Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2716 and GFF2717 are separated by 19 nucleotides GFF2717 and GFF2718 overlap by 1 nucleotides GFF2718 and GFF2719 are separated by 36 nucleotides GFF2719 and GFF2720 are separated by 59 nucleotides
GFF2716 - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45), at 149,788 to 150,741
GFF2716
GFF2717 - Epimerase KguE, at 150,761 to 151,510
GFF2717
GFF2718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 151,510 to 152,265
GFF2718
GFF2719 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 152,302 to 153,099
GFF2719
GFF2720 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 153,159 to 153,812
GFF2720
Position (kb) 151
152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 150.729 kb on + strand at 150.729 kb on + strand at 150.729 kb on + strand at 150.762 kb on + strand at 150.763 kb on - strand at 150.763 kb on - strand at 150.785 kb on + strand at 150.785 kb on + strand at 150.786 kb on - strand at 150.966 kb on + strand, within GFF2717 at 150.966 kb on + strand, within GFF2717 at 150.966 kb on + strand, within GFF2717 at 151.329 kb on + strand, within GFF2717 at 151.329 kb on + strand, within GFF2717 at 151.330 kb on - strand, within GFF2717 at 151.853 kb on + strand, within GFF2718 at 151.853 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 151.892 kb on + strand, within GFF2718 at 152.219 kb on + strand at 152.219 kb on + strand at 152.220 kb on - strand at 152.292 kb on - strand at 152.303 kb on + strand at 152.304 kb on - strand at 152.471 kb on + strand, within GFF2719 at 152.711 kb on + strand, within GFF2719 at 152.711 kb on + strand, within GFF2719 at 152.910 kb on - strand, within GFF2719 at 152.945 kb on + strand, within GFF2719 at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.104 kb on + strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.105 kb on - strand at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.235 kb on + strand, within GFF2720 at 153.236 kb on - strand, within GFF2720
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 150,729 + +0.8 150,729 + -0.6 150,729 + +1.8 150,762 + +0.5 150,763 - +0.9 150,763 - +1.0 150,785 + -0.2 150,785 + +0.6 150,786 - -0.6 150,966 + GFF2717 0.27 +0.1 150,966 + GFF2717 0.27 -0.2 150,966 + GFF2717 0.27 +0.3 151,329 + GFF2717 0.76 -0.2 151,329 + GFF2717 0.76 -0.1 151,330 - GFF2717 0.76 -0.0 151,853 + GFF2718 0.45 -0.5 151,853 + GFF2718 0.45 -0.6 151,892 + GFF2718 0.51 -0.9 151,892 + GFF2718 0.51 -0.1 151,892 + GFF2718 0.51 -0.7 152,219 + -1.0 152,219 + +1.2 152,220 - +1.2 152,292 - +2.2 152,303 + -0.7 152,304 - -0.2 152,471 + GFF2719 0.21 -1.9 152,711 + GFF2719 0.51 -0.5 152,711 + GFF2719 0.51 +0.2 152,910 - GFF2719 0.76 -0.5 152,945 + GFF2719 0.81 -2.1 153,104 + +0.2 153,104 + +0.3 153,104 + -0.4 153,104 + -2.8 153,105 - -1.1 153,105 - +0.2 153,105 - -0.4 153,105 - -0.4 153,105 - +0.7 153,105 - +0.2 153,235 + GFF2720 0.12 -0.7 153,235 + GFF2720 0.12 -1.3 153,235 + GFF2720 0.12 +0.6 153,236 - GFF2720 0.12 -1.2
Or see this region's nucleotide sequence