFF331 0.88 +0.1
366,552 - GFF331 0.88 +1.6
366,552 - GFF331 0.88 +0.2
366,566 + +1.0
366,567 - -0.5
366,567 - +1.0

Or see this region's nucleotide sequence