Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2867 and GFF2868 are separated by 24 nucleotides GFF2868 and GFF2869 are separated by 29 nucleotides
GFF2867 - DNA recombination protein RmuC, at 290,784 to 292,151
GFF2867
GFF2868 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215), at 292,176 to 293,162
GFF2868
GFF2869 - putative anion permease, at 293,192 to 294,604
GFF2869
Position (kb)
292
293
294 Strain fitness (log2 ratio)
-2
-1
0
1 at 291.217 kb on + strand, within GFF2867 at 291.217 kb on + strand, within GFF2867 at 291.217 kb on + strand, within GFF2867 at 291.217 kb on + strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.218 kb on - strand, within GFF2867 at 291.328 kb on + strand, within GFF2867 at 291.328 kb on + strand, within GFF2867 at 291.328 kb on + strand, within GFF2867 at 291.328 kb on + strand, within GFF2867 at 291.457 kb on + strand, within GFF2867 at 291.457 kb on + strand, within GFF2867 at 291.987 kb on + strand, within GFF2867 at 291.988 kb on - strand, within GFF2867 at 291.988 kb on - strand, within GFF2867 at 292.136 kb on + strand at 292.136 kb on + strand at 292.136 kb on + strand at 292.137 kb on - strand at 292.137 kb on - strand at 292.177 kb on + strand at 292.177 kb on + strand at 292.177 kb on + strand at 292.178 kb on - strand at 292.235 kb on - strand at 292.301 kb on + strand, within GFF2868 at 292.525 kb on + strand, within GFF2868 at 292.582 kb on + strand, within GFF2868 at 292.582 kb on + strand, within GFF2868 at 292.582 kb on + strand, within GFF2868 at 292.582 kb on + strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.583 kb on - strand, within GFF2868 at 292.636 kb on + strand, within GFF2868 at 292.636 kb on + strand, within GFF2868 at 292.637 kb on - strand, within GFF2868 at 292.637 kb on - strand, within GFF2868 at 292.927 kb on + strand, within GFF2868 at 292.927 kb on + strand, within GFF2868 at 292.928 kb on - strand, within GFF2868 at 292.928 kb on - strand, within GFF2868 at 292.928 kb on - strand, within GFF2868
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 291,217 + GFF2867 0.32 +1.7 291,217 + GFF2867 0.32 -0.3 291,217 + GFF2867 0.32 +0.1 291,217 + GFF2867 0.32 -0.2 291,218 - GFF2867 0.32 +0.0 291,218 - GFF2867 0.32 +0.1 291,218 - GFF2867 0.32 -0.4 291,218 - GFF2867 0.32 -0.1 291,218 - GFF2867 0.32 +0.5 291,218 - GFF2867 0.32 -0.6 291,328 + GFF2867 0.40 -0.2 291,328 + GFF2867 0.40 -0.5 291,328 + GFF2867 0.40 -0.4 291,328 + GFF2867 0.40 +0.9 291,457 + GFF2867 0.49 +0.0 291,457 + GFF2867 0.49 -0.6 291,987 + GFF2867 0.88 +0.0 291,988 - GFF2867 0.88 +0.4 291,988 - GFF2867 0.88 -0.2 292,136 + +1.0 292,136 + -2.0 292,136 + +0.5 292,137 - -0.4 292,137 - -0.2 292,177 + +0.3 292,177 + -1.5 292,177 + -1.5 292,178 - -0.4 292,235 - -0.6 292,301 + GFF2868 0.13 -0.3 292,525 + GFF2868 0.35 -0.7 292,582 + GFF2868 0.41 +0.6 292,582 + GFF2868 0.41 -2.1 292,582 + GFF2868 0.41 -0.3 292,582 + GFF2868 0.41 -0.4 292,583 - GFF2868 0.41 +0.0 292,583 - GFF2868 0.41 -1.7 292,583 - GFF2868 0.41 -0.6 292,583 - GFF2868 0.41 -0.4 292,583 - GFF2868 0.41 -1.1 292,583 - GFF2868 0.41 +0.3 292,583 - GFF2868 0.41 +0.1 292,636 + GFF2868 0.47 -0.7 292,636 + GFF2868 0.47 -0.9 292,637 - GFF2868 0.47 -2.1 292,637 - GFF2868 0.47 -0.7 292,927 + GFF2868 0.76 -0.9 292,927 + GFF2868 0.76 -0.2 292,928 - GFF2868 0.76 -0.1 292,928 - GFF2868 0.76 +0.4 292,928 - GFF2868 0.76 -0.4
Or see this region's nucleotide sequence