Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2935 and GFF2936 are separated by 0 nucleotides GFF2936 and GFF2937 are separated by 39 nucleotides GFF2937 and GFF2938 are separated by 114 nucleotides GFF2938 and GFF2939 are separated by 8 nucleotides
GFF2935 - FIG004684: SpoVR-like protein, at 362,218 to 363,741
GFF2935
GFF2936 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 363,742 to 364,182
GFF2936
GFF2937 - Transcriptional regulator, LysR family, at 364,222 to 365,190
GFF2937
GFF2938 - Rieske (2Fe-2S) domain protein, at 365,305 to 365,976
GFF2938
GFF2939 - hypothetical protein, at 365,985 to 366,830
GFF2939
Position (kb) 364
365
366 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 363.264 kb on + strand, within GFF2935 at 363.328 kb on + strand, within GFF2935 at 363.328 kb on + strand, within GFF2935 at 363.328 kb on + strand, within GFF2935 at 363.328 kb on + strand, within GFF2935 at 363.329 kb on - strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.505 kb on + strand, within GFF2935 at 363.506 kb on - strand, within GFF2935 at 363.506 kb on - strand, within GFF2935 at 363.506 kb on - strand, within GFF2935 at 363.506 kb on - strand, within GFF2935 at 363.506 kb on - strand, within GFF2935 at 363.717 kb on + strand at 363.718 kb on - strand at 363.718 kb on - strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.736 kb on + strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.737 kb on - strand at 363.739 kb on + strand at 363.739 kb on + strand at 363.739 kb on + strand at 363.739 kb on + strand at 363.739 kb on + strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.740 kb on - strand at 363.773 kb on + strand at 363.773 kb on + strand at 363.774 kb on - strand at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.824 kb on + strand, within GFF2936 at 363.825 kb on - strand, within GFF2936 at 363.825 kb on - strand, within GFF2936 at 363.825 kb on - strand, within GFF2936 at 363.935 kb on + strand, within GFF2936 at 363.935 kb on + strand, within GFF2936 at 363.935 kb on + strand, within GFF2936 at 363.935 kb on + strand, within GFF2936 at 363.936 kb on - strand, within GFF2936 at 364.115 kb on + strand, within GFF2936 at 364.115 kb on + strand, within GFF2936 at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.191 kb on + strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.192 kb on - strand at 364.223 kb on + strand at 364.223 kb on + strand at 364.223 kb on + strand at 364.224 kb on - strand at 364.224 kb on - strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.271 kb on + strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.272 kb on - strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.274 kb on + strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.275 kb on - strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.280 kb on + strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.281 kb on - strand at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.346 kb on + strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.347 kb on - strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.370 kb on + strand, within GFF2937 at 364.371 kb on - strand, within GFF2937 at 364.371 kb on - strand, within GFF2937 at 364.371 kb on - strand, within GFF2937 at 364.371 kb on - strand, within GFF2937 at 364.371 kb on - strand, within GFF2937 at 364.661 kb on + strand, within GFF2937 at 364.661 kb on + strand, within GFF2937 at 364.662 kb on - strand, within GFF2937 at 364.662 kb on - strand, within GFF2937 at 364.662 kb on - strand, within GFF2937 at 364.685 kb on + strand, within GFF2937 at 364.685 kb on + strand, within GFF2937 at 364.685 kb on + strand, within GFF2937 at 364.686 kb on - strand, within GFF2937 at 364.686 kb on - strand, within GFF2937 at 364.686 kb on - strand, within GFF2937 at 364.686 kb on - strand, within GFF2937 at 364.686 kb on - strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.748 kb on + strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.749 kb on - strand, within GFF2937 at 364.750 kb on - strand, within GFF2937 at 365.260 kb on + strand at 365.261 kb on - strand at 365.261 kb on - strand at 365.261 kb on - strand at 365.261 kb on - strand at 365.518 kb on + strand, within GFF2938 at 365.518 kb on + strand, within GFF2938 at 365.519 kb on - strand, within GFF2938 at 365.533 kb on + strand, within GFF2938 at 365.533 kb on + strand, within GFF2938 at 365.534 kb on - strand, within GFF2938 at 366.159 kb on + strand, within GFF2939 at 366.165 kb on + strand, within GFF2939 at 366.166 kb on - strand, within GFF2939
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 363,264 + GFF2935 0.69 +1.0 363,328 + GFF2935 0.73 +0.3 363,328 + GFF2935 0.73 -1.7 363,328 + GFF2935 0.73 -0.5 363,328 + GFF2935 0.73 -0.9 363,329 - GFF2935 0.73 -0.1 363,505 + GFF2935 0.84 +1.0 363,505 + GFF2935 0.84 +0.2 363,505 + GFF2935 0.84 -1.4 363,505 + GFF2935 0.84 +0.2 363,505 + GFF2935 0.84 -0.1 363,505 + GFF2935 0.84 +0.2 363,506 - GFF2935 0.85 -0.3 363,506 - GFF2935 0.85 -2.0 363,506 - GFF2935 0.85 +1.2 363,506 - GFF2935 0.85 -0.3 363,506 - GFF2935 0.85 -0.1 363,717 + -0.1 363,718 - +2.2 363,718 - +0.3 363,736 + +1.6 363,736 + +0.3 363,736 + +0.4 363,736 + -0.2 363,736 + +0.2 363,736 + +1.1 363,736 + +0.9 363,736 + +0.6 363,736 + +0.5 363,736 + +1.2 363,736 + +1.6 363,736 + -1.0 363,736 + -0.1 363,737 - +0.3 363,737 - +0.0 363,737 - +0.3 363,737 - -0.1 363,737 - -0.7 363,737 - -0.4 363,737 - +0.9 363,737 - -0.2 363,737 - -0.1 363,739 + -0.7 363,739 + +0.1 363,739 + +0.1 363,739 + -0.0 363,739 + +0.2 363,740 - -0.1 363,740 - +0.6 363,740 - +0.4 363,740 - -0.4 363,740 - -0.7 363,740 - -0.1 363,740 - +0.6 363,773 + +0.6 363,773 + +0.6 363,774 - +0.0 363,824 + GFF2936 0.19 -0.3 363,824 + GFF2936 0.19 -0.4 363,824 + GFF2936 0.19 -2.7 363,824 + GFF2936 0.19 -0.6 363,824 + GFF2936 0.19 -2.6 363,824 + GFF2936 0.19 +0.8 363,824 + GFF2936 0.19 +1.4 363,824 + GFF2936 0.19 +1.8 363,825 - GFF2936 0.19 -0.3 363,825 - GFF2936 0.19 -1.3 363,825 - GFF2936 0.19 +0.2 363,935 + GFF2936 0.44 +0.0 363,935 + GFF2936 0.44 -1.2 363,935 + GFF2936 0.44 -0.5 363,935 + GFF2936 0.44 -0.5 363,936 - GFF2936 0.44 +0.5 364,115 + GFF2936 0.85 -1.0 364,115 + GFF2936 0.85 -1.1 364,191 + -0.6 364,191 + +1.6 364,191 + -0.4 364,191 + -0.1 364,191 + +0.8 364,191 + -0.3 364,191 + +1.1 364,191 + +0.7 364,191 + -0.9 364,191 + -0.6 364,191 + -1.4 364,191 + +1.0 364,191 + +0.7 364,191 + +0.1 364,191 + +1.1 364,191 + -0.4 364,191 + +0.0 364,191 + +0.6 364,191 + +0.9 364,192 - -0.4 364,192 - -0.7 364,192 - -0.4 364,192 - -0.1 364,192 - -2.4 364,192 - -0.2 364,192 - -0.9 364,192 - +1.9 364,192 - -0.2 364,223 + -0.2 364,223 + +1.6 364,223 + -0.7 364,224 - -0.8 364,224 - +0.1 364,271 + +1.2 364,271 + -0.7 364,271 + -0.3 364,271 + -0.1 364,271 + -1.2 364,271 + -1.9 364,271 + +0.4 364,271 + -0.3 364,271 + -0.6 364,272 - +0.8 364,272 - +1.9 364,272 - +0.6 364,272 - +0.2 364,272 - -1.6 364,272 - -0.6 364,272 - +0.1 364,272 - +0.3 364,272 - -0.7 364,272 - +0.6 364,272 - -0.5 364,272 - -0.6 364,274 + -0.7 364,274 + +0.4 364,274 + -0.7 364,274 + +1.6 364,274 + -0.6 364,274 + -0.2 364,274 + -0.2 364,274 + -0.5 364,274 + -0.4 364,274 + -1.1 364,274 + -0.5 364,275 - -0.3 364,275 - -0.6 364,275 - -0.8 364,275 - -1.9 364,275 - -0.4 364,275 - -0.3 364,275 - -0.8 364,275 - -0.5 364,275 - -0.4 364,275 - -0.2 364,280 + +0.1 364,280 + -1.3 364,280 + -0.3 364,280 + -1.1 364,280 + -1.0 364,280 + -0.2 364,280 + -1.6 364,280 + -0.6 364,280 + +0.3 364,280 + +2.9 364,280 + -1.5 364,280 + +0.1 364,280 + -0.4 364,281 - -1.9 364,281 - -0.0 364,281 - -1.6 364,281 - +0.7 364,281 - -0.9 364,281 - -1.4 364,281 - -1.1 364,346 + GFF2937 0.13 -0.7 364,346 + GFF2937 0.13 +0.1 364,346 + GFF2937 0.13 -1.5 364,346 + GFF2937 0.13 -0.1 364,346 + GFF2937 0.13 -0.1 364,346 + GFF2937 0.13 +0.4 364,346 + GFF2937 0.13 +0.2 364,346 + GFF2937 0.13 +0.2 364,346 + GFF2937 0.13 -0.3 364,346 + GFF2937 0.13 +0.0 364,346 + GFF2937 0.13 -1.0 364,347 - GFF2937 0.13 +0.8 364,347 - GFF2937 0.13 -0.1 364,347 - GFF2937 0.13 +0.4 364,347 - GFF2937 0.13 -0.2 364,347 - GFF2937 0.13 -1.5 364,347 - GFF2937 0.13 -0.0 364,347 - GFF2937 0.13 +0.2 364,347 - GFF2937 0.13 -1.9 364,347 - GFF2937 0.13 -0.2 364,347 - GFF2937 0.13 -1.2 364,347 - GFF2937 0.13 +0.1 364,347 - GFF2937 0.13 -1.2 364,370 + GFF2937 0.15 +0.7 364,370 + GFF2937 0.15 +0.2 364,370 + GFF2937 0.15 -0.9 364,370 + GFF2937 0.15 +1.3 364,370 + GFF2937 0.15 +0.7 364,370 + GFF2937 0.15 +0.4 364,370 + GFF2937 0.15 -0.2 364,370 + GFF2937 0.15 +0.5 364,371 - GFF2937 0.15 -1.1 364,371 - GFF2937 0.15 +1.3 364,371 - GFF2937 0.15 +1.6 364,371 - GFF2937 0.15 -0.2 364,371 - GFF2937 0.15 +0.9 364,661 + GFF2937 0.45 -0.5 364,661 + GFF2937 0.45 +1.0 364,662 - GFF2937 0.45 +0.2 364,662 - GFF2937 0.45 -0.7 364,662 - GFF2937 0.45 +0.3 364,685 + GFF2937 0.48 -1.2 364,685 + GFF2937 0.48 +0.2 364,685 + GFF2937 0.48 -1.1 364,686 - GFF2937 0.48 +0.6 364,686 - GFF2937 0.48 +0.1 364,686 - GFF2937 0.48 +0.4 364,686 - GFF2937 0.48 -0.7 364,686 - GFF2937 0.48 +0.7 364,748 + GFF2937 0.54 +1.3 364,748 + GFF2937 0.54 -1.2 364,748 + GFF2937 0.54 -0.5 364,748 + GFF2937 0.54 -0.4 364,748 + GFF2937 0.54 -0.0 364,748 + GFF2937 0.54 -0.0 364,748 + GFF2937 0.54 +0.4 364,748 + GFF2937 0.54 -0.6 364,748 + GFF2937 0.54 +0.1 364,748 + GFF2937 0.54 +0.3 364,748 + GFF2937 0.54 -0.2 364,748 + GFF2937 0.54 -1.6 364,748 + GFF2937 0.54 +1.6 364,748 + GFF2937 0.54 +1.6 364,748 + GFF2937 0.54 -0.3 364,748 + GFF2937 0.54 +0.4 364,748 + GFF2937 0.54 +0.4 364,748 + GFF2937 0.54 +0.8 364,748 + GFF2937 0.54 +0.6 364,748 + GFF2937 0.54 +1.3 364,748 + GFF2937 0.54 +0.0 364,748 + GFF2937 0.54 -0.2 364,748 + GFF2937 0.54 -0.7 364,749 - GFF2937 0.54 +0.8 364,749 - GFF2937 0.54 -0.7 364,749 - GFF2937 0.54 -0.4 364,749 - GFF2937 0.54 -0.4 364,749 - GFF2937 0.54 -0.5 364,749 - GFF2937 0.54 +0.8 364,749 - GFF2937 0.54 +0.2 364,749 - GFF2937 0.54 -1.3 364,749 - GFF2937 0.54 -0.6 364,749 - GFF2937 0.54 +0.6 364,749 - GFF2937 0.54 +0.1 364,749 - GFF2937 0.54 +0.6 364,749 - GFF2937 0.54 +0.3 364,749 - GFF2937 0.54 +0.2 364,749 - GFF2937 0.54 +2.1 364,749 - GFF2937 0.54 +0.6 364,749 - GFF2937 0.54 -0.1 364,749 - GFF2937 0.54 -1.3 364,749 - GFF2937 0.54 +0.2 364,749 - GFF2937 0.54 -0.3 364,749 - GFF2937 0.54 +0.2 364,749 - GFF2937 0.54 -0.3 364,749 - GFF2937 0.54 -0.1 364,749 - GFF2937 0.54 +0.4 364,749 - GFF2937 0.54 -0.2 364,750 - GFF2937 0.54 +2.3 365,260 + -1.9 365,261 - -0.4 365,261 - -1.1 365,261 - -1.0 365,261 - -0.5 365,518 + GFF2938 0.32 -0.4 365,518 + GFF2938 0.32 -0.9 365,519 - GFF2938 0.32 +2.0 365,533 + GFF2938 0.34 -1.9 365,533 + GFF2938 0.34 +0.7 365,534 - GFF2938 0.34 -0.1 366,159 + GFF2939 0.21 -0.1 366,165 + GFF2939 0.21 -2.0 366,166 - GFF2939 0.21 -1.0
Or see this region's nucleotide sequence