Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2969 and GFF2970 overlap by 4 nucleotides GFF2970 and GFF2971 are separated by 130 nucleotides
GFF2969 - acyl-CoA synthetase (NDP forming type), at 400,055 to 402,211
GFF2969
GFF2970 - BUG/TctC family periplasmic protein, at 402,208 to 403,194
GFF2970
GFF2971 - Transcriptional regulator, LysR family, at 403,325 to 404,290
GFF2971
Position (kb) 402
403
404 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 401.266 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.515 kb on - strand, within GFF2969 at 401.659 kb on - strand, within GFF2969 at 401.736 kb on + strand, within GFF2969 at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.069 kb on + strand at 402.070 kb on - strand at 402.070 kb on - strand at 402.311 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.832 kb on + strand, within GFF2970 at 402.833 kb on - strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.870 kb on - strand, within GFF2970 at 402.917 kb on + strand, within GFF2970 at 403.101 kb on - strand at 403.101 kb on - strand at 403.262 kb on + strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.274 kb on + strand at 403.276 kb on + strand at 403.289 kb on - strand at 403.312 kb on - strand at 403.312 kb on - strand at 403.402 kb on + strand at 403.402 kb on + strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.582 kb on - strand, within GFF2971 at 404.053 kb on - strand, within GFF2971 at 404.072 kb on + strand, within GFF2971 at 404.073 kb on - strand, within GFF2971 at 404.138 kb on - strand, within GFF2971 at 404.150 kb on + strand, within GFF2971
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 401,266 - GFF2969 0.56 -0.8 401,515 - GFF2969 0.68 -1.4 401,515 - GFF2969 0.68 +0.0 401,659 - GFF2969 0.74 -0.4 401,736 + GFF2969 0.78 -0.1 402,069 + -0.4 402,069 + -0.5 402,069 + +0.0 402,069 + -0.4 402,069 + -0.3 402,070 - -0.2 402,070 - -0.7 402,311 + GFF2970 0.10 -0.4 402,375 + GFF2970 0.17 +0.1 402,375 + GFF2970 0.17 -0.6 402,375 + GFF2970 0.17 +0.6 402,375 + GFF2970 0.17 +0.0 402,376 - GFF2970 0.17 +0.2 402,376 - GFF2970 0.17 -0.9 402,376 - GFF2970 0.17 +1.6 402,376 - GFF2970 0.17 -0.1 402,471 + GFF2970 0.27 -0.5 402,471 + GFF2970 0.27 +0.7 402,471 + GFF2970 0.27 -0.5 402,471 + GFF2970 0.27 +0.9 402,472 - GFF2970 0.27 -1.0 402,472 - GFF2970 0.27 -0.1 402,472 - GFF2970 0.27 -0.9 402,472 - GFF2970 0.27 +0.3 402,560 + GFF2970 0.36 -0.5 402,560 + GFF2970 0.36 +0.6 402,560 + GFF2970 0.36 +1.2 402,560 + GFF2970 0.36 -0.5 402,560 + GFF2970 0.36 +1.1 402,561 - GFF2970 0.36 +0.5 402,561 - GFF2970 0.36 -0.3 402,561 - GFF2970 0.36 -0.5 402,561 - GFF2970 0.36 +0.0 402,671 + GFF2970 0.47 -0.4 402,671 + GFF2970 0.47 -0.1 402,672 - GFF2970 0.47 -0.3 402,672 - GFF2970 0.47 -3.1 402,832 + GFF2970 0.63 -0.3 402,833 - GFF2970 0.63 -0.7 402,854 + GFF2970 0.65 -0.4 402,854 + GFF2970 0.65 -0.1 402,869 + GFF2970 0.67 -0.4 402,869 + GFF2970 0.67 -0.8 402,869 + GFF2970 0.67 -1.0 402,870 - GFF2970 0.67 -1.0 402,917 + GFF2970 0.72 -0.4 403,101 - +0.6 403,101 - +0.0 403,262 + +1.3 403,263 - +0.0 403,263 - -0.1 403,263 - -0.4 403,274 + +0.9 403,276 + -0.9 403,289 - +0.2 403,312 - -0.9 403,312 - -0.8 403,402 + +0.0 403,402 + -0.8 403,403 - -1.4 403,403 - +0.6 403,403 - -0.6 403,582 - GFF2971 0.27 -0.7 404,053 - GFF2971 0.75 -0.4 404,072 + GFF2971 0.77 +1.0 404,073 - GFF2971 0.77 -0.1 404,138 - GFF2971 0.84 -1.0 404,150 + GFF2971 0.85 -1.4
Or see this region's nucleotide sequence