Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3103 and GFF3104 are separated by 39 nucleotides GFF3104 and GFF3105 are separated by 12 nucleotides GFF3105 and GFF3106 are separated by 34 nucleotides GFF3106 and GFF3107 are separated by 17 nucleotides
GFF3103 - Cys-tRNA(Pro) deacylase YbaK, at 530,949 to 531,443
GFF3103
GFF3104 - no description, at 531,483 to 532,172
GFF3104
GFF3105 - Tas protein, an NADP(H)-dependent aldo-keto reductase, at 532,185 to 533,363
GFF3105
GFF3106 - Hydrolase, alpha/beta fold family, at 533,398 to 534,291
GFF3106
GFF3107 - Oxidoreductase, aldo/keto reductase family, at 534,309 to 535,319
GFF3107
Position (kb) 532
533
534 Strain fitness (log2 ratio) -2
-1
0
1
2 at 531.352 kb on + strand, within GFF3103 at 531.352 kb on + strand, within GFF3103 at 531.352 kb on + strand, within GFF3103 at 531.352 kb on + strand, within GFF3103 at 531.352 kb on + strand, within GFF3103 at 531.352 kb on + strand, within GFF3103 at 531.353 kb on - strand, within GFF3103 at 531.353 kb on - strand, within GFF3103 at 531.353 kb on - strand, within GFF3103 at 531.353 kb on - strand, within GFF3103 at 531.353 kb on - strand, within GFF3103 at 531.358 kb on + strand, within GFF3103 at 531.358 kb on + strand, within GFF3103 at 531.358 kb on + strand, within GFF3103 at 531.359 kb on - strand, within GFF3103 at 531.359 kb on - strand, within GFF3103 at 531.359 kb on - strand, within GFF3103 at 531.422 kb on + strand at 531.422 kb on + strand at 531.422 kb on + strand at 531.422 kb on + strand at 531.422 kb on + strand at 531.422 kb on + strand at 531.423 kb on - strand at 531.423 kb on - strand at 531.423 kb on - strand at 531.423 kb on - strand at 531.423 kb on - strand at 531.423 kb on - strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.513 kb on + strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.514 kb on - strand at 531.543 kb on + strand at 531.543 kb on + strand at 531.543 kb on + strand at 531.544 kb on - strand at 531.544 kb on - strand at 531.544 kb on - strand at 531.544 kb on - strand at 531.544 kb on - strand at 531.544 kb on - strand at 531.544 kb on - strand at 532.261 kb on + strand at 532.262 kb on - strand at 532.336 kb on + strand, within GFF3105 at 532.336 kb on + strand, within GFF3105 at 532.337 kb on - strand, within GFF3105 at 532.337 kb on - strand, within GFF3105 at 532.337 kb on - strand, within GFF3105 at 532.396 kb on + strand, within GFF3105 at 532.396 kb on + strand, within GFF3105 at 532.396 kb on + strand, within GFF3105 at 532.396 kb on + strand, within GFF3105 at 532.397 kb on - strand, within GFF3105 at 532.397 kb on - strand, within GFF3105 at 532.456 kb on + strand, within GFF3105 at 532.456 kb on + strand, within GFF3105 at 532.456 kb on + strand, within GFF3105 at 532.513 kb on + strand, within GFF3105 at 532.513 kb on + strand, within GFF3105 at 532.514 kb on - strand, within GFF3105 at 532.514 kb on - strand, within GFF3105 at 532.514 kb on - strand, within GFF3105 at 532.550 kb on - strand, within GFF3105 at 532.550 kb on - strand, within GFF3105 at 532.550 kb on - strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.624 kb on + strand, within GFF3105 at 532.625 kb on - strand, within GFF3105 at 532.625 kb on - strand, within GFF3105 at 532.625 kb on - strand, within GFF3105 at 532.625 kb on - strand, within GFF3105 at 532.625 kb on - strand, within GFF3105 at 532.811 kb on - strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.813 kb on + strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.814 kb on - strand, within GFF3105 at 532.861 kb on + strand, within GFF3105 at 533.062 kb on + strand, within GFF3105 at 533.062 kb on + strand, within GFF3105 at 533.062 kb on + strand, within GFF3105 at 533.062 kb on + strand, within GFF3105 at 533.063 kb on - strand, within GFF3105 at 533.063 kb on - strand, within GFF3105 at 533.089 kb on + strand, within GFF3105 at 533.089 kb on + strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.090 kb on - strand, within GFF3105 at 533.109 kb on - strand, within GFF3105 at 533.258 kb on + strand at 533.259 kb on - strand at 533.259 kb on - strand at 533.294 kb on + strand at 533.294 kb on + strand at 533.348 kb on + strand at 533.348 kb on + strand at 533.349 kb on - strand at 533.349 kb on - strand at 533.365 kb on + strand at 533.365 kb on + strand at 533.365 kb on + strand at 533.365 kb on + strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.366 kb on - strand at 533.419 kb on + strand at 533.419 kb on + strand at 533.420 kb on - strand at 533.420 kb on - strand at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.599 kb on + strand, within GFF3106 at 533.600 kb on - strand, within GFF3106 at 533.600 kb on - strand, within GFF3106 at 533.600 kb on - strand, within GFF3106 at 533.600 kb on - strand, within GFF3106 at 533.615 kb on - strand, within GFF3106 at 533.647 kb on + strand, within GFF3106 at 533.722 kb on + strand, within GFF3106 at 533.722 kb on + strand, within GFF3106 at 533.722 kb on + strand, within GFF3106 at 533.722 kb on + strand, within GFF3106 at 533.723 kb on - strand, within GFF3106 at 533.723 kb on - strand, within GFF3106 at 533.723 kb on - strand, within GFF3106 at 533.815 kb on + strand, within GFF3106 at 534.076 kb on + strand, within GFF3106 at 534.151 kb on + strand, within GFF3106 at 534.152 kb on - strand, within GFF3106 at 534.152 kb on - strand, within GFF3106
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 531,352 + GFF3103 0.81 -0.2 531,352 + GFF3103 0.81 -0.6 531,352 + GFF3103 0.81 -0.7 531,352 + GFF3103 0.81 +0.6 531,352 + GFF3103 0.81 -0.0 531,352 + GFF3103 0.81 +0.0 531,353 - GFF3103 0.82 -0.4 531,353 - GFF3103 0.82 +0.0 531,353 - GFF3103 0.82 -0.3 531,353 - GFF3103 0.82 -2.0 531,353 - GFF3103 0.82 -0.1 531,358 + GFF3103 0.83 -0.4 531,358 + GFF3103 0.83 -1.3 531,358 + GFF3103 0.83 -0.0 531,359 - GFF3103 0.83 -0.3 531,359 - GFF3103 0.83 -1.2 531,359 - GFF3103 0.83 +1.6 531,422 + +0.0 531,422 + -2.3 531,422 + -1.4 531,422 + -1.0 531,422 + -1.3 531,422 + -0.7 531,423 - -0.3 531,423 - -0.7 531,423 - -1.2 531,423 - +1.2 531,423 - +0.8 531,423 - +1.2 531,513 + +0.3 531,513 + +0.9 531,513 + -0.4 531,513 + -0.8 531,513 + -0.3 531,513 + -1.2 531,513 + +0.0 531,513 + -0.0 531,513 + -0.1 531,514 - -0.0 531,514 - -1.8 531,514 - -0.4 531,514 - +0.6 531,514 - -0.9 531,514 - -0.8 531,514 - -0.1 531,514 - +0.1 531,543 + +0.8 531,543 + +0.6 531,543 + -0.4 531,544 - +0.7 531,544 - -0.7 531,544 - -1.2 531,544 - -0.3 531,544 - +0.0 531,544 - -0.3 531,544 - -1.0 532,261 + +0.0 532,262 - -0.2 532,336 + GFF3105 0.13 -0.0 532,336 + GFF3105 0.13 -1.0 532,337 - GFF3105 0.13 -0.9 532,337 - GFF3105 0.13 +0.1 532,337 - GFF3105 0.13 +0.3 532,396 + GFF3105 0.18 +0.5 532,396 + GFF3105 0.18 +0.0 532,396 + GFF3105 0.18 +0.4 532,396 + GFF3105 0.18 -0.1 532,397 - GFF3105 0.18 -0.5 532,397 - GFF3105 0.18 -1.5 532,456 + GFF3105 0.23 -0.8 532,456 + GFF3105 0.23 +1.1 532,456 + GFF3105 0.23 -0.2 532,513 + GFF3105 0.28 +1.4 532,513 + GFF3105 0.28 -0.6 532,514 - GFF3105 0.28 +0.4 532,514 - GFF3105 0.28 +0.0 532,514 - GFF3105 0.28 -1.1 532,550 - GFF3105 0.31 +0.4 532,550 - GFF3105 0.31 -0.1 532,550 - GFF3105 0.31 -0.4 532,624 + GFF3105 0.37 +0.7 532,624 + GFF3105 0.37 -1.1 532,624 + GFF3105 0.37 -1.4 532,624 + GFF3105 0.37 -1.2 532,624 + GFF3105 0.37 -0.0 532,624 + GFF3105 0.37 +0.8 532,625 - GFF3105 0.37 +0.2 532,625 - GFF3105 0.37 -0.2 532,625 - GFF3105 0.37 -0.4 532,625 - GFF3105 0.37 -0.1 532,625 - GFF3105 0.37 -0.0 532,811 - GFF3105 0.53 +0.3 532,813 + GFF3105 0.53 +0.3 532,813 + GFF3105 0.53 -0.0 532,813 + GFF3105 0.53 -0.3 532,813 + GFF3105 0.53 -0.2 532,813 + GFF3105 0.53 +0.4 532,813 + GFF3105 0.53 +0.6 532,813 + GFF3105 0.53 -0.2 532,813 + GFF3105 0.53 +0.5 532,813 + GFF3105 0.53 -0.0 532,813 + GFF3105 0.53 +0.4 532,813 + GFF3105 0.53 -0.1 532,813 + GFF3105 0.53 +0.5 532,813 + GFF3105 0.53 -1.0 532,813 + GFF3105 0.53 +1.9 532,813 + GFF3105 0.53 +0.3 532,813 + GFF3105 0.53 -0.4 532,813 + GFF3105 0.53 -0.5 532,814 - GFF3105 0.53 +0.3 532,814 - GFF3105 0.53 +0.8 532,814 - GFF3105 0.53 +0.4 532,814 - GFF3105 0.53 -0.3 532,814 - GFF3105 0.53 -0.6 532,814 - GFF3105 0.53 +0.6 532,814 - GFF3105 0.53 +0.1 532,814 - GFF3105 0.53 +0.3 532,814 - GFF3105 0.53 +0.1 532,814 - GFF3105 0.53 -0.3 532,814 - GFF3105 0.53 -0.3 532,814 - GFF3105 0.53 -1.5 532,814 - GFF3105 0.53 -0.5 532,814 - GFF3105 0.53 +0.0 532,814 - GFF3105 0.53 +0.0 532,814 - GFF3105 0.53 +0.7 532,814 - GFF3105 0.53 -0.3 532,814 - GFF3105 0.53 -0.2 532,861 + GFF3105 0.57 -0.6 533,062 + GFF3105 0.74 -0.1 533,062 + GFF3105 0.74 -1.5 533,062 + GFF3105 0.74 -0.4 533,062 + GFF3105 0.74 +2.0 533,063 - GFF3105 0.74 +0.0 533,063 - GFF3105 0.74 -0.5 533,089 + GFF3105 0.77 +1.0 533,089 + GFF3105 0.77 -0.2 533,090 - GFF3105 0.77 +1.3 533,090 - GFF3105 0.77 +0.6 533,090 - GFF3105 0.77 -0.0 533,090 - GFF3105 0.77 -0.3 533,090 - GFF3105 0.77 -0.5 533,090 - GFF3105 0.77 -1.0 533,090 - GFF3105 0.77 +0.0 533,090 - GFF3105 0.77 +0.7 533,090 - GFF3105 0.77 +0.0 533,090 - GFF3105 0.77 -0.3 533,090 - GFF3105 0.77 +1.6 533,090 - GFF3105 0.77 -1.0 533,090 - GFF3105 0.77 +0.9 533,090 - GFF3105 0.77 -1.4 533,109 - GFF3105 0.78 -0.6 533,258 + +1.2 533,259 - -0.0 533,259 - -1.4 533,294 + +1.2 533,294 + +0.2 533,348 + +1.8 533,348 + -0.7 533,349 - -1.9 533,349 - +1.2 533,365 + +0.1 533,365 + +0.8 533,365 + -0.8 533,365 + -1.2 533,366 - +0.4 533,366 - +0.6 533,366 - -0.1 533,366 - -0.5 533,366 - -0.3 533,366 - -1.7 533,366 - +1.4 533,366 - -0.2 533,419 + +0.5 533,419 + -0.7 533,420 - -0.0 533,420 - -0.7 533,599 + GFF3106 0.22 +0.6 533,599 + GFF3106 0.22 +0.5 533,599 + GFF3106 0.22 -0.5 533,599 + GFF3106 0.22 -0.4 533,599 + GFF3106 0.22 +0.3 533,599 + GFF3106 0.22 +0.6 533,599 + GFF3106 0.22 -0.0 533,600 - GFF3106 0.23 +0.2 533,600 - GFF3106 0.23 -0.5 533,600 - GFF3106 0.23 -0.4 533,600 - GFF3106 0.23 -0.4 533,615 - GFF3106 0.24 -0.1 533,647 + GFF3106 0.28 -0.4 533,722 + GFF3106 0.36 +0.3 533,722 + GFF3106 0.36 -0.4 533,722 + GFF3106 0.36 +0.5 533,722 + GFF3106 0.36 -0.1 533,723 - GFF3106 0.36 -0.3 533,723 - GFF3106 0.36 +0.3 533,723 - GFF3106 0.36 -0.7 533,815 + GFF3106 0.47 +0.8 534,076 + GFF3106 0.76 -0.5 534,151 + GFF3106 0.84 -1.0 534,152 - GFF3106 0.84 -0.7 534,152 - GFF3106 0.84 +0.0
Or see this region's nucleotide sequence