Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF226 and GFF227 overlap by 1 nucleotides GFF227 and GFF228 are separated by 147 nucleotides
GFF226 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 252,852 to 254,492
GFF226
GFF227 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 254,492 to 255,532
GFF227
GFF228 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 255,680 to 256,720
GFF228
Position (kb)
254
255
256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.612 kb on + strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.613 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.718 kb on - strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.855 kb on + strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 253.856 kb on - strand, within GFF226 at 254.146 kb on + strand, within GFF226 at 254.146 kb on + strand, within GFF226 at 254.147 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.273 kb on - strand, within GFF226 at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.474 kb on + strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.475 kb on - strand at 254.611 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.820 kb on + strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.821 kb on - strand, within GFF227 at 254.859 kb on + strand, within GFF227 at 254.859 kb on + strand, within GFF227 at 254.860 kb on - strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.952 kb on + strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.953 kb on - strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.975 kb on + strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 254.976 kb on - strand, within GFF227 at 255.021 kb on + strand, within GFF227 at 255.021 kb on + strand, within GFF227 at 255.022 kb on - strand, within GFF227 at 255.108 kb on + strand, within GFF227 at 255.108 kb on + strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.109 kb on - strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.201 kb on + strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.202 kb on - strand, within GFF227 at 255.223 kb on - strand, within GFF227 at 255.237 kb on + strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.238 kb on - strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.279 kb on + strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.280 kb on - strand, within GFF227 at 255.291 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.336 kb on + strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.337 kb on - strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.357 kb on + strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.358 kb on - strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.361 kb on + strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.362 kb on - strand, within GFF227 at 255.372 kb on + strand, within GFF227 at 255.373 kb on - strand, within GFF227 at 255.373 kb on - strand, within GFF227 at 255.511 kb on + strand at 255.511 kb on + strand at 255.511 kb on + strand at 255.511 kb on + strand at 255.511 kb on + strand at 255.511 kb on + strand at 255.511 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.710 kb on + strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.711 kb on - strand at 255.714 kb on + strand at 255.714 kb on + strand at 255.714 kb on + strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.765 kb on + strand at 255.765 kb on + strand at 255.765 kb on + strand at 255.765 kb on + strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.790 kb on - strand, within GFF228 at 255.814 kb on - strand, within GFF228 at 255.857 kb on - strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 256.098 kb on + strand, within GFF228 at 256.099 kb on - strand, within GFF228 at 256.099 kb on - strand, within GFF228 at 256.123 kb on + strand, within GFF228 at 256.230 kb on + strand, within GFF228 at 256.231 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.333 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 253,612 + GFF226 0.46 -0.6 253,612 + GFF226 0.46 -1.0 253,612 + GFF226 0.46 -0.2 253,612 + GFF226 0.46 -0.9 253,612 + GFF226 0.46 +0.7 253,612 + GFF226 0.46 -0.3 253,613 - GFF226 0.46 -0.1 253,613 - GFF226 0.46 -1.3 253,718 - GFF226 0.53 -1.9 253,718 - GFF226 0.53 -0.9 253,718 - GFF226 0.53 -0.7 253,855 + GFF226 0.61 -2.6 253,855 + GFF226 0.61 -0.3 253,855 + GFF226 0.61 -2.0 253,855 + GFF226 0.61 -0.6 253,856 - GFF226 0.61 -0.5 253,856 - GFF226 0.61 -2.6 253,856 - GFF226 0.61 +0.7 253,856 - GFF226 0.61 -1.3 253,856 - GFF226 0.61 +1.3 253,856 - GFF226 0.61 -0.8 253,856 - GFF226 0.61 -0.7 253,856 - GFF226 0.61 -1.0 254,146 + GFF226 0.79 -0.7 254,146 + GFF226 0.79 -0.1 254,147 - GFF226 0.79 -0.2 254,273 - GFF226 0.87 +1.0 254,273 - GFF226 0.87 -1.3 254,273 - GFF226 0.87 -0.2 254,474 + +0.7 254,474 + -1.4 254,474 + +0.3 254,474 + -0.5 254,474 + -0.3 254,474 + +0.9 254,474 + -0.5 254,474 + +2.0 254,475 - -1.8 254,475 - -0.8 254,475 - +2.5 254,475 - +0.7 254,475 - +0.1 254,475 - +0.6 254,475 - -1.0 254,611 + GFF227 0.11 -0.1 254,820 + GFF227 0.32 +0.2 254,820 + GFF227 0.32 -1.1 254,820 + GFF227 0.32 -0.1 254,820 + GFF227 0.32 -0.4 254,820 + GFF227 0.32 -0.1 254,820 + GFF227 0.32 -1.2 254,820 + GFF227 0.32 -1.2 254,820 + GFF227 0.32 +0.3 254,820 + GFF227 0.32 +1.3 254,821 - GFF227 0.32 -0.9 254,821 - GFF227 0.32 -1.1 254,821 - GFF227 0.32 -1.0 254,821 - GFF227 0.32 -0.6 254,821 - GFF227 0.32 +0.2 254,821 - GFF227 0.32 +0.1 254,859 + GFF227 0.35 -1.3 254,859 + GFF227 0.35 -1.2 254,860 - GFF227 0.35 -0.5 254,952 + GFF227 0.44 -1.0 254,952 + GFF227 0.44 -3.2 254,952 + GFF227 0.44 -0.7 254,952 + GFF227 0.44 -1.0 254,952 + GFF227 0.44 -0.7 254,952 + GFF227 0.44 +0.8 254,953 - GFF227 0.44 -0.4 254,953 - GFF227 0.44 -0.7 254,953 - GFF227 0.44 -1.3 254,953 - GFF227 0.44 -0.5 254,953 - GFF227 0.44 -1.6 254,953 - GFF227 0.44 -0.6 254,953 - GFF227 0.44 +0.2 254,975 + GFF227 0.46 -0.5 254,975 + GFF227 0.46 -1.2 254,975 + GFF227 0.46 -1.8 254,975 + GFF227 0.46 -1.2 254,975 + GFF227 0.46 -0.9 254,975 + GFF227 0.46 +1.7 254,975 + GFF227 0.46 +1.1 254,975 + GFF227 0.46 -0.7 254,976 - GFF227 0.46 -2.3 254,976 - GFF227 0.46 +0.4 254,976 - GFF227 0.46 -1.5 254,976 - GFF227 0.46 -1.2 255,021 + GFF227 0.51 -1.0 255,021 + GFF227 0.51 -1.4 255,022 - GFF227 0.51 -0.5 255,108 + GFF227 0.59 -0.3 255,108 + GFF227 0.59 -1.5 255,109 - GFF227 0.59 +1.6 255,109 - GFF227 0.59 -0.7 255,109 - GFF227 0.59 -0.3 255,201 + GFF227 0.68 -0.1 255,201 + GFF227 0.68 -1.3 255,201 + GFF227 0.68 -1.0 255,201 + GFF227 0.68 +0.6 255,201 + GFF227 0.68 -0.5 255,201 + GFF227 0.68 -1.2 255,201 + GFF227 0.68 -0.1 255,201 + GFF227 0.68 +0.6 255,202 - GFF227 0.68 -1.6 255,202 - GFF227 0.68 -0.7 255,202 - GFF227 0.68 -0.3 255,202 - GFF227 0.68 +0.5 255,223 - GFF227 0.70 -2.1 255,237 + GFF227 0.72 -0.9 255,238 - GFF227 0.72 -1.5 255,238 - GFF227 0.72 +1.7 255,238 - GFF227 0.72 -0.6 255,279 + GFF227 0.76 -0.2 255,279 + GFF227 0.76 +2.6 255,279 + GFF227 0.76 -0.5 255,279 + GFF227 0.76 -0.9 255,280 - GFF227 0.76 -0.7 255,280 - GFF227 0.76 +0.2 255,280 - GFF227 0.76 -0.6 255,291 + GFF227 0.77 -0.3 255,336 + GFF227 0.81 -0.9 255,336 + GFF227 0.81 -0.8 255,336 + GFF227 0.81 -0.6 255,337 - GFF227 0.81 -0.8 255,337 - GFF227 0.81 -0.0 255,337 - GFF227 0.81 +1.1 255,337 - GFF227 0.81 +0.1 255,357 + GFF227 0.83 -0.1 255,357 + GFF227 0.83 -2.5 255,357 + GFF227 0.83 +0.2 255,357 + GFF227 0.83 +1.6 255,357 + GFF227 0.83 -1.8 255,358 - GFF227 0.83 -1.6 255,358 - GFF227 0.83 -1.0 255,358 - GFF227 0.83 -1.2 255,358 - GFF227 0.83 +0.1 255,358 - GFF227 0.83 -1.3 255,358 - GFF227 0.83 -0.9 255,358 - GFF227 0.83 +0.5 255,358 - GFF227 0.83 -1.5 255,361 + GFF227 0.83 -0.2 255,361 + GFF227 0.83 -1.5 255,361 + GFF227 0.83 -0.2 255,361 + GFF227 0.83 -0.6 255,361 + GFF227 0.83 -1.0 255,362 - GFF227 0.84 -2.3 255,362 - GFF227 0.84 -0.6 255,362 - GFF227 0.84 -0.3 255,372 + GFF227 0.85 -1.2 255,373 - GFF227 0.85 -0.9 255,373 - GFF227 0.85 -0.8 255,511 + -2.0 255,511 + +0.4 255,511 + -0.2 255,511 + -0.7 255,511 + -0.3 255,511 + +0.3 255,511 + -0.9 255,710 + -0.2 255,710 + -1.3 255,710 + -0.3 255,710 + -2.7 255,710 + -0.5 255,710 + -0.7 255,710 + -1.3 255,710 + -1.0 255,710 + -0.7 255,710 + -0.5 255,710 + +1.3 255,711 - -0.7 255,711 - -0.7 255,711 - -1.0 255,711 - -1.9 255,711 - -0.9 255,711 - -0.0 255,711 - +0.3 255,711 - -0.6 255,711 - -0.6 255,711 - +0.5 255,711 - -0.6 255,711 - -1.5 255,714 + -0.7 255,714 + -1.4 255,714 + -1.1 255,715 - +0.4 255,715 - -0.5 255,715 - -0.1 255,715 - -0.3 255,715 - -0.3 255,715 - +0.6 255,765 + -2.7 255,765 + -1.3 255,765 + -0.6 255,765 + -0.7 255,766 - -1.2 255,766 - -0.0 255,766 - +0.5 255,766 - -2.5 255,766 - -0.5 255,790 - GFF228 0.11 -1.3 255,814 - GFF228 0.13 +0.7 255,857 - GFF228 0.17 -1.0 255,993 + GFF228 0.30 -0.9 255,993 + GFF228 0.30 -2.3 255,993 + GFF228 0.30 +0.6 255,993 + GFF228 0.30 -0.7 255,993 + GFF228 0.30 -2.4 255,993 + GFF228 0.30 -0.7 255,994 - GFF228 0.30 -0.3 255,994 - GFF228 0.30 +0.9 255,994 - GFF228 0.30 -0.5 255,994 - GFF228 0.30 -0.3 255,994 - GFF228 0.30 -1.4 255,994 - GFF228 0.30 -3.6 255,994 - GFF228 0.30 -1.0 256,098 + GFF228 0.40 +1.3 256,099 - GFF228 0.40 -0.3 256,099 - GFF228 0.40 -0.4 256,123 + GFF228 0.43 -0.5 256,230 + GFF228 0.53 -1.3 256,231 - GFF228 0.53 +0.1 256,321 - GFF228 0.62 +0.3 256,321 - GFF228 0.62 -0.1 256,321 - GFF228 0.62 -0.8 256,321 - GFF228 0.62 -3.2 256,333 - GFF228 0.63 -1.5 256,486 - GFF228 0.77 -0.7 256,486 - GFF228 0.77 -1.0 256,486 - GFF228 0.77 -2.5
Or see this region's nucleotide sequence