Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3258 and GFF3259 overlap by 29 nucleotides GFF3259 and GFF3260 overlap by 4 nucleotides
GFF3258 - hypothetical protein, at 76,364 to 79,288
GFF3258
GFF3259 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23), at 79,260 to 80,120
GFF3259
GFF3260 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 80,117 to 81,628
GFF3260
Position (kb)
79
80
81 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.423 kb on + strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.424 kb on - strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.712 kb on + strand, within GFF3258 at 78.713 kb on - strand, within GFF3258 at 78.713 kb on - strand, within GFF3258 at 78.990 kb on + strand, within GFF3258 at 78.991 kb on - strand, within GFF3258 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.348 kb on + strand, within GFF3259 at 79.349 kb on - strand, within GFF3259 at 79.451 kb on - strand, within GFF3259 at 79.510 kb on + strand, within GFF3259 at 79.510 kb on + strand, within GFF3259 at 79.511 kb on - strand, within GFF3259 at 79.511 kb on - strand, within GFF3259 at 79.570 kb on + strand, within GFF3259 at 79.570 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.603 kb on + strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.604 kb on - strand, within GFF3259 at 79.972 kb on + strand, within GFF3259 at 79.973 kb on - strand, within GFF3259 at 80.209 kb on - strand at 80.226 kb on + strand at 80.343 kb on + strand, within GFF3260 at 80.343 kb on + strand, within GFF3260 at 80.344 kb on - strand, within GFF3260 at 80.469 kb on + strand, within GFF3260 at 80.469 kb on + strand, within GFF3260 at 80.469 kb on + strand, within GFF3260 at 80.469 kb on + strand, within GFF3260 at 80.469 kb on + strand, within GFF3260 at 80.565 kb on + strand, within GFF3260 at 80.565 kb on + strand, within GFF3260 at 80.565 kb on + strand, within GFF3260 at 80.566 kb on - strand, within GFF3260 at 80.566 kb on - strand, within GFF3260 at 80.715 kb on + strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.716 kb on - strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.739 kb on + strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.740 kb on - strand, within GFF3260 at 80.997 kb on + strand, within GFF3260 at 80.997 kb on + strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 80.998 kb on - strand, within GFF3260 at 81.069 kb on + strand, within GFF3260 at 81.069 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.084 kb on + strand, within GFF3260 at 81.085 kb on - strand, within GFF3260 at 81.085 kb on - strand, within GFF3260 at 81.085 kb on - strand, within GFF3260
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 78,423 + GFF3258 0.70 -1.6 78,423 + GFF3258 0.70 +2.9 78,423 + GFF3258 0.70 +0.6 78,423 + GFF3258 0.70 -0.5 78,423 + GFF3258 0.70 -1.1 78,423 + GFF3258 0.70 -0.3 78,423 + GFF3258 0.70 -1.5 78,423 + GFF3258 0.70 -0.1 78,424 - GFF3258 0.70 +0.6 78,424 - GFF3258 0.70 -0.1 78,424 - GFF3258 0.70 +0.4 78,424 - GFF3258 0.70 -1.0 78,424 - GFF3258 0.70 -0.9 78,712 + GFF3258 0.80 -0.5 78,712 + GFF3258 0.80 -0.5 78,712 + GFF3258 0.80 -0.4 78,713 - GFF3258 0.80 +0.5 78,713 - GFF3258 0.80 -0.1 78,990 + GFF3258 0.90 +0.9 78,991 - GFF3258 0.90 -0.3 79,348 + GFF3259 0.10 +0.2 79,348 + GFF3259 0.10 +0.2 79,348 + GFF3259 0.10 +0.6 79,348 + GFF3259 0.10 +0.2 79,349 - GFF3259 0.10 +1.9 79,451 - GFF3259 0.22 -0.8 79,510 + GFF3259 0.29 -0.4 79,510 + GFF3259 0.29 +1.3 79,511 - GFF3259 0.29 -0.3 79,511 - GFF3259 0.29 -0.8 79,570 + GFF3259 0.36 -1.5 79,570 + GFF3259 0.36 -0.5 79,603 + GFF3259 0.40 +0.2 79,603 + GFF3259 0.40 +0.1 79,603 + GFF3259 0.40 +0.0 79,603 + GFF3259 0.40 +0.4 79,603 + GFF3259 0.40 -0.4 79,603 + GFF3259 0.40 -0.1 79,603 + GFF3259 0.40 -0.0 79,604 - GFF3259 0.40 -0.4 79,604 - GFF3259 0.40 -0.0 79,604 - GFF3259 0.40 -0.5 79,604 - GFF3259 0.40 +0.6 79,972 + GFF3259 0.83 +0.7 79,973 - GFF3259 0.83 +0.2 80,209 - -0.1 80,226 + -0.2 80,343 + GFF3260 0.15 -1.4 80,343 + GFF3260 0.15 -1.0 80,344 - GFF3260 0.15 -1.5 80,469 + GFF3260 0.23 +1.2 80,469 + GFF3260 0.23 -0.6 80,469 + GFF3260 0.23 -0.8 80,469 + GFF3260 0.23 +0.7 80,469 + GFF3260 0.23 -2.5 80,565 + GFF3260 0.30 -0.1 80,565 + GFF3260 0.30 -0.1 80,565 + GFF3260 0.30 -0.6 80,566 - GFF3260 0.30 -1.1 80,566 - GFF3260 0.30 +0.4 80,715 + GFF3260 0.40 +0.2 80,716 - GFF3260 0.40 -0.3 80,716 - GFF3260 0.40 -0.3 80,716 - GFF3260 0.40 +0.0 80,716 - GFF3260 0.40 +0.0 80,716 - GFF3260 0.40 +0.4 80,716 - GFF3260 0.40 +0.2 80,739 + GFF3260 0.41 -0.1 80,739 + GFF3260 0.41 +3.3 80,739 + GFF3260 0.41 +0.6 80,739 + GFF3260 0.41 -1.1 80,739 + GFF3260 0.41 +0.4 80,739 + GFF3260 0.41 -1.2 80,739 + GFF3260 0.41 +0.2 80,740 - GFF3260 0.41 +0.9 80,740 - GFF3260 0.41 -0.7 80,740 - GFF3260 0.41 -0.1 80,740 - GFF3260 0.41 -0.8 80,740 - GFF3260 0.41 -0.3 80,997 + GFF3260 0.58 -0.4 80,997 + GFF3260 0.58 +0.2 80,998 - GFF3260 0.58 -0.9 80,998 - GFF3260 0.58 +0.1 80,998 - GFF3260 0.58 +0.5 81,069 + GFF3260 0.63 -0.5 81,069 + GFF3260 0.63 -1.2 81,084 + GFF3260 0.64 -2.3 81,084 + GFF3260 0.64 +0.4 81,084 + GFF3260 0.64 +0.9 81,084 + GFF3260 0.64 +0.7 81,085 - GFF3260 0.64 +1.9 81,085 - GFF3260 0.64 -0.0 81,085 - GFF3260 0.64 -0.1
Or see this region's nucleotide sequence