Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2379 and GFF2380 are separated by 7 nucleotides GFF2380 and GFF2381 are separated by 43 nucleotides GFF2381 and GFF2382 are separated by 59 nucleotides GFF2382 and GFF2383 overlap by 1 nucleotides
GFF2379 - Serine/threonine protein kinase, at 433,542 to 434,549
GFF2379
GFF2380 - Protein phosphatase 2C-like, at 434,557 to 435,465
GFF2380
GFF2381 - Ribonuclease PH (EC 2.7.7.56), at 435,509 to 436,243
GFF2381
GFF2382 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.66), at 436,303 to 436,908
GFF2382
GFF2383 - Oxygen-independent coproporphyrinogen-III oxidase-like protein YggW, at 436,908 to 438,170
GFF2383
Position (kb) 435
436
437 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 434.623 kb on + strand at 434.623 kb on + strand at 434.624 kb on - strand at 434.624 kb on - strand at 434.624 kb on - strand at 434.629 kb on + strand at 434.629 kb on + strand at 434.629 kb on + strand at 434.629 kb on + strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.630 kb on - strand at 434.732 kb on - strand, within GFF2380 at 434.818 kb on + strand, within GFF2380 at 434.923 kb on + strand, within GFF2380 at 434.923 kb on + strand, within GFF2380 at 434.923 kb on + strand, within GFF2380 at 434.923 kb on + strand, within GFF2380 at 434.923 kb on + strand, within GFF2380 at 434.924 kb on - strand, within GFF2380 at 434.924 kb on - strand, within GFF2380 at 434.924 kb on - strand, within GFF2380 at 434.924 kb on - strand, within GFF2380 at 435.707 kb on + strand, within GFF2381 at 436.049 kb on + strand, within GFF2381 at 436.050 kb on - strand, within GFF2381 at 436.050 kb on - strand, within GFF2381 at 436.254 kb on + strand at 436.254 kb on + strand at 436.255 kb on - strand at 436.255 kb on - strand at 436.255 kb on - strand at 436.255 kb on - strand at 436.255 kb on - strand at 436.255 kb on - strand at 436.302 kb on + strand at 436.303 kb on - strand at 436.303 kb on - strand at 436.455 kb on + strand, within GFF2382 at 436.558 kb on + strand, within GFF2382 at 436.559 kb on - strand, within GFF2382 at 436.562 kb on - strand, within GFF2382 at 436.562 kb on - strand, within GFF2382 at 436.562 kb on - strand, within GFF2382 at 436.562 kb on - strand, within GFF2382 at 436.562 kb on - strand, within GFF2382 at 436.783 kb on + strand, within GFF2382 at 436.783 kb on + strand, within GFF2382 at 436.783 kb on + strand, within GFF2382 at 436.783 kb on + strand, within GFF2382 at 436.783 kb on + strand, within GFF2382 at 436.784 kb on - strand, within GFF2382 at 437.028 kb on + strand at 437.028 kb on + strand at 437.029 kb on - strand at 437.029 kb on - strand at 437.029 kb on - strand at 437.029 kb on - strand at 437.029 kb on - strand at 437.029 kb on - strand at 437.065 kb on - strand, within GFF2383 at 437.136 kb on + strand, within GFF2383 at 437.136 kb on + strand, within GFF2383 at 437.136 kb on + strand, within GFF2383 at 437.136 kb on + strand, within GFF2383 at 437.137 kb on - strand, within GFF2383 at 437.137 kb on - strand, within GFF2383 at 437.137 kb on - strand, within GFF2383 at 437.192 kb on + strand, within GFF2383
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 434,623 + -1.7 434,623 + -0.5 434,624 - +0.4 434,624 - -1.1 434,624 - -0.3 434,629 + -0.3 434,629 + +0.0 434,629 + -0.2 434,629 + +0.7 434,630 - -0.1 434,630 - -1.0 434,630 - -0.2 434,630 - +0.7 434,630 - +0.7 434,630 - -1.1 434,630 - +0.2 434,630 - -1.0 434,732 - GFF2380 0.19 +0.0 434,818 + GFF2380 0.29 -0.1 434,923 + GFF2380 0.40 -0.6 434,923 + GFF2380 0.40 -0.3 434,923 + GFF2380 0.40 -0.6 434,923 + GFF2380 0.40 +0.7 434,923 + GFF2380 0.40 -0.1 434,924 - GFF2380 0.40 -1.4 434,924 - GFF2380 0.40 -0.6 434,924 - GFF2380 0.40 -1.0 434,924 - GFF2380 0.40 -0.8 435,707 + GFF2381 0.27 +0.3 436,049 + GFF2381 0.73 -1.8 436,050 - GFF2381 0.74 -1.3 436,050 - GFF2381 0.74 -2.8 436,254 + -0.3 436,254 + -0.6 436,255 - -1.6 436,255 - -0.9 436,255 - -1.0 436,255 - -0.4 436,255 - +0.2 436,255 - -0.3 436,302 + -0.5 436,303 - -0.0 436,303 - -1.0 436,455 + GFF2382 0.25 +0.2 436,558 + GFF2382 0.42 +0.4 436,559 - GFF2382 0.42 -1.3 436,562 - GFF2382 0.43 -0.4 436,562 - GFF2382 0.43 -0.8 436,562 - GFF2382 0.43 -0.6 436,562 - GFF2382 0.43 -0.6 436,562 - GFF2382 0.43 -1.6 436,783 + GFF2382 0.79 -0.6 436,783 + GFF2382 0.79 -2.2 436,783 + GFF2382 0.79 -1.5 436,783 + GFF2382 0.79 -0.3 436,783 + GFF2382 0.79 +2.0 436,784 - GFF2382 0.79 -1.7 437,028 + -0.3 437,028 + -0.3 437,029 - -0.2 437,029 - +0.2 437,029 - -0.4 437,029 - +0.3 437,029 - +0.5 437,029 - -0.7 437,065 - GFF2383 0.12 -1.7 437,136 + GFF2383 0.18 -0.2 437,136 + GFF2383 0.18 +0.4 437,136 + GFF2383 0.18 +0.3 437,136 + GFF2383 0.18 +0.1 437,137 - GFF2383 0.18 -1.0 437,137 - GFF2383 0.18 -1.1 437,137 - GFF2383 0.18 -0.1 437,192 + GFF2383 0.22 -0.1
Or see this region's nucleotide sequence