Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2422 and GFF2423 are separated by 36 nucleotides GFF2423 and GFF2424 overlap by 4 nucleotides GFF2424 and GFF2425 overlap by 4 nucleotides
GFF2422 - Polysaccharide deacetylase, at 473,394 to 474,218
GFF2422
GFF2423 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 474,255 to 475,046
GFF2423
GFF2424 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 475,043 to 475,894
GFF2424
GFF2425 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 475,891 to 476,982
GFF2425
Position (kb) 475
476 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.603 kb on + strand, within GFF2423 at 474.604 kb on - strand, within GFF2423 at 474.607 kb on + strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.608 kb on - strand, within GFF2423 at 474.676 kb on + strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.680 kb on - strand, within GFF2423 at 474.784 kb on + strand, within GFF2423 at 474.785 kb on - strand, within GFF2423 at 474.931 kb on + strand, within GFF2423 at 474.932 kb on - strand, within GFF2423 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.170 kb on + strand, within GFF2424 at 475.194 kb on + strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.195 kb on - strand, within GFF2424 at 475.339 kb on - strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.626 kb on + strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.627 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.630 kb on - strand, within GFF2424 at 475.710 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.713 kb on + strand, within GFF2424 at 475.714 kb on - strand, within GFF2424 at 475.737 kb on + strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.738 kb on - strand, within GFF2424 at 475.783 kb on - strand, within GFF2424 at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.928 kb on + strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 475.929 kb on - strand at 476.042 kb on + strand, within GFF2425 at 476.042 kb on + strand, within GFF2425 at 476.043 kb on - strand, within GFF2425 at 476.318 kb on + strand, within GFF2425 at 476.319 kb on - strand, within GFF2425 at 476.319 kb on - strand, within GFF2425
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 474,603 + GFF2423 0.44 +0.6 474,603 + GFF2423 0.44 -0.7 474,603 + GFF2423 0.44 +0.1 474,603 + GFF2423 0.44 +1.3 474,603 + GFF2423 0.44 +0.8 474,604 - GFF2423 0.44 +0.4 474,607 + GFF2423 0.44 -0.3 474,608 - GFF2423 0.45 +1.7 474,608 - GFF2423 0.45 -0.8 474,676 + GFF2423 0.53 +0.4 474,680 - GFF2423 0.54 -0.9 474,680 - GFF2423 0.54 +1.7 474,680 - GFF2423 0.54 +0.9 474,784 + GFF2423 0.67 +0.3 474,785 - GFF2423 0.67 -0.3 474,931 + GFF2423 0.85 +0.1 474,932 - GFF2423 0.85 +0.7 475,170 + GFF2424 0.15 -1.3 475,170 + GFF2424 0.15 +0.3 475,170 + GFF2424 0.15 +0.7 475,194 + GFF2424 0.18 -0.2 475,195 - GFF2424 0.18 -1.1 475,195 - GFF2424 0.18 -0.0 475,339 - GFF2424 0.35 -0.0 475,626 + GFF2424 0.68 +0.3 475,626 + GFF2424 0.68 -0.6 475,626 + GFF2424 0.68 -0.3 475,626 + GFF2424 0.68 -0.2 475,627 - GFF2424 0.69 +1.1 475,627 - GFF2424 0.69 +0.1 475,627 - GFF2424 0.69 -2.6 475,627 - GFF2424 0.69 +0.2 475,627 - GFF2424 0.69 -0.9 475,630 - GFF2424 0.69 +0.9 475,630 - GFF2424 0.69 -0.1 475,710 + GFF2424 0.78 -0.6 475,713 + GFF2424 0.79 -0.6 475,713 + GFF2424 0.79 +0.3 475,713 + GFF2424 0.79 -0.0 475,714 - GFF2424 0.79 -0.9 475,737 + GFF2424 0.81 -0.6 475,738 - GFF2424 0.82 +2.0 475,738 - GFF2424 0.82 -0.1 475,738 - GFF2424 0.82 +0.6 475,783 - GFF2424 0.87 -3.0 475,928 + +0.1 475,928 + -0.6 475,928 + -0.4 475,928 + -0.7 475,929 - +0.1 475,929 - -0.8 475,929 - -0.5 475,929 - +1.0 475,929 - +0.2 475,929 - -0.7 475,929 - -0.1 476,042 + GFF2425 0.14 +0.3 476,042 + GFF2425 0.14 -1.2 476,043 - GFF2425 0.14 -0.1 476,318 + GFF2425 0.39 -0.6 476,319 - GFF2425 0.39 +0.4 476,319 - GFF2425 0.39 +0.6
Or see this region's nucleotide sequence