Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3042 and GFF3043 are separated by 4 nucleotides GFF3043 and GFF3044 are separated by 69 nucleotides GFF3044 and GFF3045 are separated by 884 nucleotides
GFF3042 - hypothetical protein, at 476,143 to 476,913
GFF3042
GFF3043 - Response regulator BaeR, at 476,918 to 477,607
GFF3043
GFF3044 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 477,677 to 480,034
GFF3044
GFF3045 - Glutamine ABC transporter, substrate-binding protein GlnH, at 480,919 to 481,761
GFF3045
Position (kb) 477
478
479
480
481 Strain fitness (log2 ratio) -2
-1
0
1 at 476.698 kb on + strand, within GFF3042 at 476.699 kb on - strand, within GFF3042 at 476.699 kb on - strand, within GFF3042 at 476.744 kb on - strand, within GFF3042 at 477.210 kb on - strand, within GFF3043 at 477.210 kb on - strand, within GFF3043 at 477.210 kb on - strand, within GFF3043 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.386 kb on - strand, within GFF3044 at 478.397 kb on + strand, within GFF3044 at 478.398 kb on - strand, within GFF3044 at 478.401 kb on - strand, within GFF3044 at 478.401 kb on - strand, within GFF3044 at 478.502 kb on + strand, within GFF3044 at 478.503 kb on - strand, within GFF3044 at 478.664 kb on + strand, within GFF3044 at 478.790 kb on + strand, within GFF3044 at 478.818 kb on - strand, within GFF3044 at 478.847 kb on + strand, within GFF3044 at 478.848 kb on - strand, within GFF3044 at 478.896 kb on - strand, within GFF3044 at 478.896 kb on - strand, within GFF3044 at 478.923 kb on - strand, within GFF3044 at 479.041 kb on + strand, within GFF3044 at 479.051 kb on + strand, within GFF3044 at 479.280 kb on - strand, within GFF3044 at 479.582 kb on + strand, within GFF3044 at 479.583 kb on - strand, within GFF3044 at 479.598 kb on - strand, within GFF3044 at 479.775 kb on - strand, within GFF3044 at 479.823 kb on - strand at 479.998 kb on + strand at 479.999 kb on - strand at 479.999 kb on - strand at 479.999 kb on - strand at 480.020 kb on - strand at 480.303 kb on - strand at 480.303 kb on - strand at 480.399 kb on - strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.401 kb on + strand at 480.417 kb on + strand at 480.686 kb on + strand at 480.687 kb on - strand at 480.717 kb on + strand at 480.918 kb on - strand at 480.983 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 476,698 + GFF3042 0.72 +0.2 476,699 - GFF3042 0.72 +0.3 476,699 - GFF3042 0.72 -0.2 476,744 - GFF3042 0.78 -0.1 477,210 - GFF3043 0.42 -0.3 477,210 - GFF3043 0.42 -0.7 477,210 - GFF3043 0.42 -0.2 478,152 - GFF3044 0.20 -0.8 478,152 - GFF3044 0.20 -1.3 478,152 - GFF3044 0.20 -0.6 478,152 - GFF3044 0.20 -1.1 478,386 - GFF3044 0.30 +0.3 478,397 + GFF3044 0.31 -0.4 478,398 - GFF3044 0.31 -0.2 478,401 - GFF3044 0.31 -1.5 478,401 - GFF3044 0.31 -0.4 478,502 + GFF3044 0.35 -1.4 478,503 - GFF3044 0.35 -0.3 478,664 + GFF3044 0.42 -2.0 478,790 + GFF3044 0.47 -0.8 478,818 - GFF3044 0.48 +0.3 478,847 + GFF3044 0.50 -0.4 478,848 - GFF3044 0.50 +0.7 478,896 - GFF3044 0.52 +0.6 478,896 - GFF3044 0.52 -0.4 478,923 - GFF3044 0.53 -0.1 479,041 + GFF3044 0.58 -0.4 479,051 + GFF3044 0.58 -0.7 479,280 - GFF3044 0.68 +0.2 479,582 + GFF3044 0.81 +0.9 479,583 - GFF3044 0.81 +0.6 479,598 - GFF3044 0.81 -0.6 479,775 - GFF3044 0.89 -1.3 479,823 - -2.1 479,998 + -1.0 479,999 - -1.1 479,999 - -0.1 479,999 - -0.1 480,020 - +0.5 480,303 - +0.9 480,303 - +1.6 480,399 - +1.2 480,401 + +0.1 480,401 + -0.2 480,401 + -0.7 480,401 + -0.7 480,401 + +1.1 480,417 + -0.7 480,686 + -0.3 480,687 - -0.5 480,717 + +1.0 480,918 - +0.3 480,983 + -0.5
Or see this region's nucleotide sequence