Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2993 and GFF2994 are separated by 115 nucleotides GFF2994 and GFF2995 are separated by 6 nucleotides GFF2995 and GFF2996 are separated by 14 nucleotides
GFF2993 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 424,114 to 425,544
GFF2993
GFF2994 - Transcriptional regulator, LysR family, at 425,660 to 426,610
GFF2994
GFF2995 - hypothetical protein, at 426,617 to 427,279
GFF2995
GFF2996 - Thiol-disulfide isomerase and thioredoxins, at 427,294 to 427,677
GFF2996
Position (kb)
425
426
427 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 425.090 kb on + strand, within GFF2993 at 425.090 kb on + strand, within GFF2993 at 425.091 kb on - strand, within GFF2993 at 425.091 kb on - strand, within GFF2993 at 425.183 kb on + strand, within GFF2993 at 425.184 kb on - strand, within GFF2993 at 425.273 kb on + strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.274 kb on - strand, within GFF2993 at 425.411 kb on + strand at 425.411 kb on + strand at 425.412 kb on - strand at 425.412 kb on - strand at 425.508 kb on - strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.581 kb on + strand at 425.582 kb on - strand at 425.582 kb on - strand at 425.582 kb on - strand at 425.625 kb on + strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.626 kb on - strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.747 kb on + strand at 425.748 kb on - strand at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.110 kb on + strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.111 kb on - strand, within GFF2994 at 426.141 kb on + strand, within GFF2994 at 426.142 kb on - strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.167 kb on + strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.168 kb on - strand, within GFF2994 at 426.315 kb on - strand, within GFF2994 at 426.784 kb on - strand, within GFF2995 at 426.784 kb on - strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.843 kb on + strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.844 kb on - strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.951 kb on + strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 426.952 kb on - strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.014 kb on + strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.015 kb on - strand, within GFF2995 at 427.029 kb on + strand, within GFF2995 at 427.029 kb on + strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.030 kb on - strand, within GFF2995 at 427.116 kb on + strand, within GFF2995 at 427.116 kb on + strand, within GFF2995 at 427.117 kb on - strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.122 kb on + strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.123 kb on - strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.184 kb on + strand, within GFF2995 at 427.185 kb on - strand, within GFF2995
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 425,090 + GFF2993 0.68 +0.2 425,090 + GFF2993 0.68 -1.2 425,091 - GFF2993 0.68 -0.3 425,091 - GFF2993 0.68 +0.2 425,183 + GFF2993 0.75 -1.6 425,184 - GFF2993 0.75 -0.4 425,273 + GFF2993 0.81 +0.1 425,274 - GFF2993 0.81 +0.7 425,274 - GFF2993 0.81 +2.0 425,274 - GFF2993 0.81 -0.7 425,274 - GFF2993 0.81 -1.9 425,274 - GFF2993 0.81 +0.8 425,274 - GFF2993 0.81 +0.2 425,274 - GFF2993 0.81 -0.8 425,274 - GFF2993 0.81 -2.4 425,274 - GFF2993 0.81 -0.2 425,274 - GFF2993 0.81 -0.2 425,411 + +1.9 425,411 + -0.9 425,412 - +0.4 425,412 - -0.9 425,508 - +0.3 425,581 + +0.0 425,581 + -1.2 425,581 + +0.0 425,581 + -0.3 425,581 + +0.9 425,582 - -1.4 425,582 - -0.7 425,582 - -0.4 425,625 + +0.0 425,626 - -1.3 425,626 - -0.8 425,626 - -0.6 425,626 - -1.0 425,747 + -0.1 425,747 + +1.2 425,747 + +0.5 425,747 + -0.6 425,747 + -1.1 425,747 + -0.6 425,747 + +0.4 425,747 + -1.1 425,747 + +0.2 425,747 + -2.9 425,748 - -0.2 426,110 + GFF2994 0.47 -2.4 426,110 + GFF2994 0.47 +0.0 426,110 + GFF2994 0.47 -0.4 426,110 + GFF2994 0.47 -1.9 426,110 + GFF2994 0.47 -0.8 426,111 - GFF2994 0.47 -1.2 426,111 - GFF2994 0.47 -2.6 426,111 - GFF2994 0.47 +0.6 426,111 - GFF2994 0.47 -0.4 426,111 - GFF2994 0.47 -1.0 426,111 - GFF2994 0.47 -1.2 426,141 + GFF2994 0.51 -0.6 426,142 - GFF2994 0.51 -0.7 426,167 + GFF2994 0.53 +0.8 426,167 + GFF2994 0.53 -0.1 426,167 + GFF2994 0.53 -0.4 426,168 - GFF2994 0.53 +0.0 426,168 - GFF2994 0.53 -0.2 426,168 - GFF2994 0.53 -0.0 426,315 - GFF2994 0.69 -0.4 426,784 - GFF2995 0.25 -0.1 426,784 - GFF2995 0.25 -0.7 426,843 + GFF2995 0.34 -0.2 426,843 + GFF2995 0.34 -0.4 426,843 + GFF2995 0.34 -0.7 426,843 + GFF2995 0.34 -0.1 426,843 + GFF2995 0.34 +0.6 426,843 + GFF2995 0.34 -0.4 426,843 + GFF2995 0.34 -0.9 426,843 + GFF2995 0.34 +0.0 426,843 + GFF2995 0.34 -0.4 426,843 + GFF2995 0.34 +0.3 426,844 - GFF2995 0.34 -0.2 426,844 - GFF2995 0.34 -0.2 426,844 - GFF2995 0.34 -0.6 426,844 - GFF2995 0.34 +0.0 426,844 - GFF2995 0.34 -0.5 426,844 - GFF2995 0.34 -0.2 426,844 - GFF2995 0.34 +0.3 426,844 - GFF2995 0.34 -0.6 426,844 - GFF2995 0.34 +0.5 426,951 + GFF2995 0.50 -1.2 426,951 + GFF2995 0.50 -1.3 426,951 + GFF2995 0.50 -0.6 426,951 + GFF2995 0.50 -1.4 426,951 + GFF2995 0.50 +0.0 426,951 + GFF2995 0.50 -0.4 426,951 + GFF2995 0.50 +0.0 426,951 + GFF2995 0.50 +0.4 426,952 - GFF2995 0.51 -0.7 426,952 - GFF2995 0.51 +0.6 426,952 - GFF2995 0.51 -0.3 426,952 - GFF2995 0.51 -0.4 426,952 - GFF2995 0.51 +0.1 426,952 - GFF2995 0.51 -0.2 426,952 - GFF2995 0.51 +1.3 426,952 - GFF2995 0.51 +0.1 427,014 + GFF2995 0.60 -1.6 427,014 + GFF2995 0.60 -0.7 427,014 + GFF2995 0.60 -0.1 427,014 + GFF2995 0.60 -0.9 427,014 + GFF2995 0.60 -1.4 427,015 - GFF2995 0.60 -0.1 427,015 - GFF2995 0.60 -1.0 427,015 - GFF2995 0.60 -0.7 427,029 + GFF2995 0.62 -0.4 427,029 + GFF2995 0.62 +0.0 427,030 - GFF2995 0.62 -0.1 427,030 - GFF2995 0.62 -0.6 427,030 - GFF2995 0.62 -0.8 427,116 + GFF2995 0.75 -0.3 427,116 + GFF2995 0.75 -0.8 427,117 - GFF2995 0.75 +0.2 427,122 + GFF2995 0.76 -0.1 427,122 + GFF2995 0.76 -0.8 427,122 + GFF2995 0.76 -0.9 427,122 + GFF2995 0.76 -0.2 427,122 + GFF2995 0.76 +0.9 427,122 + GFF2995 0.76 +0.6 427,122 + GFF2995 0.76 -2.5 427,122 + GFF2995 0.76 -0.2 427,122 + GFF2995 0.76 +0.6 427,122 + GFF2995 0.76 -0.4 427,122 + GFF2995 0.76 -1.2 427,122 + GFF2995 0.76 -0.7 427,122 + GFF2995 0.76 +0.3 427,122 + GFF2995 0.76 -0.5 427,122 + GFF2995 0.76 -1.6 427,122 + GFF2995 0.76 -0.1 427,122 + GFF2995 0.76 -1.0 427,122 + GFF2995 0.76 -0.6 427,122 + GFF2995 0.76 -0.0 427,122 + GFF2995 0.76 -0.3 427,123 - GFF2995 0.76 -0.5 427,123 - GFF2995 0.76 -1.3 427,123 - GFF2995 0.76 -0.4 427,123 - GFF2995 0.76 +0.6 427,123 - GFF2995 0.76 +0.4 427,123 - GFF2995 0.76 -0.7 427,123 - GFF2995 0.76 -1.5 427,123 - GFF2995 0.76 -0.5 427,123 - GFF2995 0.76 -0.5 427,123 - GFF2995 0.76 -0.7 427,123 - GFF2995 0.76 -0.0 427,123 - GFF2995 0.76 +0.2 427,123 - GFF2995 0.76 +0.0 427,123 - GFF2995 0.76 -0.5 427,123 - GFF2995 0.76 -0.2 427,123 - GFF2995 0.76 -0.4 427,123 - GFF2995 0.76 +0.6 427,184 + GFF2995 0.86 +0.6 427,184 + GFF2995 0.86 -0.3 427,184 + GFF2995 0.86 -2.1 427,185 - GFF2995 0.86 -0.9
Or see this region's nucleotide sequence