Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3214 and GFF3215 overlap by 4 nucleotides GFF3215 and GFF3216 are separated by 188 nucleotides GFF3216 and GFF3217 are separated by 6 nucleotides GFF3217 and GFF3218 are separated by 2 nucleotides
GFF3214 - FIG138928: iron-regulated membrane protein, at 34,423 to 36,090
GFF3214
GFF3215 - no description, at 36,087 to 36,398
GFF3215
GFF3216 - hypothetical protein, at 36,587 to 37,069
GFF3216
GFF3217 - hypothetical protein, at 37,076 to 37,687
GFF3217
GFF3218 - Phage tail fiber, at 37,690 to 38,703
GFF3218
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1
2 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.630 kb on + strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.631 kb on - strand, within GFF3214 at 35.655 kb on - strand, within GFF3214 at 35.889 kb on - strand, within GFF3214 at 35.889 kb on - strand, within GFF3214 at 35.987 kb on + strand at 35.987 kb on + strand at 35.987 kb on + strand at 35.988 kb on - strand at 35.988 kb on - strand at 36.379 kb on + strand at 36.565 kb on + strand at 36.639 kb on + strand, within GFF3216 at 36.639 kb on + strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.640 kb on - strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.702 kb on + strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.703 kb on - strand, within GFF3216 at 36.846 kb on - strand, within GFF3216 at 37.077 kb on + strand at 37.077 kb on + strand at 37.078 kb on - strand at 37.078 kb on - strand at 37.201 kb on - strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.284 kb on + strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.285 kb on - strand, within GFF3217 at 37.318 kb on - strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.391 kb on + strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.392 kb on - strand, within GFF3217 at 37.448 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.501 kb on + strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.502 kb on - strand, within GFF3217 at 37.521 kb on + strand, within GFF3217 at 37.521 kb on + strand, within GFF3217 at 37.641 kb on + strand at 37.642 kb on + strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.642 kb on - strand at 37.665 kb on + strand at 37.666 kb on - strand at 37.694 kb on + strand at 37.695 kb on - strand at 37.712 kb on + strand at 37.712 kb on + strand at 37.713 kb on - strand at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.808 kb on + strand, within GFF3218 at 37.809 kb on - strand, within GFF3218 at 38.018 kb on + strand, within GFF3218 at 38.018 kb on + strand, within GFF3218 at 38.019 kb on - strand, within GFF3218 at 38.019 kb on - strand, within GFF3218 at 38.019 kb on - strand, within GFF3218
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 35,630 + GFF3214 0.72 -0.2 35,630 + GFF3214 0.72 -0.4 35,630 + GFF3214 0.72 +0.3 35,630 + GFF3214 0.72 +0.6 35,630 + GFF3214 0.72 +0.2 35,631 - GFF3214 0.72 -0.5 35,631 - GFF3214 0.72 -0.9 35,631 - GFF3214 0.72 -0.1 35,631 - GFF3214 0.72 -0.3 35,631 - GFF3214 0.72 -0.8 35,631 - GFF3214 0.72 -1.1 35,631 - GFF3214 0.72 +1.5 35,631 - GFF3214 0.72 -0.7 35,631 - GFF3214 0.72 +0.4 35,631 - GFF3214 0.72 +0.1 35,631 - GFF3214 0.72 -0.5 35,631 - GFF3214 0.72 -0.7 35,631 - GFF3214 0.72 +1.9 35,655 - GFF3214 0.74 -0.4 35,889 - GFF3214 0.88 -0.1 35,889 - GFF3214 0.88 +0.0 35,987 + -1.2 35,987 + -1.1 35,987 + -0.6 35,988 - -1.9 35,988 - -1.1 36,379 + -1.0 36,565 + -0.6 36,639 + GFF3216 0.11 +0.4 36,639 + GFF3216 0.11 -1.4 36,640 - GFF3216 0.11 +0.4 36,640 - GFF3216 0.11 +0.1 36,640 - GFF3216 0.11 +1.9 36,640 - GFF3216 0.11 -1.1 36,702 + GFF3216 0.24 -2.1 36,702 + GFF3216 0.24 -0.3 36,702 + GFF3216 0.24 -0.9 36,703 - GFF3216 0.24 -0.6 36,703 - GFF3216 0.24 -0.0 36,703 - GFF3216 0.24 -0.7 36,846 - GFF3216 0.54 -0.2 37,077 + -0.5 37,077 + -1.4 37,078 - +0.2 37,078 - -0.1 37,201 - GFF3217 0.20 -0.6 37,284 + GFF3217 0.34 -0.1 37,284 + GFF3217 0.34 -0.3 37,284 + GFF3217 0.34 -0.8 37,285 - GFF3217 0.34 -0.0 37,285 - GFF3217 0.34 -1.8 37,285 - GFF3217 0.34 +0.3 37,318 - GFF3217 0.40 +0.2 37,391 + GFF3217 0.51 +0.4 37,391 + GFF3217 0.51 -0.1 37,391 + GFF3217 0.51 -0.9 37,392 - GFF3217 0.52 -0.4 37,392 - GFF3217 0.52 -0.9 37,392 - GFF3217 0.52 -0.3 37,392 - GFF3217 0.52 -0.9 37,392 - GFF3217 0.52 -0.7 37,392 - GFF3217 0.52 +1.5 37,448 + GFF3217 0.61 -1.5 37,501 + GFF3217 0.69 -0.6 37,501 + GFF3217 0.69 -0.3 37,501 + GFF3217 0.69 -0.1 37,501 + GFF3217 0.69 -1.3 37,501 + GFF3217 0.69 +0.4 37,502 - GFF3217 0.70 -1.1 37,502 - GFF3217 0.70 +0.1 37,502 - GFF3217 0.70 -1.1 37,502 - GFF3217 0.70 -0.2 37,502 - GFF3217 0.70 -0.3 37,502 - GFF3217 0.70 +0.7 37,502 - GFF3217 0.70 +0.7 37,521 + GFF3217 0.73 -0.6 37,521 + GFF3217 0.73 +0.2 37,641 + -0.2 37,642 + +0.5 37,642 - -0.2 37,642 - -0.3 37,642 - +0.1 37,642 - -0.7 37,642 - -0.3 37,665 + -2.1 37,666 - -0.3 37,694 + -0.2 37,695 - -0.6 37,712 + +0.1 37,712 + -0.4 37,713 - +1.9 37,808 + GFF3218 0.12 -0.3 37,808 + GFF3218 0.12 -1.4 37,808 + GFF3218 0.12 +0.4 37,808 + GFF3218 0.12 -0.4 37,809 - GFF3218 0.12 +1.0 38,018 + GFF3218 0.32 -0.3 38,018 + GFF3218 0.32 +0.4 38,019 - GFF3218 0.32 -0.2 38,019 - GFF3218 0.32 -0.9 38,019 - GFF3218 0.32 -1.4
Or see this region's nucleotide sequence